SOPs

153 SOPs visible to you, out of a total of 246

A procedure to measure the levels of GSH and SLG before and after exposure to MG using formic acid. A similar expreimental set up as for potassium efflux experiments is used to which a silicon oil centrifugation step is incorporated. Samples are analysed by LC-MS-MS in order to quantify intracellular GSH and SLG levels.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Measurement of the transmembrane pH gradient and thus pHi, when the external pH is known.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creator: Tomas Fiedler

Contributor: Tomas Fiedler

FRAP experiments are used for studying cytoplasmic diffusion in cells and cells membrane.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A model to describe the effect of K+ uptake by KdpFABC on the two-component system KdpD/KdpE, which enables the identification of control principles of the Kdp system

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Mathematical model of pH buffering, which allows the prediction of how the buffering capacity depends on the cytoplasm's composition, for any number of buffers.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Pulsed-FRAP measure the diffusion constants in confined volumes in small cells like E. coli and other bacteria or cellular organelles.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

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