SEEK ID: https://fairdomhub.org/assays/275
Projects: SulfoSys - Biotec
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
Assay type: Proteomics
Technology type: Tandem Mass Spectrometry
Organisms: Sulfolobus solfataricus (batch)
Created: 5th Feb 2015 at 13:42
Last updated: 8th Nov 2017 at 15:21
I am the foundation Professor of Systems Biology and Engineering within the Department of Chemical and Process Engineering (CPE), at The University of Sheffield. My research philosophy is centred on a mechanistic systems biology approach to solve biochemical reaction engineered processes. I wish to pursue issues involved in the effective utilisation of biological resources. The approach is specifically targeted at the conjunction of chemical engineering (metabolic engineering and synthetic biology),
Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012)
Integrated systems biology approach including transcriptome, metabolome, biochemistry, proteome analyses and modelling to elucidate the catabolic pathway for L-fucose in S. solfataricus P2.
Snapshots: No snapshots
Person responsible: Theresa Kouril
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Comparative proteomics of S. solfatricus grown on either L-fucose or D-glucose.
Contributor: Jacqueline Wolf
Relationship type: Not specified
Investigations: L-fucose degradation in Sulfolobus solfataricus P2
Assays: Proteome analysis: d-fuc / l-glu
Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
Authors: Jacqueline Wolf, Helge Stark, K. Fafenrot, Andreas Albersmeier, Trong Khoa Pham, K. B. Muller, B. Meyer, L. Hoffmann, L. Shen, Stefan Albaum, Theresa Kouril, K. Schmidt-Hohagen, M. Neumann-Schaal, C. Brasen, J. Kalinowski, Phil Wright, Sonja-Verena Albers, Dietmar Schomburg, Bettina Siebers
Date Published: 10th Sep 2016
Journal: Mol Microbiol
PubMed ID: 27611014
Citation: Mol Microbiol. 2016 Sep 9. doi: 10.1111/mmi.13498.