Binding constants for CcpA with HPrSer46P interacting with various cre-elements

For analyzing the binding of CcpA-HPrSer46P-complexes to various cre-elements, Surface Plasmon Resonance was used. All operations were carried out on a Biacore X instrument (Biacore, Uppsala, Sweden). Biotinylated cre DNA was coupled on a Neutravidin coated chip in flowcell two, a biotinylated reference DNA in flowcell one. For visualizing only the interactions of the CcpA-HPrSer46P-complex with the cre elements, CcpA was saturated with 50µM HPrSer46P. Titrations were carried out with 5-100nM CcpA. The kinetic parameters were calculated with the BIAEVALUATION software 3.1 from the sensorgrams.

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Created: 7th Mar 2011 at 14:32

Last updated: 8th Nov 2017 at 15:21

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