SOPs

149 SOPs visible to you, out of a total of 243

A kinetic model that describess the activation of a dimeric efflux system that could bind either GSH or SLG

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A model to describe aggregation of homomeric protein complexes in mechanosensitive channels in E.coli.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

SOP for measurement of ALD activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for growing yeast in anaerobic conditions

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

This HPLC method uses a isocratic method and a RI detector to identify and quantify almost all excreted catabolic metabolites.

Creator: Martijn Bekker

Contributor: Martijn Bekker

Method for analysis of various organic acids in the medium

Creator: Martijn Bekker

Contributor: JERM

Measurements of K+ retained in the cytoplasm using flame photometry.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A procedure to analyse the genomic interaction of E.coli RNA polymerase (RNAP) upon methylglyoxal (MG) stress, a toxic keto-aldehyde by-product of metabolism.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Assay methodologies for individual glycolytic isoenzymes from the Mendes Group, University of Manchester, UK

Creator: Hanan Messiha

Contributor: Walter Glaser

The kit is used for the quantitative detection of ATP in yeast cells by luciferase driven
bioluminescence. ATP extraction is done by boiling cells in TE buffer.

Creators: Martin Valachovic, Cornelia Klein

Contributor: Walter Glaser

Protocol for transfer of plasmids into Clostridium
acetobutylicum ATCC 824 by electroporation

Creators: None

Contributor: Ying Zhang

Purpose: Cation content analysis - protocol

Creator: Clara Navarrete

Contributor: JERM

The reverse transcriptase synthesizes DNA, which complements the mRNA template.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

Cloning and heterologous expression of gluconeogenic enzymes from S. solfataricus in E. coli

Creators: Jacky Snoep, Theresa Kouril

Contributor: Jacky Snoep

A refined and streamlined procedure to generate mutant in a wide range of different clostridial species, using group II intron retargeting methodologies.

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

To induce kdpFABC expression, cells are cultivated in K10 minimal medium (10mM K+) were shifted into K+ limiting growth medium (K0), containing 20 μM K+ by filtration.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

SOP for the cultivation conditions of Plasmodium falciparum, including the protocol for synchronisation.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

No description specified

Creator: Martijn Bekker

Contributor: JERM

This method describes how to derivatize the N-glutathionylspermidine and trypanothione produced by T. brucei trypanothione synthetase under in vivo-like conditions

Creator: Alejandro Leroux

Contributor: Alejandro Leroux

Sample preparation - SOP for sampling, preparation of cell-free extracts, and determination of total extracted protein

Creator: Femke Mensonides

Contributor: Maksim Zakhartsev

Sample preparation - SOP for sampling, preparation of cell extracts, and general assay set-up

Creator: Femke Mensonides

Contributor: Maksim Zakhartsev

The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

General protocol for measuring the kinetic parameters of the purified glycolytic enzymes from Saccharomyces cerevisiae - SOP for measuring the kinetic parameters of the purified glycolytic isoenzymes

Creator: Hanan Messiha

Contributor: Maksim Zakhartsev

SOP for measurement of ENO activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

No description specified

Creator: Katja Bettenbrock

Contributor: JERM

Extraction and quantitative analysis of proteins from FFPE tissue

Creators: Olga Krebs, Ruben Van Heck

Contributor: Olga Krebs

A Standard Operating Procedure (SOP) is a document consisting of step-by-step information on how to execute a task. An existing SOP may need to just be modified and updated.

Creator: Olga Krebs

Contributor: JERM

No description specified

Creator: John Raedts

Contributor: John Raedts

Experimental system that is designed to observe the in vivo stability and aggregation of a protein of interest.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

The procedure describes the preparation of fluorescent DNA probes from human mRNA or total
RNA.

Creator: Olga Krebs

Contributor: Olga Krebs

SOP for measurement of G3PDH activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of GAPDH activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

The Gene-doctoring method of lambda-red deletion (Lee et al., 2009) was modified slightly to create chromosomal mutations of fnr.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

Generating gene knock-ins using MJF618 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A protocol to improve conventional, recombination-based gene knock-out methodologies thtough the provision of negative selection markers, pyrE or codA.

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

Generating gene knock-outs using DY330 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Protocol for transfer of plasmids into
Clostridium spp. by conjugation

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

A model for translation elongation, which allows the prediction of how different conditions and parameters affect the rate and throughput of translation.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

This is the general protocol for the glycolytic enzyme measurements. Detailed Information for each Enzyme can be found in the SOP: Assays for measuring the activities of the individual glycolytic isoenzymes of Saccharomyces cerevisiae

Creator: Hanan Messiha

Contributor: Walter Glaser

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

We routinely select specific RNAi gene targets (400–600 bp) and primers using the RNAit software http://trypanofan.path.cam.ac.uk/software/RNAit.html. A single pair of PCR primers are designed that incorporate four selected restriction sites (not present in the RNAi target fragment) such that a single PCR product can be differentially digested and sequentially cloned. For example, using MCS1/2, the following primers could be used to clone antisense followed by sense fragments: Primer 1, XbaI–BamHI-5′
...

Creator: Federico Rojas

Contributor: Federico Rojas

Labelling and extraction procedure for uniformly 13C-labelled E. coli (MG1655) for absolute quantification using isotope dilution technique by LC-MS

Creator: Dong-Hyun Kim

Contributor: Dong-Hyun Kim

SOP for measurement of glucose transport activity in intact trophozoites.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP used for detecting non influential parameters and interactions in non linear dynamical models. These parameters can be estabilished which allows the prioritization of parameters that can be subsequently estimated using robust global optimizations methods.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.

Creator: Martijn Bekker

Contributor: Martijn Bekker

Protocol for applying a glucose perturbation in Streptococcus pyogenes.

Creator: Martijn Bekker

Contributor: Martijn Bekker

MIAPE (Minimum Information About a Proteomics Experiment) is the recommended format for proteomics data in SysMO-SEEK. The document attached provides more information and links to tools and resources.

Creator: Katy Wolstencroft

Contributor: Katy Wolstencroft

Written Standard Operating Procedures provide workers with the operational
information necessary to perform a job properly and ensure consistency in the
operations. Standard Operating Procedures provide a historical record of steps in
the how, why and when and serve as a training tool for teaching users.

Creator: Olga Krebs

Contributor: Olga Krebs

SOP for measurement of HK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

This method describes the preparation of the in vivo-like buffer for the measurement of bloodstream T. brucei recombinant enzymes under pseudo-physiological conditions.

Creators: Alejandro Leroux, Luise Krauth-Siegel

Contributor: Alejandro Leroux

No description specified

Creator: Federico Rojas

Contributor: Federico Rojas

This protocol is designed to prepare sufficient amounts of high-quality total
RNA from the yeast saccharomyces cerevisiae grown in liquid culture for
analysis on microarrays.

Creators: Walter Glaser, Christa Gregori

Contributor: Walter Glaser

Method for synthesis of LAB-medium sued for the SYSMO-LAB project

Creator: Martijn Bekker

Contributor: JERM

SOP for measurement of LDH activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

No description specified

Creator: Sonja Steinsiek

Contributor: JERM

A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Measurement of the transmembrane pH gradient and thus pHi, when the external pH is known.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creator: Tomas Fiedler

Contributor: Tomas Fiedler

No description specified

Creator: Tomas Fiedler

Contributor: Tomas Fiedler

FRAP experiments are used for studying cytoplasmic diffusion in cells and cells membrane.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A model to describe the effect of K+ uptake by KdpFABC on the two-component system KdpD/KdpE, which enables the identification of control principles of the Kdp system

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Mathematical model of pH buffering, which allows the prediction of how the buffering capacity depends on the cytoplasm's composition, for any number of buffers.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Pulsed-FRAP measure the diffusion constants in confined volumes in small cells like E. coli and other bacteria or cellular organelles.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

SOP for extracellular metabolite measurment

Creator: Hanna Meyer

Contributor: Hanna Meyer

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for OptKnock.

Creator: Natalie Stanford

Contributor: Natalie Stanford

Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

SOP for measurement of PFK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGI activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGM activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGM activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

No description specified

Creator: Lina Patricia Barreto Parra

Contributor: Lina Patricia Barreto Parra

SOP for measurement of PK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

Method for transformation of plasmids into Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

Purpose:

Creator: Jose Ramos

Contributor: JERM

Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.

Creators: Jacky Snoep, Theresa Kouril

Contributor: Jacky Snoep

The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins

Creator: Miguel Curto Rubio

Contributor: JERM

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins

Creator: Miguel Curto Rubio

Contributor: JERM

Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts

Creators: Jacky Snoep, Theresa Kouril

Contributor: Jacky Snoep

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

Creator: Martijn Bekker

Contributor: Martijn Bekker

Method of how to perform the purification of glyoxalase II as well as kinetics assays.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A method of how to measure methylglyoxal present in the growth medium.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creator: Martijn Bekker

Contributor: JERM

This assay uses a dual-wavelength spectrophotometer to quantify cytochromes present in the E. coli respiratory chain.

Creators: Alison Graham, Robert Poole, Jeff Green

Contributor: Alison Graham

Quantitative real-time PRC is used to compare kdpFABC expression between the E. coli strains MG1655 (wildtype)and MG1655 (kdpA4, a kdpFABC-inactive mutant after a shift to K+ limitation.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

This method describes how one can quench metabolism of Escherichia coli and extract metabolites from many kinds of metabolite classes like: nucleotides, sugar-phosphates, organic acids ....

Creator: Stefan Stagge

Contributor: Stefan Stagge

A protocol for acidic quenching of lactic acid bacteria used for analyses of intracellular metabolites.

Creator: Martijn Bekker

Contributor: Martijn Bekker

Metabolic networks with gene expression are researched under very different banners with different techniques. For example, there are the dynamic enzyme-cost Flux Balance Analysis (deFBA) [1], conditional Flux Balance Analysis [2], Metabolism and Expression models (ME models) [3], Resource Balance Analysis [4], etc. At their core, these methods can all understood as Resource Allocation Models (RAM) and while investigating their potential and their results, we encountered the problem of sharing
...

Creators: Henning Lindhorst, Alexandra-M. Reimers

Contributor: Henning Lindhorst

A method to compare kdpFABC expression between MG1655 (wildtype) and MG1655 (kdpA4) after a shift to K+ limitation, the RNA was extracted from samples taken at different points in time.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Cells were harvested from culture keeping the cells cold to quench the physiological condition of RNA and the cells were mechanically disrupted. RNA was isolated from the cells by conventional acid-phenol method and the quality was checked by Agilent bioanalyser.

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

No description specified

Creator: Holger Janssen

Contributor: JERM

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236).

Creator: Natalie Stanford

Contributor: Natalie Stanford

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for RobustKnock.

Creator: Natalie Stanford

Contributor: Natalie Stanford

SOP for ß-Galactosidase assay.

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

Sample preparation procedure for metabolic analysis on T. b. brucei 427 using LC/MS

Creator: Dong-Hyun Kim

Contributor: Dong-Hyun Kim

For the study of mRNA decay rates, transcription was inhibited with ActinomycinD, and RNA splicing with Sinefungin, at different time points, in the Matthews lab. rRNA depleted RNA was extracted from each of the samples in the Clayton lab, and sent for deep sequencing at the BioQuant facility in Heidelberg

Creator: Federico Rojas

Contributor: Federico Rojas

Sample preparation procedure for metabolic footprint analysis on T. b. brucei 427 using LC/MS

Creator: Dong-Hyun Kim

Contributor: Dong-Hyun Kim

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

Biomass sampling - SOP for sampling of biomass

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

No description specified

Creator: Michael Kohlstedt

Contributor: Michael Kohlstedt

This is a well-established, classical genetic method of constructing chromosomal monolysogenic fusions to a promoterless lacZ gene.

Creators: Alison Graham, Jeff Green, Robert Poole

Contributor: Alison Graham

No description specified

Creator: Martijn Bekker

Contributor: JERM

Method extraction of intracellular metabolites in Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

No description specified

Creator: Martijn Bekker

Contributor: JERM

The reverse transcriptase synthesizes DNA, which complements the mRNA template
(complementary DNA, cDNA). Cy3/Cy5-dCTP are incorporated into cDNA during Reverse transcription. The obtained Cy3/Cy5 cDNA are then competitively hybridised onto Agilent microarray slide and subsequently scanned.

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

SOP for shake flask cultivation of B.Subtilis in Bacell-Sysmo

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

ClosTron mutants should always be subjected to Southern blot analysis to ensure that only
one intron insertion has occurred.

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

No description specified

Creators: Holger Janssen, Tomas Fiedler

Contributor: Holger Janssen

SOPs related with medium composition, fermentation and stocking of S. solfataricus.

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

Standard procedure regarding intracellular metabolome analysis using GC-MS.

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

Protocol for RNA isolation, cDNA synthesis and labeling and hybridization and cleanup of Sulfolobus solfataricus microarray

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

Standard operating procedures regarding iTraq based proteomics.

Creator: Pawel Sierocinski

Contributor: Pawel Sierocinski

No description specified

Creator: Theresa Kouril

Contributor: Theresa Kouril

Protein extraction, iTRAQ labeling, peptides separation, mass spectrometry and data analysis

Creators: Theresa Kouril, Phil Wright, Trong Khoa Pham

Contributor: Theresa Kouril

No description specified

Creators: Theresa Kouril, Andreas Albersmeier, CeBiTech, University Bielefeld, Germany; Jörn Kalinowski, CeBiTech, University Bielefeld;

Contributor: Theresa Kouril

This SOP describes the SUMO procedure for determining B-galactosidase activities.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

This document describes the chemostat conditions to run comparable experiments in different bioreactors in different labs.

Creator: Sonja Steinsiek

Contributor: Sonja Steinsiek

This file describes how to isolate mRNA from E. coli using the kit from Epicentre, for gene expression analysis via RT-PCR

Creator: Sonja Steinsiek

Contributor: Sonja Steinsiek

This SOP describes the preparation of E. coli RNA

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

This protocol describes the transcriptional profiling of E. coli cultures using microarrays. The protocol utilises RNA isolated as described in another SOP (SUMO RNA isolation from E. coli) and with hybridisation to Ocimum Ocichip E. coli K-12 microarrays.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

No description specified

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

The model describes the series of chemical reactions in MG detoxification pathway, allowing one to predict the flux of all compounds produced during detoxification.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

Isolation of total RNA from Bacillus Subtilis using phenol-chloroform extraction method by maintaining cryogenec conditions initailly to prevent RNA degradation. Quality of the obtained RNA is then tested with Agilent Bioanalyser before proceeding for gene expression analysis.

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

SOP for measurement of TPI activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

An overview of creating MAGE-TAB compliant spreadsheets for transcriptomics data in SysMO SEEK

Creator: Katy Wolstencroft

Contributor: Katy Wolstencroft

SOP for the isolation of intact Plasmodium falciparum trophozoites from infected red blood cells and the preparation of a cell free extract that can be used for kinetic analyses.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

This is a protocol for determining the activity of the T. brucei Trypanothione synthetase under in vivo-like conditions.

Creators: Alejandro Leroux, Luise Krauth-Siegel

Contributor: Alejandro Leroux

SOP for the determination of external metabolites (Glc, Pyr, Gly, Lac) in intact trophozoite incubations, and for the determination of intracellular metabolite concentrations.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

This SOP defines the format of SOPs used in the SUMO consortium.

Creator: Michael Ederer

Contributor: Michael Ederer

Yeast strains used in the project

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

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