SOPs

149 SOPs visible to you, out of a total of 243

Metabolic networks with gene expression are researched under very different banners with different techniques. For example, there are the dynamic enzyme-cost Flux Balance Analysis (deFBA) [1], conditional Flux Balance Analysis [2], Metabolism and Expression models (ME models) [3], Resource Balance Analysis [4], etc. At their core, these methods can all understood as Resource Allocation Models (RAM) and while investigating their potential and their results, we encountered the problem of sharing
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Creators: Henning Lindhorst, Alexandra-M. Reimers

Contributor: Henning Lindhorst

Extraction and quantitative analysis of proteins from FFPE tissue

Creators: Olga Krebs, Ruben Van Heck

Contributor: Olga Krebs

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

Method for transformation of plasmids into Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

Method for synthesis of LAB-medium sued for the SYSMO-LAB project

Creator: Martijn Bekker

Contributor: JERM

Method for analysis of various organic acids in the medium

Creator: Martijn Bekker

Contributor: JERM

Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis

Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham

Contributor: Theresa Kouril

No description specified

Creators: Theresa Kouril, Andreas Albersmeier, CeBiTech, University Bielefeld, Germany; Jörn Kalinowski, CeBiTech, University Bielefeld;

Contributor: Theresa Kouril

A Standard Operating Procedure (SOP) is a document consisting of step-by-step information on how to execute a task. An existing SOP may need to just be modified and updated.

Creator: Olga Krebs

Contributor: JERM

No description specified

Creator: Tomas Fiedler

Contributor: Tomas Fiedler

No description specified

Creator: Tomas Fiedler

Contributor: Tomas Fiedler

Protocol for applying a glucose perturbation in Streptococcus pyogenes.

Creator: Martijn Bekker

Contributor: Martijn Bekker

Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.

Creator: Martijn Bekker

Contributor: Martijn Bekker

No description specified

Creator: Martijn Bekker

Contributor: JERM

This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.

Creator: Martijn Bekker

Contributor: Martijn Bekker

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

Creator: Martijn Bekker

Contributor: Martijn Bekker

A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.

Creator: Martijn Bekker

Contributor: Martijn Bekker

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for OptKnock.

Creator: Natalie Stanford

Contributor: Natalie Stanford

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236).

Creator: Natalie Stanford

Contributor: Natalie Stanford

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for RobustKnock.

Creator: Natalie Stanford

Contributor: Natalie Stanford

SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of G3PDH activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of ALD activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of glucose transport activity in intact trophozoites.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

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