Kinetic characterisation of phosphoglycerate kinase
Contributor: Jacky Snoep
Assay type: Enzymatic Assay
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model Gluconeogenesis
Organisms: Sulfolobus solfataricus
SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts
Data files: PGK Kinetic Data
PGK - GAPDH models
Biological problem addressed: Model Analysis Type
Investigation: Phosphoglycerate kinase acts as a futile cycle ...
Study: PGK-GAPDH 30C & 70C
Organisms: No organisms
Models: PGK-GAPDH model Sulfolobus kouril8, PGK-GAPDH model yeast kouril7, PGK-GAPDH models yeast and Sulfolobus Fig. 4
SOPs: No SOPs
Data files: No Data files
Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.
Assay type: Metabolite Concentration
Technology type: Technology Type
Data files: PGK yeast with recycling of ATP, PGK yeast without recycling of ATP
A model for the PGK reaction of yeast in presence or absence of the ATP recycling reactions
Models: PGK yeast Fig1a, PGK yeast with/without recycling
Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically
Contributor: Theresa Kouril
Assay type: Experimental Assay Type
Technology type: NMR
Data files: Sso PGK (14 ug/ml) in presence of recycling sys..., Sso PGK (28 ug/ml) in presence of recycling sys..., Sso PGK in absence of recycling system, 31P NMR..., Sso PGK in presence of ATP recycling system at ...
PGK 70C model
Models: PGK 70C, PGK 70C SBML
Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.
Biological problem addressed: Enzymology
Models: PGK Kinetic Model
Data files: PGK Kinetic Data, PGK Kinetics Simulation
Data files: PGK-GAPDH 30, 3PG, GAP & BPG, PGK-GAPDH 30, PYR
Data files: PGK-GAPDH 70