SOPs

149 SOPs visible to you, out of a total of 243

SOP for measurement of G3PDH activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of GAPDH activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

The Gene-doctoring method of lambda-red deletion (Lee et al., 2009) was modified slightly to create chromosomal mutations of fnr.

Creator: Matthew Rolfe

Contributor: Matthew Rolfe

Generating gene knock-ins using MJF618 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A protocol to improve conventional, recombination-based gene knock-out methodologies thtough the provision of negative selection markers, pyrE or codA.

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

Generating gene knock-outs using DY330 expressing the defective lambdoid prophage recombination system λ red.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Protocol for transfer of plasmids into
Clostridium spp. by conjugation

Creators: Ying Zhang, Nigel Minton

Contributor: Ying Zhang

A model for translation elongation, which allows the prediction of how different conditions and parameters affect the rate and throughput of translation.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

This is the general protocol for the glycolytic enzyme measurements. Detailed Information for each Enzyme can be found in the SOP: Assays for measuring the activities of the individual glycolytic isoenzymes of Saccharomyces cerevisiae

Creator: Hanan Messiha

Contributor: Walter Glaser

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

We routinely select specific RNAi gene targets (400–600 bp) and primers using the RNAit software http://trypanofan.path.cam.ac.uk/software/RNAit.html. A single pair of PCR primers are designed that incorporate four selected restriction sites (not present in the RNAi target fragment) such that a single PCR product can be differentially digested and sequentially cloned. For example, using MCS1/2, the following primers could be used to clone antisense followed by sense fragments: Primer 1, XbaI–BamHI-5′
...

Creator: Federico Rojas

Contributor: Federico Rojas

Labelling and extraction procedure for uniformly 13C-labelled E. coli (MG1655) for absolute quantification using isotope dilution technique by LC-MS

Creator: Dong-Hyun Kim

Contributor: Dong-Hyun Kim

SOP for measurement of glucose transport activity in intact trophozoites.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP used for detecting non influential parameters and interactions in non linear dynamical models. These parameters can be estabilished which allows the prioritization of parameters that can be subsequently estimated using robust global optimizations methods.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Perturbation of starved cells with glucose. Concentrations of intra- and extracellular metabolites are followed in time.

Creator: Martijn Bekker

Contributor: Martijn Bekker

Protocol for applying a glucose perturbation in Streptococcus pyogenes.

Creator: Martijn Bekker

Contributor: Martijn Bekker

MIAPE (Minimum Information About a Proteomics Experiment) is the recommended format for proteomics data in SysMO-SEEK. The document attached provides more information and links to tools and resources.

Creator: Katy Wolstencroft

Contributor: Katy Wolstencroft

Written Standard Operating Procedures provide workers with the operational
information necessary to perform a job properly and ensure consistency in the
operations. Standard Operating Procedures provide a historical record of steps in
the how, why and when and serve as a training tool for teaching users.

Creator: Olga Krebs

Contributor: Olga Krebs

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