Studies

112 Studies visible to you, out of a total of 242

The pilot experiment has been set up in order to develop uniform SOPs for the Sulfosys consortium. It comprises proteomics, transcriptomics, metabolomics as well as enzymatic essays. Cells for all the members of the consortium have been obtained from the same batch fermentations according to fermentation SOP of Sulfosys. The pilot resulted in creating a procedures for all the techniques used in consortium.

Assays: Comparison of proteome of S. solfataricus at 70 and 80C, Comparison of transcriptome of S. solfataricus at 70 and 80C, Enzyme activity tests for S. solfataricus, Fermentation of S. solfataricus at 70 and 80C in a batch fermenter, Intracellular metabolomics of S. solfataricus at 70 and 80C

No description specified

Assays: Insilico Promotor Anaysis

Does the general proteome of yeast cells change in a strain lacking Trk1,2 transporter? Under which conditions?

Assays: 2D Gel Analysis

What is the proteome of starved cells. Main characteristics?

Person responsible: Falko Krause

Assays: 2D-Gel Electrophoresis, Protein Identification - MS

Pyruvate formate-lyase (PFL) is an important enzyme in the metabolic pathway of lactic acid bacteria (LAB) and is held responsible for the regulation of the shift between homolactic acid to mixed acid fermentation. PFL catalysis the reversible reaction of acetyl-CoA and formate into pyruvate and CoA. A glycyl radical, who is regenerated within the reaction, is involved; therefore, PFL works only under strictly anaerobic conditions. For its activation, the C-terminal domain has to bind to the
...

Person responsible: Stefan Henrich

Assays: Pyruvate formate-lyase (PFL): literature review, structure analysis and ...

No description specified

Person responsible: Falko Krause

Assays: No Assays

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