High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis


In Bacillus subtilis and its relatives carbon catabolite control, a mechanism enabling to reach maximal efficiency of carbon and energy sources metabolism, is achieved by the global regulator CcpA (carbon catabolite protein A). CcpA in a complex with HPr-Ser-P (seryl-phosphorylated form of histidine-containing protein, HPr) binds to operator sites called catabolite responsive elements, cre. Depending on the cre box position relative to the promoter, the CcpA/HPr-Ser-P complex can either act as a positive or a negative regulator. The cre boxes are highly degenerate semi-palindromes with a lowly conserved consensus sequence. So far, studies aimed at revealing how CcpA can bind such diverse sites were focused on the analysis of single cre boxes. In this study, a genome-wide analysis of cre sites was performed in order to identify differences in cre sequence and position, which determine their binding affinity.

SEEK ID: https://fairdomhub.org/publications/190

PubMed ID: 22900538

Projects: BaCell-SysMO

Journal: BMC Genomics


Date Published: 17th Aug 2012

Authors: Bogumila Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar Kuipers

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Views: 2643

Created: 7th Jan 2013 at 12:37

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