Assays

255 Assays visible to you, out of a total of 466

Using PCA, three components, beam size 8.
Clustering via MCL from Biolayout Express 3D

Data is taken from "Genome-Wide Gene Expression Analysis of the Switch between Acidogenesis and Solventogenesis in Continuous Cultures of Clostridium acetobutylicum." Grimmler et al. 2011
DOI: 10.1159/000320973

Contributor: Sebastian Curth

Biological problem addressed: Gene Expression

Snapshots: No snapshots

Development of bioinformatic tools to investigate the role of transcription factors and 14-3-3 proteins in the regulation of genes involved in cation homeostasis.

Contributor: Falko Krause

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

No description specified

Contributor: Dawie Van Niekerk

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Inhibition of lactate flux due to glucose transport inhibitor

No description specified

Contributor: Falko Krause

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

No description specified

Contributor: Sebastian Curth

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Elucidation of protein networks involved in the regulation of cation homeostasis using protein interaction datasets.

Contributor: Falko Krause

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

To estimate the differnces of internal pH between the wild type, single trk1 and trk2 or double trk1 trk2 mutants, the pH-dependent variant of GFP pHluorin was expressed in cells from a multicopy plasmid, cells were grown under various conditions, and the cell fluorescence was monitored.

Contributor: Falko Krause

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei exposed to methylene blue have been absolutely quantified using isotope ratio based MS technique.

26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei under pH stress (pH8.7) have been absolutely quantified using isotope ratio based MS technique.

intracellular metabolite measured by GC/MS and LC/MS

Contributor: Hanna Meyer

Assay type: Metabolomics

Technology type: NMR

Snapshots: No snapshots

No description specified

Pilot experiment concerning metabolome of S. solfataricus was conducted in order to acquire SOPs regarding the technique and gain insight on differences in metabolite concentrations at 70 and 80C

Contributor: Pawel Sierocinski

Assay type: Metabolomics

Technology type: Gas Chromatography Mass Spectrometry

Snapshots: No snapshots

We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
SigmaB itself is placed downstream of Pspac, inducible by IPTG. The lacZ reporter gene is downstream of Pctc promoter.
IPTG concentrations of 0.1, 0.2 and 1 mM are added in mid-exponential phase at an OD of appr. 0.3. The whole experiment runs for about eight hours.

We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.

There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.

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