P.ENG-230912-V1_In-Vivo-Comet-Assay-Protocol.docx Version 1
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In Vivo Comet Assay Protocol.docx
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Created: 13th Sep 2023 at 17:26
Last updated: 3rd Nov 2023 at 14:00
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Version 1 (earliest) Created 13th Sep 2023 at 17:26 by Charles Demurjian
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Projects: Hi-IMPAcTB, MIT SRP, MetNet, Endometriosis
Institutions: Massachusetts Institute of Technology
Programme: MIT Superfund Research
Public web page: Not specified
Submitter: Dikshant Pradhan
Studies: Benthic fluxes of fluorescent dissolved organic material, salt and heat ..., CometChip Enables Parallel Analysis of Multiple DNA Repair Activities, Excision of mutagenic replication-blocking lesions suppresses cancer but..., Interaction of N-Nitroamines with Bincuelar Copper Complexs for Luminsec..., Molecular origins of mutational spectra produced by the environmental ca..., Novel In Vivo CometChip Reveals NDMA-Induced DNA Damage and Repair in Mu...
Assays: Absorption and Emission Spectroscopy - Data Linked, Absorption and Emission Spectroscopy Analysis - Data Linked, Chemical Challenge - Metadata, Chemical Synthesis - Metadata, Comet Chip - Data Linked, Comet Chip Analysis - Data Attached, Comet Chip Analysis - Data Attached, Crystallography - Data Linked, Electron Paramagnetic Resonance - Data Linked, Extraction and Library Creation - Metadata, Field Water Sensor Run, GPT Assay - Data Attached, GPT Assay – Data Attached, Genome Alignment - Data Linked, High Resolution Mass Spectra - Data Linked, High Resolution Mass Spectra Analysis - Data Linked, Illumina Sequencing - Data Linked, Mass Spectrometry Processing – Data Linked, Mass Spectrometry – Data Linked, Mouse Necropsy – Metadata, Mutational Spectral Analysis - Data Attached, Necropsy - Metadata, Nuclear Magnetic Resonance Spectroscopy - Data Linked, Nuclear Magnetic Resonance Spectroscopy Analysis - Data Linked, RaDR Image Machine Learning Analysis – Data Attached, Tissue Collection - Metadata, Tissue Imaging – Metadata, Tissue Lysis – Metadata, X-ray Photoelectron Spectroscopy - Data Linked
Norah Owiti, Joshua Corrigan, Lee Pribyl, Jennifer Kay, Bevin Engelward
https://doi.org/10.3390/ijms231911776
The comet assay is a versatile assay for detecting DNA damage in eukaryotic cells. The assay can measure the levels of various types of damage, including DNA strand breaks, abasic sites and alkali-sensitive sites. Furthermore, the assay can also be modified to include purified DNA glycosylases so that alkylated and oxidized bases can be detected. The ...
Submitter: Charles Demurjian
Investigation: MIT SRP
Assays: Chemical Challenge - Metadata, Comet Chip - Data Linked, Comet Chip Analysis - Data Attached, Tissue Collection - Metadata
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
Submitter: Charles Demurjian
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: MIT SRP
