Tools: qPCR, Northern analyses), Proteomics (2D-PAGE), mutant strain generation, Fluorecence based reporter gene analyses/single cell analyses, Protein chemical methods (protein overproduction, purification, enzymatic analyses, quantitative Western analyses), molecular biological techniques (RNA/DNA techniques
I am research assistant in the microbiology department at the Ludwig-Maximilians Universität in Munich (München), working at the chair of Prof. Kirsten Jung. In our SysMO consortium we generate biological data and work in close cooperation with the workgroup of Dr. Andreas Kremling of the Max-Planck-Institut für Dynamik komplexer technischer Systeme in Magdeburg who performs mathematical modeling. The topic of our workpackage deals with "K+ homeostasis in Escherichia coli", wherby the K+ transporters,
Tools: Deterministic models, including:- Dynamic modelling- Parameter estimation- Optimal experimental design- Dynamic optimization, Computational Systems Biology, Stochastic models, C programming, enzymatic analyses, data modeling, linux, ODE, Computational and theoretical biology
Roles: Vice Coordinator
Expertise: genome-scale modeling, enzyme kinetics, Metabolic Pathway Analysis and Engineering Microbial Physiology Modeling of Biological Networks Industrial Systems Biotechnology White Biotech..., dynamics and control of biological networks
Since August 2008 I am professor in Systems Biology at the VU University Amsterdam. My Systems Bioinformatics group focusses on systems biology with a special focus on integrative bioinformatics. It aims at forming bridges between the classical bottom-up approaches in systems biology and the more data-driven approaches in classical bioinformatics. We combine experimental, modeling and theoretical approaches to study cellular physiology, with an emphasis on metabolic networks.