Assay type 'Proteomics'



Related assays
19 Assays visible to you, out of a total of 35Contributor: Sandra Maass
Assay type: Proteomics
Technology type: 2D Gel Electrophoresis
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Contributor: Sandra Maass
Assay type: Protein Expression Profiling
Technology type: Mass Spectrometry
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Contributor: Sandra Maass
Assay type: Proteomics
Technology type: Mass Spectrometry
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Samples obtained form the central fermentation facility of Sulfosys have been compared using iTRAQ (isobaric tag for relative and absolute quantification). A pilot experiment resulted in creation of SOP and initial data on cells grown at 70 and 80C
Contributor: Pawel Sierocinski
Assay type: Protein Expression Profiling
Technology type: Isotope Ratio Mass Spectrometry
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Some examples of proteomics templates for Mass Spectrometry data that conform to the MIAPE specification
Contributor: Katy Wolstencroft
Assay type: Proteomics
Technology type: Mass Spectrometry
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We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
SigmaB itself is placed downstream of Pspac, inducible by IPTG. The lacZ reporter gene is downstream of Pctc promoter.
IPTG concentrations of 0.1, 0.2 and 1 mM are added in mid-exponential phase at an OD of appr. 0.3. The whole experiment runs for about eight hours.
Contributor: Ulf Liebal
Assay type: Proteomics
Technology type: Technology Type
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Contributor: Jay Moore
Assay type: Proteomics
Technology type: Gas Chromatography Mass Spectrometry
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Investigation of all steady state pH-values between pH 5.7 and 4.5 (pH 5.5, 5.3, 5.1, 4.9, 4.7).
Contributor: Sara Jabbari
Assay type: Proteomics
Technology type: Technology Type
Snapshots: No snapshots
Contributor: Sandra Maass
Assay type: Protein Expression Profiling
Technology type: Mass Spectrometry
Snapshots: No snapshots
Contributor: Sandra Maass
Assay type: Proteomics
Technology type: Mass Spectrometry
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For analyzing the binding of CcpA-HPrSer46P-complexes to various cre-elements, Surface Plasmon Resonance was used. All operations were carried out on a Biacore X instrument (Biacore, Uppsala, Sweden). Biotinylated cre DNA was coupled on a Neutravidin coated chip in flowcell two, a biotinylated reference DNA in flowcell one. For visualizing only the interactions of the CcpA-HPrSer46P-complex with the cre elements, CcpA was saturated with 50µM HPrSer46P. Titrations were carried out with 5-100nM
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Contributor: Maike Bartholomae
Assay type: Protein-protein Interaction
Technology type: Surface Plasmon Resonance
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Absolute quantification of proteins using heavy labeled QconCAT as an internal standard and quantifying the native proteins in the complex sample via scheduled Multiple Reaction Monitoring(MRM) .
Contributor: Praveen Kumar Sappa
Assay type: Proteomics
Technology type: Liquid Chromatography Mass Spectrometry
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Examples of proteomics templates for gele electrophoresis data that conform to the MIAPE-GE specification
Contributor: Katy Wolstencroft
Assay type: Proteomics
Technology type: Electrophoresis
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Contributor: Theresa Kouril
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
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Contributor: Jacqueline Wolf
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
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Plant material
The same plant material used for transcriptome analysis in (Flis et al., 2016) was the basis of our proteome study. Briefly, Arabidopsis thaliana Col-0 plants were grown on GS 90 soil mixed in a ratio 2:1 (v/v) with vermiculite. Plants were grown for 1 week in a 16 h light (250 μmol m−2 s−1, 20 °C)/8 h dark (6 °C) regime followed by an 8 h light (160 μmol m−2 s−1, 20 °C)/16 h dark (16 °C) regime for one week. Plants were then replanted with five seedlings per pot, transferred for
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Contributor: Daniel Seaton
Assay type: Protein Quantification
Technology type: Mass Spectrometry
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Proteomics data for N15 incorporation into protein in Ostreococcus grown in 12L:12D light:dark cycles.
Contributor: Daniel Seaton
Assay type: Proteomics
Technology type: Mass Spectrometry
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Quantitative proteomic analysis of Cyanothece ATCC51142 grown in 12L:12D light:dark cycles, using partial metabolic labeling and LC-MS analysis.
Contributor: Daniel Seaton
Assay type: Proteomics
Technology type: Mass Spectrometry
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Contributor: Ziva Ramsak
Assay type: Protein Expression Profiling
Technology type: Orbitrap Velos
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