Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. By operating at two different flow rates per experiment, we observe four of categories of cell behaviour. The present model (gustavsson3) predicts the steady-state to limit cycle transition observed in 40.2% of the cell population.
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Created: 23rd Jul 2012 at 23:24
Last updated: 23rd Jul 2012 at 01:49
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