Selected Cell
Cell:
Value:
array design
experiment_sample
data sheet
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
Array design description | |||||||||
FORMAT | |||||||||
ARRAYBATCH | Simon Rasmussen, Henrik Bjørn Nielsen and Hanne Jarmer*. (2009) The transcriptionally active regions in the genome of Bacillus subtilis, Molecular Microbiology, 73, 1043–1057 | The array design specification consists of the description of the common features of the array as the whole, and the description of each array design elements (e.g., each spot). Following terminology used in MAGE, we distinguish between three levels of array design elements: feature - the location on the array, reporter - the nucleotide sequence present in a particular location on the array, and composite sequence - a set of reporters used collectively to measure an expression of a particular gene, exon, or splice-variant. The details that should be given of each of them are described below. | |||||||
ARRAYBATCHNAME | Tiling Array | * array design name | |||||||
ARRAYTYPE | Nimblegen | * platform type: in situ synthesized, spotted or other | |||||||
SPOTPARAMID | BaSysBio Bacillus subtilis T1385K array version 1’ | database ID | |||||||
NUMBERBLOCKSPERCHIP | * physical dimensions of array support (e.g. of slide) | ||||||||
NUMBERBLOCKSHORIZ | 43570 | * number of features on the array | |||||||
NUMBERBLOCKSVERTIC | |||||||||
NUMBERROWSINBLOCK | |||||||||
NUMBERCOLUMNSINBLOCK | date of production | ||||||||
SPOTTINGDATE | |||||||||
COMMENTS | something like surface and coating specification etc | ||||||||
PROVIDERID | Nimblegen | * availability (e.g., for commercial arrays) or production protocol for custom made arrays | |||||||
ARRAY | single array of certain array batch | ||||||||
ARRAYID | |||||||||
SOM03FS0 - SN: 1=37°C_Control - repl. 1 | |||||||||
SOM03FS1 - SN: 2=37°C_Control - repl. 2 | |||||||||
SOM03FS2 - SN: 3=37°C_Control - repl. 3 | |||||||||
SOM03G3T - SN: 19=57°C_- SMM - repl. 1 | |||||||||
SOM03G3V - SN: 20=57°C_- SMM - repl. 2 | |||||||||
SOM03G3W - SN: 21=57°C_- SMM - repl. 3 | |||||||||
ARRAYBATCHID | |||||||||
SUPPORTID | |||||||||
COMMENTARE | |||||||||
SLIDE_NB | |||||||||
REPORTER | oligo/sequence on array , related information, common for all arrays in one batch | ||||||||
RPROBEID | database ID | ||||||||
SPOTPARAMID | database ID, foreign key to arraybatch | ||||||||
TYPE | synthetic oligo-nucleotides | * the type of the reporter: synthetic oligo-nucleotides, PCR products, plasmids, colonies, other | |||||||
STRAND | single stranded | * single or double stranded | |||||||
SEQUENCE | |||||||||
LENGHT | Simon Rasmussen, Henrik Bjørn Nielsen and Hanne Jarmer*. (2009) The transcriptionally active regions in the genome of Bacillus subtilis, Molecular Microbiology, 73, 1043–1057 | * approximate lengths if exact sequence not known | |||||||
PROBETYPE | oligo, cDNA etc | ||||||||
ACC NUMBER | * gene name and links to appropriate databases (e.g., SWISS-PROT, or organism specific databases), if known and relevant | ||||||||
PROVIDER | |||||||||
PROVIDERID | o sequence accession number in DDBJ/EMBL/GenBank, if exists | ||||||||
ORGANISM | NCBI Accession number | ||||||||
CHROMOSOMENR | |||||||||
CHROM_BAND | |||||||||
SYMBOL | standartized name of gene | ||||||||
PLASMIDNR | |||||||||
SYMBOL | |||||||||
AMPLIFICATIONPROTOCOLID | database ID, foreign key to amplification protokol (separate) | ||||||||
PURIFICATIONPROTOCOLID | database ID, foreign key to purification protokol (separate) | ||||||||
PRODUCTIONDATE | |||||||||
DNAYIELD | amount of DNA on the spot | ||||||||
A
B
C
D
E
F
G
H
I
J
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
II Experiment description | |||||||||
INSTITUTE | Ernst-Moritz-Arndt-University of Greifswald | ||||||||
INSTITUTEID | http://www.medizin.uni-greifswald.de/funkgenom/institute.html | database ID | |||||||
INSTITUTENAME | Interfaculty Institute for Genetics and Functional Genomics | ||||||||
Project_NAME | BaCell-SysMo | ||||||||
LABNAME | Department of Functional Genomics | ||||||||
NEW- "CONTACT DETAILS" | email: praveen.kumar@uni-greifswald.de Friedrich-Ludwig-Jahn-Straße 15 a 17487 Greifswald, Germany. | ||||||||
whatever you need more | |||||||||
EXPERIMENT | This section that is common to all the hybridizations done in the experiment, such as the goal, brief description, experimental factors tested. It includes the following. | ||||||||
EXPERIMENTID | Continuous growth at 57°C and Control culture at 37°C | database ID | |||||||
EXPERIMENTNAME | |||||||||
EXP_TYPE | Treatment type | * normal vs. diseased comparison or * treated vs. untreated comparison *time course | |||||||
SUBMITTER | Praveen kumar sappa | ||||||||
EXPLANATION | B. subtilis was grown in SMM at 57°C and sample for RNA was harvested at OD1.0 at OD578 nm . Three biological replicates were analysed for Microarray. | Experimental factors, i.e. parameters or conditions tested, for instance, | |||||||
START_DATE | |||||||||
END_DATE | |||||||||
praveen.kumar@uni-greifswald.de | |||||||||
ORGANISM (for sample preparation) | |||||||||
ORGANISMID | database ID | ||||||||
NCBI ID | * organism (NCBI taxonomy) | ||||||||
ORGANISMNAME | Bacillus subtilis (168 trp+) | Ecoli | |||||||
STRAIN | 168 trp+ | o animal/plant strain or line | |||||||
DEVELOPMENTAL_STAGE | o development stage | ||||||||
INVIVOTREATMENT | * in vivo treatments (organism or individual treatments) | ||||||||
GENOTYPE | 168 trp+ | o genetic variation (e.g., gene knockout, transgenic variation) | |||||||
PHENOTYPE | non-sporulating | o individual genetic characteristics (e.g., disease alleles, polymorphisms) | |||||||
SAMPLETREATMENTPROTOCOL (NEW - "STORAGE AND CULTURING PROCEDURES" ) | After harvesting cells rapidly they were centrifuged at 4 °C for 3 min at 8000 rpm. The cell pellets were frozen in liquid nitrogen and stored at -80 °C. | ||||||||
GROW_MEDIUM | SMM | * growth conditions | |||||||
GROW_TEMPERATURE | 57°C | * growth conditions | |||||||
COMMENTS | |||||||||
By a sample we understand the biological material (biomaterial), from which the nucleic acids have been extracted for subsequent labeling and hybridization. In this section all steps that precedes the hybridization with the array are described. We can usually distinguish between the source of the sample (bio-source, e.g., organism, cell type or line), its treatment, the extract preparation, and its labeling. | |||||||||
CELLSOURCE | Biomaterial manipulations: laboratory protocol, including relevant parameters, e.g., | ||||||||
CELLSOURCEID | database ID | ||||||||
PATIENTID | database ID, foreign key to organism | ||||||||
NEW- "Growth stage of culture" | exponentially phase and starvation | ||||||||
NEW - "SAMPLE VOLUME_ML" | 15 OD units corresponding to harvesting time point. | ||||||||
VOLUME_ML | 100 | ||||||||
NUMB_OF_ALIQUOTS | 2 aliquots | ||||||||
SAMPLE_CONDITION | 37°C_Control - repl. 1 37°C_Control - repl. 2 37°C_Control - repl. 3 57°C_- SMM - repl. 1 57°C_- SMM - repl. 2 57°C_- SMM - repl. 3 | ||||||||
CELLSOURCEISOLDATE | |||||||||
EXPERIMENTATOR | Praveen kumar Sappa | ||||||||
COMMENT | |||||||||
AGE_AT_SAMPLING | |||||||||
DATEOFSAMPLEPREPARATION | |||||||||
NA_ISOLATION | DNA, RNA etc isolation info | preparation protocol for each extract prepared from the sample | |||||||
NAISOLATIONID | database ID | ||||||||
CELLSOURCEID | database ID | ||||||||
REFERENCE | |||||||||
TYPE | extraction of total RNA | * whether total RNA, mRNA, or genomic DNA is extracted | |||||||
NAISOLATIONDATE | 2009-02-19T0:0:0+0000 | ||||||||
NAISOLATIONPROTOCOLID | |||||||||
NAISOLATIONCOMMENT | RNA quality was checked with Nanodrop and Bioanalyzer | ||||||||
NAMODIFICATION | DNA SONICATION, DNA-PROTEIN CROSSLINKING, Antibodies used and Immuno-precipitation, DNA ISOLATION | ||||||||
YIELD_UG | |||||||||
CONCENTRATION_UG_PER_ML NEW | |||||||||
EXPERIMENTATOR | Praveen kumar Sappa | ||||||||
TREAT_DNASE | samples were treated with Rnase free Dnase I | ||||||||
CONC_TUB | |||||||||
CHECK_RESULT | Absence of DNA was checked with Bioanalyser | ||||||||
SAMPLE | Samples used, extract preparation and labeling | ||||||||
SAMPLEID | database ID | ||||||||
NAISOLATIONID | database ID | ||||||||
LABELTYPE | * label used (e.g., A-Cy3, G-Cy5, 33P, ..) | 5) External controls added to hybridization extract(s) (spiking controls) | |||||||
NEW - "Labeling of control DNA" | |||||||||
NEW - "Labeling of test DNA" | |||||||||
LABELINGDATE | Hybridization was done at Roche NimbleGen, Inc | ||||||||
LABELINGPROTOCOLID | |||||||||
LABELINGCOMMENT | * label incorporation method | ||||||||
LABELINCORPORATIONRATE | |||||||||
LABELREFERENCEDATE | |||||||||
SPIKEDNAQUANTITY_NG | * element on array expected to hybridize to spiking control | ||||||||
SPIKINGID | * spike type (e.g., oligonucleotide, plasmid DNA, transcript) | ||||||||
MIX_NAID | * spike qualifier (e.g., concentration, expected ratio, labelling methods if different than that of the extract) | ||||||||
HYBRIDISATION | |||||||||
NEW- "REPLICA OF EXPERIMENT" | EXPERIMENTID | ||||||||
sampleid (mehrere möglich) | Hybridization was done at Roche NimbleGen, Inc | Reference is the pool of all the samples. Each sample contributes equal amount of RNA to the pool. | |||||||
HYBRIDISATIONID | database ID | ||||||||
EXPERIMENTID | database ID | ||||||||
ARRAYID | database ID | ||||||||
HYBPROTID | |||||||||
DATE_OF_HYBRIDISATION | |||||||||
DESCRHYBINSTRUMENTS | |||||||||
HYBPROTOCOL | * quantity of labeled target used | ||||||||
- | * time, concentration, volume, temperature | ||||||||
- | * description of the hybridization instruments | ||||||||
- | * the solution (e.g., concentration of solutes), * wash procedure | ||||||||
- | |||||||||
COMMENTS | |||||||||
Measurement data | We distinguish between three levels of data processing - raw data (images), image quantitations and gene expression data matrix. Each hybridization has at least one image, each image has a corresponding image quantitation table, where a row represents an array design element and a column to a different quantitation types, such as mean or median pixel intensity. Several quantitation tables can be combined using data processing metrics to obtain the 'final' gene expression measurement table associated with the experiment. | ||||||||
* for each scan laboratory protocol for scanning, including scanning hardware and software, scan parameters, including laser power, spatial resolution, pixel space, PMT voltage; | |||||||||
* scanned images; | |||||||||
Image analysis and quantitation | |||||||||
NEW -"RESULTS OF AGILENT FEATURE EXTRACTION (tab delimited txt))" | |||||||||
NEW - "Normalisation method" | |||||||||
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
4601
4602
4603
4604
4605
4606
4607
4608
4609
4610
4611
4612
4613
4614
4615
4616
4617
4618
4619
4620
4621
4622
4623
4624
4625
4626
4627
4628
4629
4630
4631
4632
4633
4634
4635
4636
4637
4638
4639
4640
4641
4642
4643
4644
4645
4646
4647
4648
4649
4650
4651
4652
4653
4654
4655
4656
4657
4658
4659
4660
4661
4662
4663
4664
4665
4666
4667
4668
4669
4670
4671
4672
4673
4674
4675
4676
4677
4678
4679
4680
4681
4682
4683
4684
4685
4686
4687
4688
4689
4690
4691
4692
4693
4694
4695
4696
4697
4698
4699
4700
4701
4702
4703
4704
4705
4706
4707
4708
4709
4710
4711
4712
4713
4714
4715
4716
4717
4718
4719
4720
4721
4722
4723
4724
4725
4726
4727
4728
4729
4730
4731
4732
4733
4734
4735
4736
4737
4738
4739
4740
4741
4742
4743
4744
4745
4746
4747
4748
4749
4750
4751
4752
4753
4754
4755
4756
4757
4758
4759
4760
4761
4762
4763
4764
4765
4766
4767
4768
4769
4770
4771
4772
4773
4774
4775
4776
4777
4778
4779
4780
4781
4782
4783
4784
4785
4786
4787
4788
4789
4790
4791
4792
4793
4794
4795
4796
4797
4798
4799
4800
yoaK | BSU18640 | 8.4547 | 8.5665 | 8.7281 | 8.5276 | 8.9388 | 8.8735 | ||||||||||||
yoaM | BSU18660 | 7.0273 | 7.1702 | 7.2102 | 7.2975 | 7.0779 | 7.2227 | ||||||||||||
yoaN | BSU18670 | 9.7779 | 9.5061 | 9.636 | 7.328 | 6.9989 | 7.2891 | ||||||||||||
yoaO | BSU18680 | 10.2588 | 10.1567 | 10.4487 | 9.8544 | 9.5863 | 10.2752 | ||||||||||||
yoaP | BSU18690 | 9.0756 | 9.2573 | 9.298 | 9.1099 | 8.8681 | 9.5028 | ||||||||||||
yoaQ | BSU18700 | 8.6422 | 8.5397 | 8.2228 | 7.886 | 7.8726 | 8.0791 | ||||||||||||
yoaR | BSU18720 | 8.1479 | 7.9686 | 7.7422 | 7.4218 | 6.9941 | 7.2095 | ||||||||||||
yoaS | BSU18730 | 9.184 | 9.4197 | 9.8778 | 9.557 | 9.5 | 9.4215 | ||||||||||||
yoaT | BSU18750 | 8.6333 | 8.817 | 9.0071 | 8.9981 | 9.1972 | 8.9905 | ||||||||||||
yoaU | BSU18760 | 7.9177 | 8.0048 | 8.0218 | 8.1361 | 8.0873 | 8.2751 | ||||||||||||
yoaW | BSU18780 | 10.477 | 10.7016 | 10.4138 | 9.0952 | 9.4754 | 9.9308 | ||||||||||||
yoaZ | BSU18790 | 9.3698 | 9.6278 | 9.6091 | 9.5826 | 9.0834 | 9.5216 | ||||||||||||
yobA | BSU18810 | 9.3595 | 9.4952 | 9.3772 | 9.0974 | 8.779 | 9.0169 | ||||||||||||
yobB | BSU18820 | 10.3366 | 10.8383 | 10.3337 | 9.1855 | 8.7317 | 8.8835 | ||||||||||||
yobD | BSU18850 | 11.9691 | 11.8482 | 11.9746 | 10.2699 | 12.5874 | 12.7377 | ||||||||||||
yobF | BSU18890 | 11.8012 | 11.5819 | 11.7828 | 11.1012 | 12.1944 | 12.4015 | ||||||||||||
yobI | BSU18970 | 12.6726 | 12.7486 | 13.1834 | 12.2982 | 12.9383 | 12.9595 | ||||||||||||
yobJ | BSU18980 | 14.5549 | 14.8584 | 15.2346 | 12.9714 | 13.2338 | 13.1615 | ||||||||||||
yobK | BSU18990 | 12.3813 | 12.7075 | 12.8178 | 11.8745 | 12.1209 | 12.4308 | ||||||||||||
yobL | BSU19000 | 11.3838 | 11.3961 | 11.5436 | 10.5399 | 10.8818 | 11.1547 | ||||||||||||
yobN | BSU19020 | 7.3307 | 7.5097 | 7.5622 | 7.2768 | 7.0468 | 7.1969 | ||||||||||||
yobO | BSU19030 | 9.1625 | 9.5944 | 9.7096 | 9.6558 | 8.4557 | 8.5727 | ||||||||||||
yobQ | BSU19050 | 11.3614 | 11.1791 | 11.3359 | 10.6627 | 11.8898 | 12.0706 | ||||||||||||
yobR | BSU19060 | 11.7298 | 11.5437 | 11.6088 | 10.76 | 12.4256 | 12.4646 | ||||||||||||
yobS | BSU19070 | 8.8705 | 9.095 | 9.1026 | 10.1805 | 10.671 | 10.6425 | ||||||||||||
yobT | BSU19080 | 9.2178 | 9.2925 | 9.2204 | 10.7965 | 10.9801 | 11.0374 | ||||||||||||
yobU | BSU19090 | 7.3192 | 7.567 | 7.8109 | 7.6119 | 7.4524 | 7.6679 | ||||||||||||
yobV | BSU19100 | 9.026 | 8.9968 | 9.1049 | 7.9533 | 8.4881 | 8.5737 | ||||||||||||
yobW | BSU19110 | 9.1867 | 8.9506 | 8.8295 | 7.167 | 6.9905 | 7.2092 | ||||||||||||
yocA | BSU19130 | 13.4891 | 13.7486 | 13.658 | 11.6319 | 12.8396 | 12.8844 | ||||||||||||
yocB | BSU19150 | 8.2894 | 8.3128 | 8.4733 | 12.105 | 9.1953 | 9.7152 | ||||||||||||
yocC | BSU19160 | 10.0581 | 10.0624 | 9.9768 | 9.4295 | 10.3767 | 10.3586 | ||||||||||||
yocD | BSU19170 | 12.6157 | 12.5967 | 12.7326 | 10.8271 | 11.7681 | 12.2289 | ||||||||||||
yocH | BSU19210 | 13.6006 | 13.8623 | 14.0574 | 14.2183 | 13.3282 | 14.5702 | ||||||||||||
yocI | BSU19220 | 10.3554 | 10.4228 | 10.6202 | 10.3003 | 10.478 | 10.622 | ||||||||||||
yocJ | BSU19230 | 11.2942 | 11.2068 | 11.5614 | 9.9731 | 10.6812 | 10.6866 | ||||||||||||
yocK | BSU19240 | 10.2396 | 10.1517 | 10.2027 | 14.5948 | 9.8896 | 9.8876 | ||||||||||||
yocL | BSU19250 | 10.1862 | 10.003 | 10.1608 | 8.2616 | 8.7275 | 9.1508 | ||||||||||||
yocM | BSU19260 | 10.2538 | 10.2485 | 10.4219 | 8.0299 | 8.5814 | 9.0407 | ||||||||||||
yocN | BSU19280 | 11.9948 | 11.597 | 11.3508 | 8.725 | 8.6722 | 9.0954 | ||||||||||||
yocR | BSU19340 | 10.4231 | 10.461 | 10.6753 | 9.8336 | 10.2096 | 11.1339 | ||||||||||||
yocS | BSU19350 | 11.5171 | 11.3675 | 11.4912 | 12.0957 | 11.9359 | 13.0379 | ||||||||||||
yodA | BSU19530 | 8.1097 | 8.2651 | 8.2935 | 8.1506 | 8.184 | 8.2237 | ||||||||||||
yodB | BSU19540 | 10.0771 | 10.0393 | 10.0249 | 9.7789 | 10.1399 | 10.5049 | ||||||||||||
yodC | BSU19550 | 11.608 | 11.9617 | 12.2826 | 11.778 | 11.7202 | 12.0824 | ||||||||||||
yodD | BSU19560 | 8.8188 | 8.9071 | 8.9996 | 9.3084 | 8.9779 | 8.9985 | ||||||||||||
yodE | BSU19570 | 9.7735 | 9.8922 | 9.9826 | 9.5603 | 9.6985 | 9.6864 | ||||||||||||
yodF | BSU19580 | 12.4096 | 12.2143 | 12.3644 | 12.0781 | 11.5226 | 12.2829 | ||||||||||||
yodH | BSU19600 | 9.1228 | 9.2212 | 9.8714 | 7.4135 | 7.3522 | 7.525 | ||||||||||||
yodI | BSU19610 | 10.0683 | 9.9573 | 10.5614 | 8.115 | 7.636 | 7.9842 | ||||||||||||
yodJ | BSU19620 | 10.9825 | 11.1393 | 11.2301 | 11.0684 | 11.0475 | 10.7146 | ||||||||||||
yodL | BSU19640 | 10.6578 | 10.622 | 10.5249 | 10.0675 | 9.5795 | 10.1891 | ||||||||||||
yodM | BSU19650 | 8.8742 | 9.077 | 9.1146 | 8.6838 | 9.0934 | 8.9505 | ||||||||||||
yodN | BSU19670 | 8.8659 | 8.7745 | 9.5307 | 7.625 | 7.6863 | 7.9475 | ||||||||||||
yodP | BSU19700 | 9.321 | 8.9079 | 8.6415 | 7.4757 | 7.2066 | 7.3728 | ||||||||||||
yodQ | BSU19710 | 9.1303 | 8.9602 | 8.5898 | 7.5079 | 7.1966 | 7.3767 | ||||||||||||
yodR | BSU19720 | 9.5131 | 9.0672 | 8.7636 | 7.4412 | 7.249 | 7.3864 | ||||||||||||
yodS | BSU19730 | 9.3048 | 9.0496 | 8.5272 | 7.3156 | 7.1187 | 7.2222 | ||||||||||||
yodT | BSU19740 | 9.7315 | 9.3005 | 8.9267 | 7.2803 | 7.0937 | 7.1894 | ||||||||||||
yoeA | BSU18370 | 11.8558 | 11.8813 | 11.7753 | 10.7312 | 10.0246 | 10.5386 | ||||||||||||
yoeC | BSU18390 | 10.1662 | 10.1769 | 10.3466 | 10.2508 | 11.4804 | 11.6384 | ||||||||||||
yoeD | BSU18400 | 10.7642 | 10.7924 | 10.8013 | 9.0388 | 10.3661 | 10.836 | ||||||||||||
yogA | BSU18430 | 8.3226 | 8.53 | 8.8509 | 8.9252 | 8.7559 | 8.7709 | ||||||||||||
yojA | BSU19520 | 6.7074 | 7.0512 | 7.2074 | 7.5488 | 7.1279 | 7.2294 | ||||||||||||
yojB | BSU19510 | 10.2675 | 10.3004 | 10.9799 | 7.6026 | 7.461 | 7.4508 | ||||||||||||
yojE | BSU19480 | 9.6707 | 9.6852 | 9.7108 | 10.2512 | 10.7501 | 10.6593 | ||||||||||||
yojF | BSU19470 | 12.5737 | 12.8188 | 12.8346 | 12.1791 | 12.5072 | 12.6584 | ||||||||||||
yojG | BSU19460 | 12.274 | 12.4542 | 12.5735 | 12.1907 | 12.2026 | 12.4647 | ||||||||||||
yojJ | BSU19430 | 7.7787 | 7.5905 | 7.6129 | 9.1208 | 7.4232 | 7.3779 | ||||||||||||
yojK | BSU19420 | 10.2501 | 10.6081 | 10.7695 | 10.6873 | 10.4268 | 10.6366 | ||||||||||||
yojN | BSU19390 | 11.6968 | 11.791 | 11.8999 | 11.5568 | 12.4947 | 12.0243 | ||||||||||||
yojO | BSU19380 | 11.2394 | 11.2503 | 11.3648 | 11.7776 | 12.0465 | 11.6362 | ||||||||||||
yojW | BSU19999 | 8.3363 | 8.6102 | 9.1188 | 8.8218 | 9.0973 | 8.8669 | ||||||||||||
yokA | BSU21660 | 10.9132 | 11.0239 | 11.1608 | 11.461 | 12.0919 | 12.2038 | ||||||||||||
yokB | BSU21650 | 10.9632 | 10.9784 | 11.0246 | 10.3903 | 10.5528 | 10.7421 | ||||||||||||
yokC | BSU21640 | 10.1872 | 9.8828 | 10.2291 | 8.9909 | 9.5982 | 10.2113 | ||||||||||||
yokD | BSU21630 | 11.6288 | 11.9761 | 12.4876 | 11.0423 | 10.76 | 10.9221 | ||||||||||||
yokE | BSU21620 | 12.5269 | 12.7953 | 13.1372 | 11.6378 | 12.3249 | 12.1754 | ||||||||||||
yokF | BSU21610 | 11.7974 | 11.769 | 12.0681 | 10.5772 | 11.2968 | 11.1238 | ||||||||||||
yokG | BSU21600 | 13.6945 | 14.0425 | 14.2935 | 12.8315 | 12.6674 | 12.9486 | ||||||||||||
yokI | BSU21580 | 12.0172 | 11.9772 | 12.2841 | 10.892 | 11.2648 | 11.5532 | ||||||||||||
yokJ | BSU21570 | 12.5817 | 12.8192 | 13.0543 | 11.7123 | 12.1619 | 12.3784 | ||||||||||||
yokK | BSU21560 | 12.4437 | 12.7187 | 12.9984 | 12.3403 | 12.3895 | 12.4674 | ||||||||||||
yokL | BSU21550 | 12.8086 | 12.9507 | 13.2038 | 13.1073 | 12.8101 | 12.957 | ||||||||||||
yokU | BSU19689 | 9.4605 | 9.1544 | 9.0248 | 7.8357 | 7.5553 | 7.6559 | ||||||||||||
yolA | BSU21540 | 15.0405 | 15.163 | 15.7302 | 13.3003 | 14.2249 | 14.3122 | ||||||||||||
yolB | BSU21530 | 15.0437 | 15.1883 | 15.7525 | 13.4857 | 14.5187 | 14.5882 | ||||||||||||
yolC | BSU21520 | 11.1301 | 11.3005 | 11.244 | 10.126 | 9.7665 | 10.3429 | ||||||||||||
yolJ | BSU21450 | 14.4225 | 14.6853 | 15.2096 | 15.6165 | 15.6701 | 15.8731 | ||||||||||||
yomD | BSU21400 | 6.6132 | 7.0567 | 7.1888 | 7.3616 | 7.0729 | 7.4354 | ||||||||||||
yomE | BSU21390 | 6.6711 | 7.0463 | 7.2051 | 7.2499 | 6.8896 | 7.1495 | ||||||||||||
yomF | BSU21380 | 6.6317 | 7.0971 | 7.2844 | 7.4195 | 7.0502 | 7.1755 | ||||||||||||
yomG | BSU21370 | 7.8689 | 7.941 | 8.1868 | 7.6286 | 8.0833 | 8.3994 | ||||||||||||
yomH | BSU21360 | 6.9543 | 7.2584 | 7.371 | 7.3523 | 7.1048 | 7.5254 | ||||||||||||
yomI | BSU21350 | 7.4912 | 7.7435 | 7.9774 | 7.6051 | 7.4958 | 7.7604 | ||||||||||||
yomJ | BSU21340 | 12.6406 | 12.5069 | 12.8179 | 10.6785 | 11.5107 | 11.5367 | ||||||||||||
yomK | BSU21330 | 12.2611 | 12.2873 | 12.7354 | 9.3509 | 10.2789 | 10.4513 | ||||||||||||
yomM | BSU21300 | 7.1356 | 7.327 | 7.5726 | 7.9976 | 7.1868 | 7.3995 | ||||||||||||
yomN | BSU21290 | 7.2914 | 7.5913 | 7.7545 | 7.5609 | 7.6758 | 7.7411 | ||||||||||||
yomO | BSU21280 | 7.4513 | 7.7939 | 7.9908 | 7.5996 | 7.7692 | 7.7881 | ||||||||||||
yomP | BSU21270 | 7.3332 | 7.5786 | 7.8244 | 7.5084 | 7.3739 | 7.6144 | ||||||||||||
yomQ | BSU21260 | 7.3102 | 7.571 | 7.7239 | 7.3262 | 7.137 | 7.3843 | ||||||||||||
yomR | BSU21250 | 6.919 | 7.2122 | 7.4483 | 7.4102 | 7.0484 | 7.2271 | ||||||||||||
yomS | BSU21240 | 6.9562 | 7.2794 | 7.4779 | 7.5479 | 7.0256 | 7.2196 | ||||||||||||
yomT | BSU21230 | 7.1505 | 7.2809 | 7.5759 | 7.5551 | 7.0289 | 7.2241 | ||||||||||||
yomU | BSU21220 | 7.437 | 7.6441 | 7.8799 | 7.315 | 7.0801 | 7.2961 | ||||||||||||
yomV | BSU21210 | 7.5661 | 7.6713 | 7.9774 | 7.4615 | 7.1784 | 7.5387 | ||||||||||||
yomW | BSU21200 | 7.644 | 7.7003 | 7.9723 | 7.4257 | 7.1573 | 7.365 | ||||||||||||
yomX | BSU21190 | 7.3815 | 7.5348 | 7.8473 | 7.4225 | 7.1791 | 7.4033 | ||||||||||||
yomY | BSU21180 | 7.551 | 7.5888 | 7.893 | 7.3909 | 7.2035 | 7.457 | ||||||||||||
yomZ | BSU21170 | 7.5195 | 7.7391 | 7.879 | 7.4289 | 7.2221 | 7.4831 | ||||||||||||
yonA | BSU21160 | 7.7929 | 7.9173 | 8.1977 | 7.9542 | 7.4295 | 7.793 | ||||||||||||
yonB | BSU21150 | 7.84 | 8.0232 | 8.2692 | 7.8337 | 7.4103 | 7.7791 | ||||||||||||
yonC | BSU21140 | 7.9828 | 8.1706 | 8.5776 | 8.087 | 7.8915 | 8.1066 | ||||||||||||
yonD | BSU21130 | 8.0482 | 8.1305 | 8.4731 | 7.9257 | 7.8316 | 8.2066 | ||||||||||||
yonE | BSU21120 | 7.362 | 7.5959 | 7.7637 | 7.5454 | 7.9214 | 8.2647 | ||||||||||||
yonF | BSU21110 | 6.78 | 7.1205 | 7.2722 | 7.4723 | 7.0609 | 7.3386 | ||||||||||||
yonG | BSU21100 | 6.7974 | 7.1289 | 7.2685 | 7.3081 | 7.1691 | 7.3938 | ||||||||||||
yonH | BSU21090 | 7.4391 | 7.5405 | 7.7958 | 7.4801 | 7.3747 | 7.6098 | ||||||||||||
yonI | BSU21080 | 9.9565 | 9.964 | 10.4563 | 11.4196 | 11.3194 | 11.208 | ||||||||||||
yonJ | BSU21070 | 7.2467 | 7.42 | 7.5811 | 7.4664 | 7.2412 | 7.5419 | ||||||||||||
yonK | BSU21060 | 7.1315 | 7.4917 | 7.6452 | 7.3968 | 7.4382 | 7.8413 | ||||||||||||
yonN | BSU21050 | 7.5533 | 7.7192 | 7.9444 | 7.8062 | 7.2717 | 7.4509 | ||||||||||||
yonO | BSU21040 | 6.8053 | 7.1828 | 7.3739 | 7.4198 | 7.0393 | 7.2558 | ||||||||||||
yonP | BSU21030 | 7.2828 | 7.4753 | 7.6212 | 7.4852 | 7.5504 | 7.9578 | ||||||||||||
yonR | BSU21020 | 12.8369 | 12.4295 | 12.9044 | 9.8625 | 12.0053 | 12.1403 | ||||||||||||
yonS | BSU21010 | 13.0959 | 12.9627 | 13.4082 | 10.6465 | 12.1848 | 12.2694 | ||||||||||||
yonT | BSU21000 | 9.223 | 9.6758 | 10.2668 | 10.8032 | 9.3665 | 9.4363 | ||||||||||||
yonU | BSU20990 | 12.3882 | 12.3296 | 12.9644 | 12.4912 | 11.6358 | 12.1671 | ||||||||||||
yonV | BSU20980 | 7.1102 | 7.4786 | 7.734 | 7.5524 | 7.3218 | 7.5084 | ||||||||||||
yonX | BSU20970 | 8.7135 | 9.0845 | 9.7023 | 10.0438 | 8.8714 | 8.5786 | ||||||||||||
yopA | BSU20960 | 11.272 | 11.3925 | 11.6202 | 10.9935 | 10.4733 | 10.6358 | ||||||||||||
yopB | BSU20950 | 8.7844 | 8.842 | 9.0187 | 7.6257 | 8.0659 | 8.4783 | ||||||||||||
yopC | BSU20940 | 12.5099 | 12.6279 | 12.9485 | 11.7322 | 12.387 | 12.4747 | ||||||||||||
yopD | BSU20930 | 11.3682 | 11.2835 | 11.9297 | 10.7584 | 11.2333 | 11.2921 | ||||||||||||
yopE | BSU20920 | 11.7747 | 11.4423 | 11.7349 | 8.8601 | 11.1816 | 11.5171 | ||||||||||||
yopF | BSU20910 | 11.744 | 11.695 | 11.7451 | 8.9179 | 11.1093 | 11.3939 | ||||||||||||
yopG | BSU20900 | 11.629 | 11.3659 | 11.6189 | 8.9571 | 10.9834 | 11.1782 | ||||||||||||
yopH | BSU20890 | 12.0054 | 12.2691 | 12.5383 | 10.6206 | 11.5434 | 11.7966 | ||||||||||||
yopI | BSU20880 | 12.2792 | 12.4401 | 12.8153 | 11.441 | 11.9428 | 12.007 | ||||||||||||
yopJ | BSU20870 | 11.0593 | 11.2347 | 11.543 | 10.7011 | 11.066 | 10.8856 | ||||||||||||
yopK | BSU20860 | 10.3718 | 10.5201 | 11.2553 | 11.2563 | 10.2433 | 10.245 | ||||||||||||
yopL | BSU20850 | 14.0732 | 14.4827 | 14.7755 | 14.0616 | 13.769 | 13.885 | ||||||||||||
yopM | BSU20840 | 12.6292 | 12.5561 | 13.0422 | 11.6768 | 12.0925 | 11.7721 | ||||||||||||
yopN | BSU20830 | 11.7172 | 11.5486 | 12.1058 | 10.8478 | 11.1171 | 11.1987 | ||||||||||||
yopO | BSU20820 | 12.0007 | 11.6942 | 12.0543 | 10.3232 | 10.9172 | 11.1012 | ||||||||||||
yopP | BSU20810 | 12.08 | 11.9095 | 12.2784 | 11.4571 | 11.2324 | 11.4021 | ||||||||||||
yopQ | BSU20800 | 12.5872 | 12.5754 | 12.8228 | 12.4911 | 12.3675 | 12.4395 | ||||||||||||
yopR | BSU20790 | 12.5637 | 12.6822 | 13.0905 | 12.8629 | 12.4774 | 12.4206 | ||||||||||||
yopS | BSU20780 | 9.3668 | 9.3403 | 9.4944 | 8.6745 | 9.2645 | 9.9366 | ||||||||||||
yopT | BSU20770 | 11.3523 | 11.3654 | 11.8287 | 10.5958 | 11.3904 | 11.3312 | ||||||||||||
yopU | BSU20760 | 10.993 | 10.7679 | 11.3483 | 10.3442 | 10.622 | 10.7797 | ||||||||||||
yopV | BSU20750 | 8.7994 | 9.1013 | 9.3764 | 9.4652 | 9.0757 | 8.9105 | ||||||||||||
yopW | BSU20740 | 7.6848 | 7.9727 | 8.1858 | 8.207 | 7.9852 | 7.9405 | ||||||||||||
yopX | BSU20730 | 7.547 | 7.8349 | 8.0494 | 8.0981 | 7.8394 | 7.7705 | ||||||||||||
yopY | BSU20720 | 7.9689 | 8.212 | 8.3439 | 8.6541 | 8.1947 | 8.2065 | ||||||||||||
yopZ | BSU20710 | 7.8876 | 8.0327 | 8.2409 | 8.3517 | 8.1437 | 8.1633 | ||||||||||||
yoqA | BSU20700 | 7.7808 | 8.0772 | 8.2297 | 8.3315 | 8.0864 | 8.092 | ||||||||||||
yoqB | BSU20690 | 7.8016 | 8.0975 | 8.3479 | 8.2355 | 8.1401 | 8.3158 | ||||||||||||
yoqC | BSU20680 | 7.9752 | 8.2063 | 8.4385 | 8.3762 | 8.3131 | 8.3899 | ||||||||||||
yoqD | BSU20670 | 7.9748 | 8.1564 | 8.3865 | 8.2082 | 8.1338 | 8.3135 | ||||||||||||
yoqE | BSU20660 | 8.2071 | 8.6051 | 8.8532 | 8.924 | 8.6966 | 8.7579 | ||||||||||||
yoqF | BSU20650 | 8.2078 | 8.449 | 8.8268 | 8.7763 | 8.6125 | 8.6242 | ||||||||||||
yoqG | BSU20640 | 8.2273 | 8.3317 | 8.6204 | 8.6999 | 8.448 | 8.4391 | ||||||||||||
yoqH | BSU20630 | 8.1613 | 8.1817 | 8.47 | 9.1795 | 8.7057 | 8.6632 | ||||||||||||
yoqI | BSU20620 | 8.4525 | 8.3532 | 8.6451 | 9.2873 | 9.2325 | 9.024 | ||||||||||||
yoqJ | BSU20610 | 7.9725 | 8.1654 | 8.3629 | 8.7139 | 8.8111 | 8.7815 | ||||||||||||
yoqK | BSU20600 | 8.1675 | 8.2432 | 8.4771 | 9.4519 | 9.1034 | 9.2247 | ||||||||||||
yoqL | BSU20590 | 8.1848 | 8.2838 | 8.4729 | 8.982 | 8.9458 | 9.3135 | ||||||||||||
yoqM | BSU20580 | 10.1355 | 11.7289 | 11.1472 | 9.8346 | 9.0764 | 9.466 | ||||||||||||
yoqN | BSU20570 | 7.211 | 7.4762 | 7.7542 | 7.7919 | 7.6653 | 7.9623 | ||||||||||||
yoqO | BSU20560 | 11.9039 | 11.7132 | 12.2176 | 9.9608 | 10.9545 | 11.7515 | ||||||||||||
yoqP | BSU20550 | 7.4884 | 7.8099 | 7.9459 | 7.7281 | 7.9398 | 8.144 | ||||||||||||
yoqR | BSU20540 | 7.581 | 7.8716 | 8.1971 | 7.9046 | 8.0208 | 8.305 | ||||||||||||
yoqS | BSU20530 | 7.7896 | 7.97 | 8.1822 | 8.1032 | 8.1617 | 8.4695 | ||||||||||||
yoqT | BSU20520 | 7.1451 | 7.4225 | 7.4838 | 7.4533 | 7.3693 | 7.5592 | ||||||||||||
yoqU | BSU20510 | 7.2775 | 7.5225 | 7.632 | 7.6181 | 7.641 | 7.8604 | ||||||||||||
yoqX | BSU20480 | 7.0177 | 7.4274 | 7.4517 | 7.6247 | 7.1776 | 7.5392 | ||||||||||||
yoqY | BSU20470 | 6.8 | 7.1505 | 7.2948 | 7.5173 | 7.0693 | 7.3124 | ||||||||||||
yoqZ | BSU20460 | 7.6795 | 7.8758 | 8.0366 | 7.6003 | 7.5108 | 7.8115 | ||||||||||||
yorA | BSU20450 | 8.0381 | 8.0963 | 8.2466 | 10.058 | 8.0139 | 8.1747 | ||||||||||||
yorB | BSU20440 | 11.2608 | 11.2374 | 11.6182 | 11.2974 | 10.8942 | 11.1218 | ||||||||||||
yorC | BSU20430 | 10.133 | 10.2345 | 10.2975 | 9.4117 | 9.8106 | 10.145 | ||||||||||||
yorD | BSU20420 | 15.4658 | 15.4881 | 16.1245 | 12.5528 | 13.5675 | 13.7015 | ||||||||||||
yorE | BSU20410 | 8.616 | 8.8385 | 9.3973 | 8.8786 | 7.8911 | 8.011 | ||||||||||||
yorF | BSU20400 | 8.2462 | 8.4285 | 8.9192 | 8.8465 | 7.9518 | 7.9286 | ||||||||||||
yorG | BSU20390 | 7.7083 | 8.0116 | 8.3688 | 7.8839 | 7.5586 | 7.5824 | ||||||||||||
yorH | BSU20380 | 7.3235 | 7.571 | 7.9202 | 7.5385 | 7.3436 | 7.515 | ||||||||||||
yorI | BSU20370 | 7.5848 | 7.8253 | 8.0409 | 7.9146 | 7.7761 | 7.802 | ||||||||||||
yorJ | BSU20360 | 7.2926 | 7.5304 | 7.7941 | 7.8864 | 7.5697 | 7.7094 | ||||||||||||
yorK | BSU20350 | 7.1477 | 7.3984 | 7.6017 | 8.0397 | 7.6618 | 7.66 | ||||||||||||
yorL | BSU20340 | 7.5998 | 7.8001 | 8.0378 | 8.0046 | 8.1222 | 8.278 | ||||||||||||
yorM | BSU20330 | 7.8728 | 8.0606 | 8.408 | 8.1588 | 7.78 | 7.9012 | ||||||||||||
yorN | BSU20320 | 9.7303 | 9.6497 | 10.1354 | 8.3268 | 9.2722 | 9.801 | ||||||||||||
yorO | BSU20310 | 9.4484 | 9.4933 | 9.9581 | 8.4085 | 9.1397 | 9.4981 | ||||||||||||
yorP | BSU20300 | 8.9774 | 9.0491 | 9.6198 | 10.0472 | 8.8015 | 9.0157 | ||||||||||||
yorQ | BSU20290 | 9.1394 | 9.1339 | 9.6263 | 9.9464 | 8.8116 | 9.186 | ||||||||||||
yorR | BSU20280 | 8.8467 | 8.9285 | 9.3536 | 9.5474 | 8.625 | 8.8805 | ||||||||||||
yorS | BSU20270 | 8.6289 | 8.7687 | 9.1084 | 9.0252 | 8.4564 | 8.7251 | ||||||||||||
yorT | BSU20260 | 8.4551 | 8.4268 | 8.6143 | 8.6245 | 8.0958 | 8.2346 |