112 Studies visible to you, out of a total of 242

Key enzymes of critical points in the pathways will be targeted for disruption by the generation of RNAi cell lines and lines which drive tetracycline-regulatable ectopic over expression of either wild type enzyme or, if appropriate, dominant-negative or mis-targeted mutants of these. In all cases perturbed lines will be analysed with respect to the mRNA, protein or enzymatic activities of other components of the subsystem, this being directed iteratively by the predictions from systems modelling.

Assays: Generation of RNAi cell lines in T.brucei brucei 2T1 cell line, Induction of RNA interference in T.brucei brucei 2T1 cell line

We have already demonstrated that the key metabolites of polyamine biosynthesis (arginine, ornithine, putrescine and spermidine) can be identified using HILIC chromatography coupled to the Orbitrap mass spectrometer, as can glycine, glutamate and cysteine used in glutathione biosynthesis, glutathionyl spermidine and trypanothione itself. Furthermore the key metabolites of the methionine cycle (methionine, S-adenosyl methionine, decarboxylated S-adenosyl methionine, methylthioadenosine) can all

Assays: Intracellular metabolite concentrations in T. brucei under pH stress

This study aims to establish the optimum conditions and assay methods for measuring ATP levels

Person responsible: Martin Valachovic

Assays: Compare ATP extraction methods

For cells to accurately read out the genomic content, high fidelity during transcription is required. This is mainly established by the accuracy of the active centre of RNA polymerase (RNAP). Based on in vitro experiments with Escherichia coli RNAP it was also suggested that proofreading of transcription via RNA hydrolysis by RNAP may contribute to overall fidelity and processivity. RNAP’s intrinsic cleavage activity is stimulated by the highly conserved Gre factors suggesting that Gre factors

Assays: RNA-Seq

Genotype: Wildtype (M145E)
Medium: Phosphate-limited (F134)

Assays: Online/offline measurements, metabolomics, proteomics, transcriptomics

Multi experimental approach to define the gene expression remodelling under potassium starvation conditions.

Person responsible: Falko Krause

Assays: LacZ Reporters, RT-PCR, Time Course Micro Array Experiment

No description specified

Assays: No Assays

Annotation retrieval

Person responsible: Sebastian Curth

Assays: No Assays

Growth of B.subtilis in shakeflask at 57°C; 16°C, 37°C(Control) and 1,2M NaCl in SMM.

Assays: Tiling array analysis of continuous growth stress conditions in SMM

Studies on the effect of deletion of genes encoding 14-3-3 proteins on the transcriptional response of yeast cells to potassium starvation.

Assays: No Assays

Contains copy number per locus tag at different times of Growth between 0.25h and 96 hours.
M. pneumoniae was grown in Batch, cells attached to the bottom of the flask (single cell layer), non stirred, non aerated.

Person responsible: Niels Zondervan

Assays: No Assays

Experiments using shake flask cultures to measure dynamics associated with sigB response.

Assays: No Assays

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