Creator: Martijn Bekker
This assay uses a dual-wavelength spectrophotometer to quantify cytochromes present in the E. coli respiratory chain.
Creators: Alison Graham, Robert Poole, Jeff Green
Contributor: Alison Graham
Quantitative real-time PRC is used to compare kdpFABC expression between the E. coli strains MG1655 (wildtype)and MG1655 (kdpA4, a kdpFABC-inactive mutant after a shift to K+ limitation.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Method extraction of intracellular metabolites in Lactococcus lactis
This method describes how one can quench metabolism of Escherichia coli and extract metabolites from many kinds of metabolite classes like: nucleotides, sugar-phosphates, organic acids ....
Creator: Stefan Stagge
Contributor: Stefan Stagge
A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.
Contributor: Martijn Bekker