Transcriptional profiling of E. coli during anaerobic to aerobic and aerobic to anaerobic transitions

This assay involved the determination of transcriptional profiles at 0, 2, 5, 10, 15 and 20 minutes through aerobic to anaerobic gas transitions and anaerobic to aerobic gas transitions. In each case an aerobic or anaerobic steady state was created, RNA sampled (0 min) and then the gas supply changed. RNA samples were then taken from the time at which the gas supply was changed.

For anaerobic conditions 5% CO2, 95% N2 was used.

The full transcriptional dataset is available from ArrayExpress (accession numbers E-MTAB-996 and E-MTAB-285)

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Created: 30th Apr 2010 at 15:07

Last updated: 8th Nov 2017 at 14:21

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