Traget verification/ Specificity of IAA in glycolysis
SEEK ID: https://fairdomhub.org/assays/2141
Experimental assay
Projects: Glucose metabolism in cancer cell lines
Investigation: Metabolic control analysis of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and hexokinase (HK) in breast cancer cell lines
Study: Iodoacetic acid (IAA) titrations in cancer cell lines
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Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Created: 13th Jul 2023 at 13:08
Last updated: 13th Jul 2023 at 13:55
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Projects: SulfoSys, SulfoSys - Biotec, Glucose metabolism in cancer cell lines
Institutions: University Duisburg-Essen, Stellenbosch University
The Snoep Lab’s core research efforts are in Computational Systems Biology; a combined experimental, modeling and theoretical approach to quantitatively understand the functional behavior of Biological Systems resulting from the characteristics of their components. Our main focus is on metabolism, of human pathogens such as Plasmodium falciparum, Mycobacterium tuberculosis, but also of breast cancer cell lines, and on modelling disease states such as glucose homeostatis in type 2 diabetes, and ...
Projects: Whole body modelling of glucose metabolism in malaria patients, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications, Glucose metabolism in cancer cell lines
Web page: http://www.sun.ac.za/english/faculty/science/biochemistry/research/snoep-group
Up-regulation of glycolytic flux (glucose to lactate conversion), also known as the “Warburg effect”, has been associated with 90% of cancers. We implemented a systems biology approach in order to improve the overall understanding of the “Warburg effect” as well as identifying drug targets within the glycolytic pathway.
Programme: SARCHI: Mechanistic modelling of health and epidemiology
Public web page: Not specified
Submitter: Theresa Kouril
Studies: 3-Bromopyruvate (3BrP) titrations, Data analysis and calculation of control coefficients, Iodoacetic acid (IAA) titrations in cancer cell lines
Assays: GAPDH and flux inhibition in MCF7 and MDA231cells, HK, GAPDH and flux inhibition in MDA231 cells, Incubation time for inhibitor treatment, Target verification/ Specificity of 3BrP in glycolysis, Traget verification/ Specificity of IAA in glycolysis
