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GAPDH Modelling

Mathematical model for GAPDH kinetics, saturation with BPG, NADPH, NADP, GAP and Pi

SEEK ID: https://fairdomhub.org/assays/227

Modelling Analysis

Jacky Snoep

Projects: SulfoSys

Investigation: Central Carbon Metabolism of Sulfolobus solfataricus

Study: Model Gluconeogenesis

Biological problem addressed: Enzymology

Organisms: Sulfolobus solfataricus

Model:
Data:
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Created: 1st Aug 2013 at 16:00

Last updated: 8th Nov 2017 at 15:21

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Mathematical model of a subset of reactions comprising the three most temperature sensitive intermediates of the gluconeogenic pathway in S. solfataricus

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GAPDH Kinetics Simulation
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Simulation results of GAPDH experimental data for BPG, NADPH, NADP, GAP, and Pi

Creators: Jacky Snoep, Theresa Kouril

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Relationship type: Simulation results

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GAPDH Kinetic Data
SulfoSys

Kinetic characterisation of GAPDH. Expermental data for enzyme reaction rates with increasing concentrations of BPG, NADPH, NADP, GAP and Pi.

Creators: Jacky Snoep, Theresa Kouril

Contributor: Jacky Snoep

Relationship type: Construction data

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GAPDH Kinetic Model
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Mathematical model for GAPDH kinetics, BPG, NADPH, NADP, GAP and Pi saturation.

Creator: Jacky Snoep

Contributor: Jacky Snoep

Download
Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus
SulfoSys

Abstract (Expand)

Four enzymes of the gluconeogenic pathway in Sulfolobus solfataricus were purified and kinetically characterized. The enzymes were reconstituted in vitro to quantify the contribution of temperature … instability of the pathway intermediates to carbon loss from the system. The reconstituted system, consisting of phosphoglycerate kinase, glyceraldehyde 3-phosphate dehydrogenase, triose phosphate isomerase and the fructose 1,6-bisphosphate aldolase/ phosphatase maintained a constant consumption rate of 3-phosphoglycerate and production of fructose 6-phosphate over a 1 hour period. Cofactors ATP and NADPH were regenerated via pyruvate kinase and glucose dehydrogenase. A mathematical model was constructed on the basis of the kinetics of the purified enzymes and the measured half-life times of the pathway intermediates. The model quantitatively predicted the systems uxes and metabolite concentrations. Relative enzyme concentrations were chosen such that half the carbon in the system was lost due to degradation of the thermolabile intermediates dihydroxyacetone phosphate, glyceraldehyde 3-phosphate and 1,3 bisphosphoglycerate, indicating that intermediate instability at high temperature can significantly affect pathway efficiency. This article is protected by copyright. All rights reserved.

Authors: Theresa Kouril, Dominik Esser, Julia Kort, Hans Westerhoff, Bettina Siebers, Jacky Snoep

Date Published: 20th Jul 2013

Journal: FEBS J.

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