Assays

255 Assays visible to you, out of a total of 466

Using Taverna for mining and MATLAB for conversion into specific formats (cytoscape, SBTOOLBOX2)

In this experiment we glucose-pulsed an E. faecalis cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catqabolism of E. faecalis

Contributor: Martijn Bekker

Assay type: Metabolomics

Technology type: Progressive Curve Experiment

Snapshots: No snapshots

In this experiment we glucose-pulsed an L. lactiss cultures re-suspended in 100 mM MES buffer at pH 6.5. Samples were taken in time to study intra- and extracellular metabolites. These data are used to construct a kinetic model of the catabolism of E. L. lactis

Contributor: Martijn Bekker

Assay type: Metabolomics

Technology type: Enzymatic Activity Measurements

Snapshots: No snapshots

No description specified

Contributor: Dawie Van Niekerk

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

No description specified
No description specified

Contributor: Sebastian Henkel

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Measurements on Km, Vmax and allosteric activation or inhibition of the main L-lactate dehydrogenase

Contributor: Martijn Bekker

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Measurements on Km, Vmax and allosteric activation or inhibition of the heterologously expressed (E. coli) and purifiied main L-lactate dehydrogenase

Artificial transcription elongation compexes are assembled in vitro using synthetic deoxy-oligonucleotides (representing template and non template DNA strands), radiolabelled RNA (representing nascent transcript) and purified RNA polymerase. After high salt wash the incorrect NTP is added and reaction is allowed to proceed for the various amounts of time. Reaction is stopped by addition of formamide-containing loading solution and the products are resolved on high-percentage denaturing polyacryamide
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Contributor: Nikolay Zenkin

Assay type: Reactomics

Technology type: Enzymatic Activity Measurements

Snapshots: No snapshots

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