SOPs

149 SOPs visible to you, out of a total of 243

Metabolic perturbations - SOP for metabolic perturbations (i.e. glucose pulse)

Creator: Maksim Zakhartsev

Contributor: Maksim Zakhartsev

SOP for measurement of PFK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGI activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGM activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

SOP for measurement of PGM activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

No description specified

Creator: Lina Patricia Barreto Parra

Contributor: Lina Patricia Barreto Parra

SOP for measurement of PK activity in extracts.

Creators: Dawie Van Niekerk, Jacky Snoep

Contributor: Dawie Van Niekerk

Method for transformation of plasmids into Lactococcus lactis

Creator: Martijn Bekker

Contributor: JERM

Purpose:

Creator: Jose Ramos

Contributor: JERM

Preparation of cell free extracts of the recombinant E. coli strains expressing the gluconeogenic S. solfataricus enzymes.

Creators: Jacky Snoep, Theresa Kouril

Contributor: Jacky Snoep

The fluorescent DNA-binding dye used in qRT-PCR binds to all kinds of double stranded DNA. To prevent false-positive results, the RNA is treated with DNase to remove remaining DNA.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

No description specified

Creators: Per Bruheim, Trond Ellingsen, Sunniva Hoel, Øyvind Jakobsen, Håvard Sletta, Alexander Wentzel, Anders Øverby, Strøm, A

Contributor: Jay Moore

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 1. Citosoluble proteins

Creator: Miguel Curto Rubio

Contributor: JERM

Purpose: PROTEIN EXTRACTION FOR 2-DE BASED PROTEOMIC EXPERIMENTS. 2. Membrane proteins

Creator: Miguel Curto Rubio

Contributor: JERM

Purification of gluconeogenic enzymes from S. solfataricus in recombinant E.coli extracts

Creators: Jacky Snoep, Theresa Kouril

Contributor: Jacky Snoep

No description specified

Creators: Louise Thomas, Maggie Smith

Contributor: Jay Moore

This protocol for applying glucose perturbations works for Lactococcus lactis and Enterococcus faecalis

Creator: Martijn Bekker

Contributor: Martijn Bekker

Method of how to perform the purification of glyoxalase II as well as kinetics assays.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Growing Escherichia coli to express KefF by adding IPTG for purification and kinetics experiments.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

A method of how to measure methylglyoxal present in the growth medium.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

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