Kinetic characterisation of phosphoglycerate kinase
Contributor: Jacky Snoep
Assay type: Enzymatic Assay
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model Gluconeogenesis
Organisms: Sulfolobus solfataricus
SOPs: Cloning and heterologous expression in E. coli, Preparation of cell extracts of recombinant E.coli, Protein purification from E. coli extracts
Data files: PGK Kinetic Data
Table describing the catabolite repression of β-xylosidase by different carbon sources(glucose, sorbitol, fructose, maltose, glycerol. mannitol) in various mutants of CcpA cofactors (HprK, crh)
Creator: Joerg Stuelke
Contributor: Leif Steil
Investigations: No Investigations
Studies: No Studies
Assays: No Assays
This method describes the preparation of the in vivo-like buffer for the measurement of bloodstream T. brucei recombinant enzymes under pseudo-physiological conditions.
Creators: Alejandro Leroux, Luise Krauth-Siegel
Contributor: Alejandro Leroux