Assays

255 Assays visible to you, out of a total of 466

Temperature degradation of BPG, GAP and DHAP

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

test

Contributor: Holger Janssen

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

No description specified

Contributor: John Raedts

Assay type: Metabolomics

Technology type: Technology Type

Snapshots: No snapshots

We developed a new metabolomics protocol, which involved a comparison of different harvesting techniques, quenching solutions and extraction methods. Cell leakage and metabolite recovery was determined by ATP measurements

Contributor: John Raedts

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Using TFinfer2 to analyse data from "Characterization of MG1655 and mutant strains under conditions of glucose excess and limitation"

Contributor: Botond Cseke

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

No description specified

B. subtilis was grown in SMM media with glucose as carbon source and the samples for RNA were harvested OD578nm- 1.0). The stress conditions that were applied over here are growthat 57°C, 16°C, 1.2M Nacl and 37°C(control).
All the samples were analysed for transcriptome as biological triplicates.

B. subtilis was grown in M9 media with glucose as carbon source and the samples were harvested during exponential phase (OD600nm- 0.4), early stationary phase(OD600nm- 1.3), late stationary phase(OD600nm- 1.0).
All the samples were analysed for transcriptome as biological triplicates.

No description specified
No description specified

Contributor: Falko Krause

Assay type: Transcriptomics

Technology type: Microarray

Snapshots: No snapshots

Time courses of the internal pH changes under the conditions of Navarrete et al. (2010) will be obtained. The usage of different mutants (transport systems and regulation factors) will reveal the influence and key systems of pH regulation.

To estimate the changes of internal pH, the pH-dependent variant of GFP pHluorin is expressed in cells from a multicopy plasmid, and the changes in cell fluorescence are monitored during 5 hours of incubation in YNB-F growth media without added potassium.

Contributor: Falko Krause

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Time-dependent simulations of the dynamic switch between acidogenesis and solventogenesis based on the metabolic network and pH-dependent regulation of the enzymes.

Contributor: Sara Jabbari

Biological problem addressed: Metabolic Network

Snapshots: No snapshots

Kinetic characterisation en mathematical modelling of TPI.

Contributor: Dawie Van Niekerk

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Mathematical model for TPI kinetics, saturation with GAP and DHAP, and inhibition by 3PG and PEP

Contributor: Jacky Snoep

Biological problem addressed: Enzymology

Snapshots: No snapshots

No description specified

This assay involved the determination of transcriptional profiles at 0, 2, 5, 10, 15 and 20 minutes through aerobic to anaerobic gas transitions and anaerobic to aerobic gas transitions. In each case an aerobic or anaerobic steady state was created, RNA sampled (0 min) and then the gas supply changed. RNA samples were then taken from the time at which the gas supply was changed.

For anaerobic conditions 5% CO2, 95% N2 was used.

The full transcriptional dataset is available from ArrayExpress
...

The transcriptional profiles of steady state E. coli cultures at a range of aerobiosis levels were determined. Two biological replicates and two technical replicates were carried out. Microarrays were carried out in a reference style (i.e. RNA vs a gDNA reference).

Transcriptome analysis for the samples harvested from Chemostat cultivated samples.

No description specified

Contributor: Sebastian Curth

Assay type: Transcriptional Profiling

Technology type: Microarray

Snapshots: No snapshots

No description specified

Contributor: Jay Moore

Assay type: Transcriptomics

Technology type: Custom Array

Snapshots: No snapshots

Assay for the isolation of intact Plasmodium falciparum trophozoites from infected red blood cells and the preparation of a cell free extract that can be used for kinetic analyses.

Contributor: Dawie Van Niekerk

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

This assay is designed to obtain the in vitro kinetic data of T. brucei recombinant trypanothione synthetase. The enzyme catalyzes the ATP-dependent ligation of spermidine (Spd) and GSH to generate glutathionylspermidine (Gsp) and also of Gsp and GSH to finally produce trypanothione (T(SH)2). The data was obtained in an spectrophotometric assay that links ADP production with NADH consumption through the piruvte kinase and lactate dehydrogenase.

TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of glutathione, and spermidine.

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