Regulation of the activity of lactate dehydrogenases from four lactic acid bacteria

Despite high similarity in sequence and catalytic properties, the L-lactate dehydrogenases (LDH) in lactic acid bacteria (LAB) display differences in their regulation which may arise from their adaptation to different habitats. We combined experimental and computational approaches to investigate the effects of fructose-1,6-bisphosphate (FBP), phosphate (Pi) and ionic strength (NaCl concentration) on 6 LDHs from 4 LABs studied at pH 6 and pH 7. We find: (1) The extent of activation by FBP (Kact) differs: L. plantarum LDH is not regulated by FBP; the other LDHs are activated with increasing sensitivity in the following order: E. faecalis LDH2 ≤ L. lactis LDH2

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Created: 19th Feb 2014 at 14:08

Last updated: 8th Nov 2017 at 15:21

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