Assays

255 Assays visible to you, out of a total of 466

BPG degradation at 70C

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

Collection for experimental SOPs

Genomics data, for L.ferriphilum
Sequencing of the genome and functional annotation

Contributor: Malte Herold

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Measurements of external metabolites based on growth curve data.
Flux estimates for uptake of external metabolites such as glucose and production rates for external metabolites lactate and acetate

Contributor: Niels Zondervan

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically

No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

RNAseq data for L.ferriphilum samples

No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Enzymatic Activity Measurements

Snapshots: No snapshots

No description specified
No description specified

Contributor: Dawie Van Niekerk

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Mutants with a linear respiratory chain consisting of NADH Dehydrogenase II and one of the terminal oxidases cytochrom bo, cytochrome bd I or cytochrome bd II were growth in chemostats with defined oxygen supply. The amounts of biomass formed and of acetate and formate produced were determined.

Mutants with linear respiratory chains were grown under SUMO chemostat conditions at different defined aerobiosis levels. The ArcA phoshorylation state as determined.

Contributor: Katja Bettenbrock

Assay type: Experimental Assay Type

Technology type: Gel Electrophoresis

Snapshots: No snapshots

Experimental data for the conversion of 3PG to F6P and the gluconeogenic pathway intermediates

Kinetic characterisation of fructose 1,6-bisphosphate aldolase phosphatase

Mutant strains with linear electron transport chain were grown in chemostat cultures at different defined aerobiosis levels. Expression of selected genes was determined by Real Time RT-PCR

No description specified

Genes are transcribed in polysictronic messages (pre-mRNA) that are destined for either maturation into mRNAs, or degradation. Since transcription regulation is non-existent with few exceptions, the rate of pre-mRNA processing, together with mRNA decay and translation rates, are believed to control gene expression. In this assay, 2T1 blood form trypanosomes are subject to treatment by ActinomycinD for 5 minutes, inhibiting transcription. The cells are harvested, depleted for ribosomal RNA, and
...

Contributor: Abeer Fadda

Assay type: Transcriptomics

Technology type: Rna-seq

Snapshots: No snapshots

No description specified
No description specified

Contributor: Dawie Van Niekerk

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Temperature degradation of BPG, GAP and DHAP

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

Assay for the isolation of intact Plasmodium falciparum trophozoites from infected red blood cells and the preparation of a cell free extract that can be used for kinetic analyses.

Contributor: Dawie Van Niekerk

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of glutathione, and spermidine.

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