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Metabolic changes of 26 intracellular metabolites extracted from T. b. brucei 427 wild type and arginine kinase knockout cells exposed to methylene blue
Creator: Dong-Hyun Kim
Submitter: Dong-Hyun Kim
Metabolic changes of 26 intracellular metabolites extracted from T. b. brucei 427 wild type and arginine kinase knockout cells under high pH stress (pH8.7)
Creator: Dong-Hyun Kim
Submitter: Dong-Hyun Kim
Intracellular concentrations of 50 metabolites measured by LC-MS using isotope ratio based mass spectrometry technique
Creator: Dong-Hyun Kim
Submitter: Dong-Hyun Kim
TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of GSH, and Spd.
Creator: Alejandro Leroux
Submitter: Alejandro Leroux
The file contains the initial rate measurements of TbTryS obtained under different substrate and product initial concentrations.
Creator: Alejandro Leroux
Submitter: Alejandro Leroux