Phosphoglycerate kinase acts as a futile cycle at high temperature

The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde 3-phosphate (although the latter was degraded at 70C) using phosphoglycerate kinase and glyceraldehyde 3-phosphate dehydrogenase.

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Created: 27th Sep 2017 at 14:36

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