SOPs
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Creator: Martijn Bekker
Submitter: The JERM Harvester
This assay uses a dual-wavelength spectrophotometer to quantify cytochromes present in the E. coli respiratory chain.
Creators: Alison Graham, Robert Poole, Jeff Green
Submitter: Alison Graham
Quantitative real-time PRC is used to compare kdpFABC expression between the E. coli strains MG1655 (wildtype)and MG1655 (kdpA4, a kdpFABC-inactive mutant after a shift to K+ limitation.
Creator: Lisbeth Lyngberg
Submitter: Lisbeth Lyngberg
Method extraction of intracellular metabolites in Lactococcus lactis
Creator: Martijn Bekker
Submitter: The JERM Harvester
This method describes how one can quench metabolism of Escherichia coli and extract metabolites from many kinds of metabolite classes like: nucleotides, sugar-phosphates, organic acids ....
Creator: Stefan Stagge
Submitter: Stefan Stagge
A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.
Creator: Martijn Bekker
Submitter: Martijn Bekker