For analyzing the binding of CcpA-HPrSer46P-complexes to various cre-elements, Surface Plasmon Resonance was used. All operations were carried out on a Biacore X instrument (Biacore, Uppsala, Sweden). Biotinylated cre DNA was coupled on a Neutravidin coated chip in flowcell two, a biotinylated reference DNA in flowcell one. For visualizing only the interactions of the CcpA-HPrSer46P-complex with the cre elements, CcpA was saturated with 50µM HPrSer46P. Titrations were carried out with 5-100nM CcpA. The kinetic parameters were calculated with the BIAEVALUATION software 3.1 from the sensorgrams.
SEEK ID: https://fairdomhub.org/assays/135
Investigation: A dynamic model of the CcpA regulation network
Assay type: Protein-protein Interaction
Technology type: Surface Plasmon Resonance
Organisms: No organisms
Created: 7th Mar 2011 at 14:32
Last updated: 8th Nov 2017 at 15:21