SOPs

149 SOPs visible to you, out of a total of 243

Sample preparation procedure for metabolic analysis on T. b. brucei 427 using LC/MS

Creator: Dong-Hyun Kim

Contributor: Dong-Hyun Kim

For the study of mRNA decay rates, transcription was inhibited with ActinomycinD, and RNA splicing with Sinefungin, at different time points, in the Matthews lab. rRNA depleted RNA was extracted from each of the samples in the Clayton lab, and sent for deep sequencing at the BioQuant facility in Heidelberg

Creator: Federico Rojas

Contributor: Federico Rojas

Sample preparation procedure for metabolic footprint analysis on T. b. brucei 427 using LC/MS

Creator: Dong-Hyun Kim

Contributor: Dong-Hyun Kim

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

Biomass sampling - SOP for sampling of biomass

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

No description specified

Creator: Michael Kohlstedt

Contributor: Michael Kohlstedt

This is a well-established, classical genetic method of constructing chromosomal monolysogenic fusions to a promoterless lacZ gene.

Creators: Alison Graham, Jeff Green, Robert Poole

Contributor: Alison Graham

No description specified

Creator: Martijn Bekker

Contributor: JERM

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

The reverse transcriptase synthesizes DNA, which complements the mRNA template
(complementary DNA, cDNA). Cy3/Cy5-dCTP are incorporated into cDNA during Reverse transcription. The obtained Cy3/Cy5 cDNA are then competitively hybridised onto Agilent microarray slide and subsequently scanned.

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

SOP for shake flask cultivation of B.Subtilis in Bacell-Sysmo

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

ClosTron mutants should always be subjected to Southern blot analysis to ensure that only
one intron insertion has occurred.

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

No description specified

Creators: Holger Janssen, Tomas Fiedler

Contributor: Holger Janssen

SOPs related with medium composition, fermentation and stocking of S. solfataricus.

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

Standard procedure regarding intracellular metabolome analysis using GC-MS.

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

Protocol for RNA isolation, cDNA synthesis and labeling and hybridization and cleanup of Sulfolobus solfataricus microarray

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

Standard operating procedures regarding iTraq based proteomics.

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

No description specified

Creator: Theresa Kouril

Contributor: Theresa Kouril

Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis

Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham

Contributor: Theresa Kouril

No description specified

Creators: Theresa Kouril, Andreas Albersmeier, CeBiTech, University Bielefeld, Germany; Jörn Kalinowski, CeBiTech, University Bielefeld;

Contributor: Theresa Kouril

This SOP describes the SUMO procedure for determining B-galactosidase activities.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.

Creator: Sonja Steinsiek

Contributor: Sonja Steinsiek

This file describes how to isolate mRNA from E. coli using the kit from Epicentre, for gene expression analysis via RT-PCR

Creator: Sonja Steinsiek

Contributor: Sonja Steinsiek

This SOP describes the preparation of E. coli RNA

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

This protocol describes the transcriptional profiling of E. coli cultures using microarrays. The protocol utilises RNA isolated as described in another SOP (SUMO RNA isolation from E. coli) and with hybridisation to Ocimum Ocichip E. coli K-12 microarrays.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

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