Assays

240 Assays visible to you, out of a total of 446
No description specified

Contributor: Dawie Van Niekerk

Biological problem addressed: Modelling Analysis

Investigation: Glucose metabolism in Plasmodium falciparum tro...

Study: Model construction

No description specified

Extracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.

Intracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.

Kinetic characterisation and mathematical modelling of LDH.

Contributor: Dawie Van Niekerk

Biological problem addressed: Modelling Analysis

Investigation: Glucose metabolism in Plasmodium falciparum tro...

Study: Model construction

No description specified

Km values of pyruvate kinase of different organisms without/with allosteric effector molecules collected from literature.

Contributor: Stefan Henrich

Assay type: Experimental Assay

Technology type: Technology type

Investigation: The Attic

Study: allosteric regulation of pyruvate kinase

1. Preparation of B. subtilis cultures

Inoculate cells from -80°C stocks in 10 ml time-lapse microscopy (TLM) medium (62 mM K2HPO4 , 44mM KH2PO4, 15 mM (NH4)2SO4, 6.5 mM sodium citrate, 0.8 mM MgSO4, 0.02 % casamino acids, 27.8 mM glucose, 0.1 mM L-tryptophan, the pH was set to 7 using a KOH solution) supplemented with antibiotics, if necessary.
Grow the cells overnight in a shake flask (30°C, 225 rpm).
The following morning, dilute the cells 1:10 in pre-warmed chemically defined medium (CDM) (62
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