Activity of TbTryS measured by HPLC
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TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of GSH, and Spd.

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Created: 6th Aug 2014 at 16:12

Last updated: 8th Aug 2014 at 13:44

Last used: 23rd Jan 2019 at 16:26

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