Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)
Creator: Maksim Zakhartsev
Contributor: Maksim Zakhartsev
SOP for measurement of PFK activity in extracts.
Creators: Dawie Van Niekerk, Jacky Snoep
Contributor: Dawie Van Niekerk
SOP for measurement of PGI activity in extracts.
SOP for measurement of PGK activity in extracts.
SOP for measurement of PGM activity in extracts.
Creator: Lina Patricia Barreto Parra
Contributor: Lina Patricia Barreto Parra
SOP for measurement of PK activity in extracts.
Method for transformation of plasmids into Lactococcus lactis
Creator: Martijn Bekker
Creator: Jose Ramos
Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.
Creators: Jacky Snoep, Theresa Kouril
Contributor: Jacky Snoep
The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.
Creator: Lisbeth Lyngberg
Contributor: Lisbeth Lyngberg
Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A
Contributor: Jay Moore
Creators: Louise Thomas, Maggie Smith
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins
Creator: Miguel Curto Rubio
Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins
Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts
This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis
Contributor: Martijn Bekker
Method of how to perform the purification of glyoxalase II as well as kinetics assays.
Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.
A method of how to measure methylglyoxal present in the growth medium.