IPTG induction of sigmaB in BSA115

We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
SigmaB itself is placed downstream of Pspac, inducible by IPTG. The lacZ reporter gene is downstream of Pctc promoter.
IPTG concentrations of 0.1, 0.2 and 1 mM are added in mid-exponential phase at an OD of appr. 0.3. The whole experiment runs for about eight hours.

FAIRDOMHub ID: https://seek.sysmo-db.org/assays/21

Experimental Assay

Ulf Liebal

Projects: BaCell-SysMO

Investigation: The transition from growing to non-growing Bacillus subtilis cells

Study: Controlled sigmab induction in shake flask

Assay type: Proteomics

Technology type: Technology type

Organisms: Bacillus subtilis (batch)

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Views: 987

Created: 16th Nov 2009 at 16:10

Last updated: 20th May 2014 at 16:22

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