SOPs

149 SOPs visible to you, out of a total of 243

Metabolic networks with gene expression are researched under very different banners with different techniques. For example, there are the dynamic enzyme-cost Flux Balance Analysis (deFBA) [1], conditional Flux Balance Analysis [2], Metabolism and Expression models (ME models) [3], Resource Balance Analysis [4], etc. At their core, these methods can all understood as Resource Allocation Models (RAM) and while investigating their potential and their results, we encountered the problem of sharing
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Creators: Henning Lindhorst, Alexandra-M. Reimers

Contributor: Henning Lindhorst

A method to compare kdpFABC expression between MG1655 (wildtype) and MG1655 (kdpA4) after a shift to K+ limitation, the RNA was extracted from samples taken at different points in time.

Creator: Lisbeth Lyngberg

Contributor: Lisbeth Lyngberg

Cells were harvested from culture keeping the cells cold to quench the physiological condition of RNA and the cells were mechanically disrupted. RNA was isolated from the cells by conventional acid-phenol method and the quality was checked by Agilent bioanalyser.

Creator: Praveen Kumar Sappa

Contributor: Praveen Kumar Sappa

No description specified

Creator: Holger Janssen

Contributor: JERM

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236).

Creator: Natalie Stanford

Contributor: Natalie Stanford

Matlab script that reproduces the model redesign results outlined in RobOKoD: microbial strain design for (over)production of target compounds (http://fairdomhub.org/publications/236) for RobustKnock.

Creator: Natalie Stanford

Contributor: Natalie Stanford

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