SULFOSYSbiotech_Proteome analysis
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View contentProtein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis
FAIRDOMHub ID: https://seek.sysmo-db.org/sops/211?version=1
Filename: SOP_Proteomics_SEEK.pdf
Format: PDF document
Size: 171 KB
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Views: 491 Downloads: 13
Created: 5th Feb 2015 at 13:45
Last updated: 20th Oct 2016 at 08:23
Last used: 25th Sep 2017 at 04:48
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Related items
Projects: SulfoSys, SulfoSys - Biotec
Institutions: University Duisburg-Essen
Projects: SulfoSys, SulfoSys - Biotec
Institutions: University of Sheffield
Projects: SulfoSys, SulfoSys - Biotec
Institutions: University of Sheffield
I am the foundation Professor of Systems Biology and Engineering within the Department of Chemical and Process Engineering (CPE), at The University of Sheffield. My research philosophy is centred on a mechanistic systems biology approach to solve biochemical reaction engineered processes. I wish to pursue issues involved in the effective utilisation of biological resources. The approach is specifically targeted at the conjunction of chemical engineering (metabolic engineering and synthetic biology),
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Within the e:Bio - Innovationswettbewerb Systembiologie (Federal Ministry of Education and Research (BMBF)), the SulfoSYSBIOTECH consortium (10 partners), aim to unravel the complexity and regulation of the carbon metabolic network of the thermoacidophilic archaeon Sulfolobus solfataricus (optimal growth at 80°C and pH 3) in order to provide new catalysts ‘extremozymes’ for utilization in White Biotechnology.
Based on the available S. solfataricus genome scale metabolic model (Ulas et al., 2012)
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Programme: e:Bio
Public web page: http://www.sulfosys.com/
Integrated systems biology approach including transcriptome, metabolome, biochemistry, proteome analyses and modelling to elucidate the catabolic pathway for L-fucose in S. solfataricus P2.
Studies: Comparison of S. solfataricus grown on l-fucose and d-glucose
Person responsible: Theresa Kouril
Assays: Cell free extract activity measurements: L-fuc/d-glc, Metabolic model of Sulfolobus solfataricus, Proteome analysis: d-fuc / l-glu, RNA sequencing:l-fuc/d-glu, intracellular metabolome analysis: l-fucose vs d-glucose
Contributor: Theresa Kouril
Assay type: Proteomics
Technology type: Tandem mass spectrometry
Investigation: L-fucose degradation in Sulfolobus solfataricus P2
