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Created: 28th Apr 2010 at 18:21
Last updated: 19th Oct 2012 at 17:57
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.
Contributor: Ulf Liebal
Biological problem addressed: Gene expression
Investigation: The transition from growing to non-growing Baci...
Study: Controlled sigmaB induction in shake flask
Organisms: Bacillus subtilis : 168 (wild-type / wild-type) (batch)
Models: BSA115 Post-transcriptional instability, BSA115-lacz-synth, BSA115-sigB-adaptation, BSA115-transcription inhibition model
SOPs: No SOPs
Data files: 20090915_BSA115-IPTG-assay, Model-representations-BSA115-Experiments and 1 hidden item
Absolute quantification of proteins using heavy labeled QconCAT as an internal standard and quantifying the native proteins in the complex sample via scheduled Multiple Reaction Monitoring(MRM) .
Contributor: Praveen Kumar Sappa
Assay type: Proteomics
Technology type: Liquid chromatography mass spectrometry
Investigation: Multiomics study of Bacillus subtilis under osm...
Study: B. subtilis_SysMo2_Chemostat_growthrate-salt
Organisms: Bacillus subtilis : 168 (wild-type / wild-type) (chemostat)
Data files: 1 hidden item
Theoretical analysis of hypothetical sigma factor competition.
Based on the model 'transcription factor competition' possible dynamics of sigma factor competition are simulated and analysed using Lineweaver-Burk representations.
Study: B. subtilis Transcription Factor Competition
Organisms: Bacillus subtilis : 168 (wild-type / wild-type)
Models: No Models
Data files: B. subtilis Transcription Factor Competition - ...