Phosphoglycerate kinase acts as a futile cycle at high temperature

The gluconeogenic conversion of 3-phosphoglycerate via 1,3-bisphosphoglycerate to glyceraldehyde-3-phosphate was compared at 30 C and at 70 C. At 30 C it was possible to produce 1,3-bisphosphoglycerate from 3-phosphoglycerate with phosphoglycerate kinase, but at 70 C, 1,3- bisphosphoglycerate was dephosphorylated rapidly to 3-phosphoglycerate, effectively turning the phosphoglycerate kinase into a futile cycle. At both temperatures it was possible to convert 3-phosphoglycerate to glyceraldehyde 3-phosphate (although the latter was degraded at 70C) using phosphoglycerate kinase and glyceraldehyde 3-phosphate dehydrogenase.

help Contributor and Creators

Views: 437

Created: 27th Sep 2017 at 14:36

Related items

Powered by
Seek new full
Copyright © 2008 - 2018 The University of Manchester and HITS gGmbH