Selected Cell
Cell:
Value:
final
Tabelle3
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
AA
AB
AC
AD
AE
AF
AG
AH
AI
AJ
AK
AL
AM
AN
AO
AP
AQ
AR
AS
AT
AU
AV
AW
AX
AY
AZ
BA
BB
BC
BD
BE
BF
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
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225
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230
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232
233
234
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236
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333
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198 | 11747307 | Saccharomyces cerevisiae | mutant A458K | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | Vmax [U/mg] | 432 | 8 | 444 | 8 | 6.2 | 25 | 110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP | FBP>=no | Vmax | |||||||||||||||||||||||||||||||||||||||||||
199 | 11747307 | Saccharomyces cerevisiae | mutant A458K | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | S0,5 | 2.5 | 0.1 | 0.22 | 0.01 | 6.2 | 25 | 110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP | no>FBP | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
200 | 11747307 | Saccharomyces cerevisiae | mutant A458K | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | nHill | 2.7 | 0.2 | 1 | 0.1 | 6.2 | 25 | 110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP | no>FBP | nHill | |||||||||||||||||||||||||||||||||||||||||||
201 | 11747307 | Saccharomyces cerevisiae | mutant R459Q | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | Vmax [U/mg] | 295 | 6 | 300 | 6 | 6.2 | 25 | 110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP | FBP>=no | Vmax | |||||||||||||||||||||||||||||||||||||||||||
202 | 11747307 | Saccharomyces cerevisiae | mutant R459Q | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | S0,5 | 2 | 0.1 | 2 | 0.01 | 6.2 | 25 | 110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP | no>=FBP | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
203 | 11747307 | Saccharomyces cerevisiae | mutant R459Q | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | nHill | 2.2 | 0.2 | 2.2 | 0.2 | 6.2 | 25 | 110 mM Mes, 335 mM KCl, 28 mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 µM β-NADH, 8.2 mM ADP, + - 2.4 mM FBP | no=FBP | nHill | |||||||||||||||||||||||||||||||||||||||||||
204 | 11747307 | Saccharomyces cerevisiae | WT | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | Vmax [U/mg] | 113 | 3 | 122 | 2 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | FBP>no | Vmax | |||||||||||||||||||||||||||||||||||||||||||
205 | 11747307 | Saccharomyces cerevisiae | WT | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | S0,5 | 1.4 | 0.1 | 0.12 | 0.01 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | no>FBP | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
206 | 11747307 | Saccharomyces cerevisiae | WT | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | nHill | 1.8 | 0.1 | 1 | 0.1 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | no>FBP | nHill | |||||||||||||||||||||||||||||||||||||||||||
207 | 11747307 | Saccharomyces cerevisiae | mutant Q299N | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | S0,5 | 4.9 | 0.1 | 0.13 | 0.01 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | unstable mutants | no>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
208 | 11747307 | Saccharomyces cerevisiae | mutant Q299N | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | nHill | 2.5 | 0.1 | 0.97 | 0.08 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | unstable mutants | no>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||
209 | 11747307 | Saccharomyces cerevisiae | mutant R369A | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | Vmax [U/mg] | 39 | 0.6 | 38 | 3 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | unstable mutants | FBP>=no | Vmax | ||||||||||||||||||||||||||||||||||||||||||
210 | 11747307 | Saccharomyces cerevisiae | mutant R369A | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | S0,5 | 2.6 | 0.1 | 0.12 | 0.03 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | unstable mutants | no>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
211 | 11747307 | Saccharomyces cerevisiae | mutant R369A | 11747307_Fenton_ArchBiochemBiophys_vol397_pp28-39_2002 | nHill | 3.7 | 0.1 | 1.3 | 0.5 | 6.2 | 25 | 110 mM Mes, 113 mM KCl, 55 mM (NH4)2SO4, 28mM MgCl2, 10 U/ml L-lactic dehydrogenase, 300 mM β-NADH, 5.9 mM ADP, + - 2.4 mM FBP | unstable mutants | no>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||
212 | 1200735 | Alcaligenes eutrophus H 16 | ATCC 17699 | Wilke_ArchMicrobiol_vol105_pp109-15_1975 | 1,6 mM | S0,5 | 0.12 | 3.2 | 0.446 | 0.166 | 7.4 | 30 | 50 mM tris (hydroxymethyl)-aminomethane (TRIS)-HCl, 6 mM MgSO4, 0.23 mM NADH, 0.7 mM ADP, 7.15 U/ml LDH, 9 mM ortho-phosphate; 3.5 µg of the purified enzyme | G6P>R5P>AMP>no | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
213 | 1200735 | Alcaligenes eutrophus H 16 | ATCC 17699 | Wilke_ArchMicrobiol_vol105_pp109-15_1975 | 1,6 mM | nHill | 0.96 | 1.44 | 1.52 | 1.3 | 7.4 | 30 | 50 mM tris (hydroxymethyl)-aminomethane (TRIS)-HCl, 6 mM MgSO4, 0.23 mM NADH, 0.7 mM ADP, 7.15 U/ml LDH, 9 mM ortho-phosphate; 3.5 µg of the purified enzyme | R5P>G6P>AMP>no | nHill | ||||||||||||||||||||||||||||||||||||||||||
214 | 1371328 | Trypanosoma brucei | Mg2+ dependent | Callens_MolBiochemParasit_vol50_pp235-44_1992 | S0,5 | 1.48 | 0.46 | 0.15 | ribulose 1,5-bisphosphate [R15P]:0.40; 5-phosphorylribose 1-pyrophosphate[5PR1P]:0.75; G16P:0.44 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 50 mM KCl, 0.42 mM NADH, 6.25 µg lactate dehydrogenase | no>5PR1P>FBP>G16P>R15P>F26P | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
215 | 1371328 | Trypanosoma brucei | Mg2+ dependent | Callens_MolBiochemParasit_vol50_pp235-44_1992 | nHill | 2.4 | 1.4 | 1 | ribulose 1,5-bisphosphate [R15P]:1.5; 5-phosphorylribose 1-pyrophosphate[5PR1P]:2.0; G16P:1.3 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 50 mM KCl, 0.42 mM NADH, 6.25 µg lactate dehydrogenase | no>5PR1P>R15P>FBP>G16P>F26P | nHill | |||||||||||||||||||||||||||||||||||||||||||
216 | 1371328 | Trypanosoma brucei | Co2+ dependent | Callens_MolBiochemParasit_vol50_pp235-44_1992 | S0,5 | 3.89 | 0.76 | 1.07 | ribulose 1,5-bisphosphate [R15P]:0.23; 5-phosphorylribose 1-pyrophosphate[5PR1P]:0.57; G16P:0.37 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 50 mM KCl, 0.42 mM NADH, 6.25 µg lactate dehydrogenase | no>F26P>FBP>5PR1P>G16P>R15P | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
217 | 1371328 | Trypanosoma brucei | Co2+ dependent | Callens_MolBiochemParasit_vol50_pp235-44_1992 | nHill | 1.2 | 1 | 1 | ribulose 1,5-bisphosphate [R15P]:0.95; 5-phosphorylribose 1-pyrophosphate[5PR1P]:0.95; G16P:1.0 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 50 mM KCl, 0.42 mM NADH, 6.25 µg lactate dehydrogenase | no>F26P=FBP=G16P>5PR1P=R15P | nHill | |||||||||||||||||||||||||||||||||||||||||||
218 | 1371328 | Trypanosoma brucei | Mn2+ dependent | Callens_MolBiochemParasit_vol50_pp235-44_1992 | S0,5 | 0.28 | 0.38 | 0.07 | ribulose 1,5-bisphosphate [R15P]:0.07; 5-phosphorylribose 1-pyrophosphate[5PR1P]:0.13; G16P:0.11 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 50 mM KCl, 0.42 mM NADH, 6.25 µg lactate dehydrogenase | FBP>no>5PR1P>G16P>F26P=R15P | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
219 | 1371328 | Trypanosoma brucei | Mn2+ dependent | Callens_MolBiochemParasit_vol50_pp235-44_1992 | nHill | 2 | 1.1 | 0.99 | ribulose 1,5-bisphosphate [R15P]:0.99; 5-phosphorylribose 1-pyrophosphate[5PR1P]:1.0; G16P:1.11 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 50 mM KCl, 0.42 mM NADH, 6.25 µg lactate dehydrogenase | no>G16P>FBP>5PR1P>F26P=R15P | nHill | |||||||||||||||||||||||||||||||||||||||||||
220 | 9693062 | Trypanosoma brucei | natural | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | S0,5 | 1.48 | 0.15 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
221 | 9693062 | Trypanosoma brucei | natural | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | nHill | 2.4 | 1 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||||
222 | 9693062 | Trypanosoma brucei | natural | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | kcat [1/s] | 550 | 477 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||||
223 | 9693062 | Trypanosoma brucei | recombinant WT | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | S0,5 | 1.28 | 0.01 | 0.055 | 0.011 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
224 | 9693062 | Trypanosoma brucei | recombinant WT | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | nHill | 3.16 | 0.3 | 1 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | nHill | |||||||||||||||||||||||||||||||||||||||||||
225 | 9693062 | Trypanosoma brucei | recombinant WT | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | kcat [1/s] | 338 | 10.5 | 316 | 5.2 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||
226 | 9693062 | Trypanosoma brucei | recombinant mutant R22G | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | S0,5 | 3.73 | 0.2 | 0.88 | 0.35 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
227 | 9693062 | Trypanosoma brucei | recombinant mutant R22G | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | nHill | 3.18 | 0.14 | 1.46 | 0.64 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||
228 | 9693062 | Trypanosoma brucei | recombinant mutant R22G | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | kcat [1/s] | 225 | 11.2 | 211.2 | 43.3 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>=F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||
229 | 9693062 | Trypanosoma brucei | recombinant mutant R19Q | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | S0,5 | 3.88 | 0.18 | 0.31 | 0.11 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
230 | 9693062 | Trypanosoma brucei | recombinant mutant R19Q | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | nHill | 2.56 | 0.11 | 0.83 | 0.11 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||
231 | 9693062 | Trypanosoma brucei | recombinant mutant R19Q | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0-2 mM | kcat [1/s] | 312.3 | 7 | 267 | 21.5 | 7.2 | 25 | 50 mM triethanolamine, 2 mM ADP, 6 mM MgSO4, 50 mM KCl, 0.42 mM NADH, 6.25 µg LDH, + - 1 µM F26P | no>F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||
232 | 9693062 | Trypanosoma brucei | recombinant WT | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | Ka0,5 | 0.026 | 0.009 | 0.013 | 0.006 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>F26P | Ka0,5 | ||||||||||||||||||||||||||||||||||||||||||
233 | 9693062 | Trypanosoma brucei | recombinant WT | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | nHill | 1 | 1 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP=F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||||
234 | 9693062 | Trypanosoma brucei | recombinant WT | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | kcat [1/s] | 231.2 | 22.8 | 234.8 | 18 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | F26P>=FBP | kcat | ||||||||||||||||||||||||||||||||||||||||||
235 | 9693062 | Trypanosoma brucei | recombinant mutant R22G | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | Ka0,5 | 0.214 | 0.033 | 0.068 | 0.001 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>F26P | Ka0,5 | ||||||||||||||||||||||||||||||||||||||||||
236 | 9693062 | Trypanosoma brucei | recombinant mutant R22G | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | nHill | 1 | 2.11 | 0.39 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | F26P>FBP | nHill | |||||||||||||||||||||||||||||||||||||||||||
237 | 9693062 | Trypanosoma brucei | recombinant mutant R22G | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | kcat [1/s] | 126.2 | 0.5 | 128.8 | 1.3 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | F26P>FBP | kcat | ||||||||||||||||||||||||||||||||||||||||||
238 | 9693062 | Trypanosoma brucei | recombinant mutant L76R | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | Ka0,5 | 0.02 | 0.009 | 0.01 | 0.006 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>F26P | Ka0,5 | ||||||||||||||||||||||||||||||||||||||||||
239 | 9693062 | Trypanosoma brucei | recombinant mutant L76R | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | nHill | 1 | 1 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP=F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||||
240 | 9693062 | Trypanosoma brucei | recombinant mutant L76R | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | kcat [1/s] | 234 | 20.7 | 226.3 | 22.3 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>=F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||
241 | 9693062 | Trypanosoma brucei | recombinant mutant F463V | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | Ka0,5 | 0.054 | 0.013 | 0.046 | 0.014 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>=F26P | Ka0,5 | ||||||||||||||||||||||||||||||||||||||||||
242 | 9693062 | Trypanosoma brucei | recombinant mutant F463V | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | nHill | 1 | 1 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP=F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||||
243 | 9693062 | Trypanosoma brucei | recombinant mutant F463V | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | kcat [1/s] | 172 | 11 | 171.7 | 12 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>=F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||
244 | 9693062 | Trypanosoma brucei | recombinant mutant R19Q | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | Ka0,5 | 0.079 | 0.002 | 0.0142 | 6.0E-4 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>F26P | Ka0,5 | ||||||||||||||||||||||||||||||||||||||||||
245 | 9693062 | Trypanosoma brucei | recombinant mutant R19Q | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | nHill | 0.985 | 1.01 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | F26P>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
246 | 9693062 | Trypanosoma brucei | recombinant mutant R19Q | Ernest_ProtExpresPurific_vol13_pp373-382_1998 REF: Callens_MolBiochemParasit_vol50_pp235-44_1992 | 0,7 mM | kcat [1/s] | 133.3 | 0.7 | 127 | 1 | 7.2 | 25 | 50 mM triethanolamine, 50 mM KCl, 6 mM MgSO4, 0.3 mM ADP | FBP>F26P | kcat | ||||||||||||||||||||||||||||||||||||||||||
247 | 2668108 | Trypanosoma cruzi | epimastigotes, Tulahuén strain | Cazzulo_FEMSMicrobLett_vol59_pp259-64_1989 | 0,3 mM | S0,5 | 0.58 | 0.12 | 1.86 | +Pi: 2.16 | 6.8 | 30 | 100 µM Tris-acetate, 30 µM MgCl2, 105 µM KCl, 0.125 µM NADH, 2 µM ADP, 10 U/ml LDH | Pi>ATP>no>F26P | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
248 | 2668108 | Trypanosoma cruzi | epimastigotes, Tulahuén strain | Cazzulo_FEMSMicrobLett_vol59_pp259-64_1989 | 0,3 mM | nHill | 2.4 | 1.16 | 3.34 | +Pi: 3.05 | 6.8 | 30 | 100 µM Tris-acetate, 30 µM MgCl2, 105 µM KCl, 0.125 µM NADH, 2 µM ADP, 10 U/ml LDH | ATP>Pi>no>F26P | nHill | ||||||||||||||||||||||||||||||||||||||||||
249 | 1518529 | Trichomonas vaginalis | C-I (ATCC- 30001) | Mertens_MolBiohemParasit_vol54_pp13-20_1992 | 1 mM | S0,5 | 0.1 | 0.04 | Glycerate-3-P [G3P]:52 | 7 | 30 | 50 mM Hepes, 2 mM ADP, 5 mM MgCl2, 0.14 mM NADH, 0.5 U/ml lactate dehydrogenase | no>G3P>R5P | S0,5, no Pi | |||||||||||||||||||||||||||||||||||||||||||
250 | 1518529 | Trichomonas vaginalis | C-I (ATCC- 30001) | Mertens_MolBiohemParasit_vol54_pp13-20_1992 | 1 mM | nHill | 1.75 | 1.3 | Glycerate-3-P [G3P]:1.5 | 7 | 30 | 50 mM Hepes, 2 mM ADP, 5 mM MgCl2, 0.14 mM NADH, 0.5 U/ml lactate dehydrogenase | no>G3P>R5P | nHill, no Pi | |||||||||||||||||||||||||||||||||||||||||||
251 | 1518529 | Trichomonas vaginalis | C-I (ATCC- 30001) | Mertens_MolBiohemParasit_vol54_pp13-20_1992 | 1 mM | S0,5 | 0.28 | 0.079 | Glycerate-3-P [G3P]:168 | 7 | 30 | 50 mM Hepes, 2 mM ADP, 5 mM MgCl2, 0.14 mM NADH, 0.5 U/ml lactate dehydrogenase, 1 mM Pi | no>G3P>R5P | S0,5, 1 mM Pi | |||||||||||||||||||||||||||||||||||||||||||
252 | 1518529 | Trichomonas vaginalis | C-I (ATCC- 30001) | Mertens_MolBiohemParasit_vol54_pp13-20_1992 | 1 mM | nHill | 3.3 | 2 | Glycerate-3-P [G3P]:2.3 | 7 | 30 | 50 mM Hepes, 2 mM ADP, 5 mM MgCl2, 0.14 mM NADH, 0.5 U/ml lactate dehydrogenase, 1 mM Pi | no>G3P>R5P | nHill, 1 mM Pi | |||||||||||||||||||||||||||||||||||||||||||
253 | 1518529 | Trichomonas vaginalis | C-I (ATCC- 30001) | Mertens_MolBiohemParasit_vol54_pp13-20_1992 | 1 mM | S0,5 | 0.398 | 0.074 | Glycerate-3-P [G3P]:260 | 7 | 30 | 50 mM Hepes, 2 mM ADP, 5 mM MgCl2, 0.14 mM NADH, 0.5 U/ml lactate dehydrogenase, 5 mM Pi | no>G3P>R5P | S0,5, 5 mM Pi | |||||||||||||||||||||||||||||||||||||||||||
254 | 1518529 | Trichomonas vaginalis | C-I (ATCC- 30001) | Mertens_MolBiohemParasit_vol54_pp13-20_1992 | 1 mM | nHill | 3.6 | 1.9 | Glycerate-3-P [G3P]:3.6 | 7 | 30 | 50 mM Hepes, 2 mM ADP, 5 mM MgCl2, 0.14 mM NADH, 0.5 U/ml lactate dehydrogenase, 5 mM Pi | no=G3P>R5P | nHill, 5 mM Pi | |||||||||||||||||||||||||||||||||||||||||||
255 | 11994161 | Chlamydia trachomatis | Iliffe_MolMicrob_vol44_819-28_2002 | 0–10 mM | Vmax [U/mg] | 48.2 | 1.1 | 67.1 | 1.3 | 45.9 | 1.6 | 47.1 | 0.9 | 44.9 | 0.5 | 46.5 | 0.5 | 53.1 | 1.9 | 7.3 | 25 | 10 mM Tris-HCl, 10 mM MgCl2, 100 mM KCl, 2 mM ADP, 0.2 mM NADH, 10 U/ml LDH, 1 mM effector | Km for different nucleosides was also determined | F26P>R5P>no>G1P>F1P>G6P>F6P | Vmax | ||||||||||||||||||||||||||||||||
256 | 11994161 | Chlamydia trachomatis | Iliffe_MolMicrob_vol44_819-28_2002 | 0–10 mM | S0,5 | 3.1 | 0.1 | 0.2 | 0.05 | 2.5 | 0.1 | 2.7 | 0.1 | 2.4 | 0.1 | 2.4 | 0.1 | 2.4 | 0.2 | 7.3 | 25 | 10 mM Tris-HCl, 10 mM MgCl2, 100 mM KCl, 2 mM ADP, 0.2 mM NADH, 10 U/ml LDH, 1 mM effector | Km for different nucleosides was also determined | no>G1P>G6P>R5P>=F6P=F1P>>F26P | S0,5 | ||||||||||||||||||||||||||||||||
257 | 11994161 | Chlamydia trachomatis | Iliffe_MolMicrob_vol44_819-28_2002 | 0–10 mM | n | 2.7 | 0.2 | 0.9 | 0.2 | 1.9 | 0.2 | 2 | 0.1 | 2.4 | 0.1 | 2.2 | 0.1 | 1.7 | 0.2 | 7.3 | 25 | 10 mM Tris-HCl, 10 mM MgCl2, 100 mM KCl, 2 mM ADP, 0.2 mM NADH, 10 U/ml LDH, 1 mM effector | Km for different nucleosides was also determined | no>F6P>F1P>G1P>G6P>R5P>F26P | n | ||||||||||||||||||||||||||||||||
258 | 16661357 | Ricinus communis | L., var. Baker 296; cytosolic | Ireland_PlantPhysiol_vol65_pp1188-93_1980 | 0,5 mM | Km | 0.03 | UDP=0.12; GDP=0.25; IDP=0.25 | 7.9 | 25 | 50 mM Tes- NaOH, 1 mm ADP, 10 mM MgCl2, 40 mM KCI, 0.1 mM NADH, 4 units of lactate dehydrogenase | IDP=GDP>UDP>ADP | Km | ||||||||||||||||||||||||||||||||||||||||||||
259 | 16661357 | Ricinus communis | L., var. Baker 296; plastidal | Ireland_PlantPhysiol_vol65_pp1188-93_1980 | 0,5 mM | Km/ S0,5 | 0.25 | UDP=2.40; GDP=2.2; IDP=4.0 (for these: S0,5) | 7.9 | 25 | 50 mM Tes- NaOH, 1 mm ADP, 10 mM MgCl2, 40 mM KCI, 0.1 mM NADH, 4 units of lactate dehydrogenase | ||||||||||||||||||||||||||||||||||||||||||||||
260 | 2563644 | Selenastrum minutum | Naeg. Collins; plastidal | Turpin_ArchBiochemBiophys_vol269_pp228-38_1989 | 2 mM | PEP0,5 | 0.18 | dihydroxyacetone phosphate (DHAP)=0.10 | 6.5 | 25 | 50 mM imidazole-HCl, 2 mM ADP, 10 mM MgCl2, 30 mM KCl, 0.15 mM NADH, and 2.5 units/ml desalted rabbit muscle LDH in a final volume of 1 ml | none>DHAP | PEP0,5 | ||||||||||||||||||||||||||||||||||||||||||||
261 | 2563644 | Selenastrum minutum | Naeg. Collins; plastidal | Turpin_ArchBiochemBiophys_vol269_pp228-38_1989 | 2 mM | nHill | 1.2 | dihydroxyacetone phosphate (DHAP)=1.5 | 6.5 | 25 | 50 mM imidazole-HCl, 2 mM ADP, 10 mM MgCl2, 30 mM KCl, 0.15 mM NADH, and 2.5 units/ml desalted rabbit muscle LDH in a final volume of 1 ml | DHAP>none | nHill | ||||||||||||||||||||||||||||||||||||||||||||
262 | 2563644 | Selenastrum minutum | Naeg. Collins; cytosolic | Turpin_ArchBiochemBiophys_vol269_pp228-38_1989 | 2 mM | PEP0,5 | 0.09 | dihydroxyacetone phosphate (DHAP)=0.06; Glu=0.20; Pi=0.37 | 6.5 | 25 | 50 mM imidazole-HCl, 2 mM ADP, 10 mM MgCl2, 30 mM KCl, 0.15 mM NADH, and 2.5 units/ml desalted rabbit muscle LDH in a final volume of 1 ml | Pi>Glu>none>DAHP | PEP0,5 | ||||||||||||||||||||||||||||||||||||||||||||
263 | 2563644 | Selenastrum minutum | Naeg. Collins; cytosolic | Turpin_ArchBiochemBiophys_vol269_pp228-38_1989 | 2 mM | nHill | 1 | dihydroxyacetone phosphate (DHAP)=1.3; Glu=0.9; Pi=1.5 | 6.5 | 25 | 50 mM imidazole-HCl, 2 mM ADP, 10 mM MgCl2, 30 mM KCl, 0.15 mM NADH, and 2.5 units/ml desalted rabbit muscle LDH in a final volume of 1 ml | Pi>DAHP>none>Glu | nHill | ||||||||||||||||||||||||||||||||||||||||||||
264 | 16049677 | Ricinus communis | L., var. Baker 296; cytosolic | Turner_Planta_vo222_pp1051-62_2005 | 2 mM | Vmax [U/mg] | 79.1 | Glutamate=31.0 | 6.4 | 25 | 50 mM HEPES-KOH, 25 mM KCl, 12 mM MgCl2, 1 mM ADP, 1 mM DTT, 5% (w/v) PEG 8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase in a final volume of 250 µl | none>Glutamate | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
265 | 16049677 | Ricinus communis | L., var. Baker 296; cytosolic | Turner_Planta_vo222_pp1051-62_2005 | 2 mM | Km | 0.082 | Glutamate=0.150 | 6.4 | 25 | 50 mM HEPES-KOH, 25 mM KCl, 12 mM MgCl2, 1 mM ADP, 1 mM DTT, 5% (w/v) PEG 8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase in a final volume of 250 µl | Glutamate>none | Km | ||||||||||||||||||||||||||||||||||||||||||||
266 | 16049677 | Ricinus communis | L., var. Baker 296; cytosolic | Turner_Planta_vo222_pp1051-62_2005 | 2 mM | Vmax [U/mg] | 77.2 | Glutamate=51.0 | 7.4 | 25 | 50 mM HEPES-KOH, 25 mM KCl, 12 mM MgCl2, 1 mM ADP, 1 mM DTT, 5% (w/v) PEG 8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase in a final volume of 250 µl | none>Glutamate | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
267 | 16049677 | Ricinus communis | L., var. Baker 296; cytosolic | Turner_Planta_vo222_pp1051-62_2005 | 2 mM | Km | 0.09 | Glutamate=0.101 | 7.4 | 25 | 50 mM HEPES-KOH, 25 mM KCl, 12 mM MgCl2, 1 mM ADP, 1 mM DTT, 5% (w/v) PEG 8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase in a final volume of 250 µl | Glutamate>none | Km | ||||||||||||||||||||||||||||||||||||||||||||
268 | 16049677 | Ricinus communis | L., var. Baker 296; cytosolic | Turner_Planta_vo222_pp1051-62_2005 | 0,1 mM | I50 [mM] | 8.4 | 8.8 | Glutamate=2.5; PPi=9.8; Citrate=9.2 | 6.4 | 25 | 50 mM HEPES-KOH, 25 mM KCl, 15 mM MgCl2, 0,1 mM ADP, 1 mM DTT, 5% (w/v) PEG 8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase in a final volume of 250 µl | PPi>Citrate>ATP>FBP>Glutamate | I50 | |||||||||||||||||||||||||||||||||||||||||||
269 | 16049677 | Ricinus communis | L., var. Baker 296; cytosolic | Turner_Planta_vo222_pp1051-62_2005 | 0,1 mM | I50 [mM] | 8 | 13.7 | Glutamate=1.2; PPi=17.2; Citrate=14.2 | 7.4 | 25 | 50 mM HEPES-KOH, 25 mM KCl, 15 mM MgCl2, 0,1 mM ADP, 1 mM DTT, 5% (w/v) PEG 8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase in a final volume of 250 µl | PPi>Citrate>ATP>FBP>Glutamate | I50 | |||||||||||||||||||||||||||||||||||||||||||
270 | 10880971 | Brassica napus | cytosolic PYK | Smith_EurJBiochem_vol267_pp4477-85_2000 | 2 mM | Vmax [U/mg] | 51 | L-Asp=71; L-Glu=28.6; Quercetin=22.4 | 6.8 | 24 | 25 mM Mes-25 mM Bistris-propane, 1 mM MgADP, 1 mM dithiothreitol, 5% (w/v) poly(ethylene glycol) 8000, 50 mM KCl, 10 mM MgCl2, 0.15 mM NADH, and 2 U/mL desalted rabbit muscle lactate dehydrogenase | L-Asp>none>L-Glu>Quercentin | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
271 | 10880971 | Brassica napus | cytosolic PYK | Smith_EurJBiochem_vol267_pp4477-85_2000 | 2 mM | Vmax [U/mg] | 43 | L-Asp=60; L-Glu=15.4 | 7.4 | 24 | 25 mM Mes-25 mM Bistris-propane, 1 mM MgADP, 1 mM dithiothreitol, 5% (w/v) poly(ethylene glycol) 8000, 50 mM KCl, 10 mM MgCl2, 0.15 mM NADH, and 2 U/mL desalted rabbit muscle lactate dehydrogenase | L-Asp>none>L-Glu | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
272 | 10880971 | Brassica napus | cytosolic PYK | Smith_EurJBiochem_vol267_pp4477-85_2000 | 2 mM | Km | 0.12 | L-Asp=0.097; L-Glu=0.20; Quercetin=0.33 | 6.8 | 24 | 25 mM Mes-25 mM Bistris-propane, 1 mM MgADP, 1 mM dithiothreitol, 5% (w/v) poly(ethylene glycol) 8000, 50 mM KCl, 10 mM MgCl2, 0.15 mM NADH, and 2 U/mL desalted rabbit muscle lactate dehydrogenase | Quercentin>L-Glu>none>L-Asp | Km | ||||||||||||||||||||||||||||||||||||||||||||
273 | 10880971 | Brassica napus | cytosolic PYK | Smith_EurJBiochem_vol267_pp4477-85_2000 | 2 mM | Km | 0.078 | L-Asp=0.074; L-Glu=0.28 | 7.4 | 24 | 25 mM Mes-25 mM Bistris-propane, 1 mM MgADP, 1 mM dithiothreitol, 5% (w/v) poly(ethylene glycol) 8000, 50 mM KCl, 10 mM MgCl2, 0.15 mM NADH, and 2 U/mL desalted rabbit muscle lactate dehydrogenase | L-Glu>none>L-Asp | Km | ||||||||||||||||||||||||||||||||||||||||||||
274 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | nHill | 1.81 | 1.06 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM ADP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
275 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | K0,5 [mM] | 0.26 | 0.056 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM ADP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
276 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | nHill | 2 | 0.83 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM GDP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
277 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | K0,5 [mM] | 0.24 | 0.07 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM GDP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
278 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | nHill | 1.5 | 0.71 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM IDP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
279 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | K0,5 [mM] | 0.15 | 0.055 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM IDP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
280 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | nHill | 2.11 | 0.72 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM UDP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
281 | 7115773 | Rattus rattus | lung, M2 | Schering_BiochimBiophysActa_vol717_pp337-47_1982 | 1 mM | K0,5 [mM] | 0.21 | 0.045 | 7.1 | 37 | 20 mM potassium phosphate, 5 mM MgCl2, 60 mM KCl, 0.3 mM NADH, 1 mM UDP and 3.8 U lactate dehydrogenase per ml, +/- 0.2 mM FBP | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
282 | 7436410 | Trypanosoma brucei | Flynn_ArchBiochemBiophys_vol200_pp401-409_1980 | 1,67 mM | Km | 0.082 | 0,005 | GDP=(0.098+/-0.010);UDP=(0.410+/-0.130); CDP=(2.53+/-0.64) | 7.4 | 24,4-25,6 | 100 µmol triethanolamine hydrochloride, 0.4 µmol NADH, lactate dehydrogenase 5-10 IU, 200 µmol KCl, 20 µmol MgSO4, 1.25 µmol ADP/ GDP/ UDP/ CDP in a total assay volume of 3.0 ml. | CDP>UDP>GDP>ADP | Km | ||||||||||||||||||||||||||||||||||||||||||||
283 | 7436410 | Trypanosoma brucei | Flynn_ArchBiochemBiophys_vol200_pp401-409_1980 | 1,67 mM | nHill | 2.02 | 0.04 | 0.98 | 0.03 | 7.4 | 24,4-25,6 | 100 µmol triethanolamine hydrochloride, 0.4 µmol NADH, lactate dehydrogenase 5-10 IU, 200 µmol KCl, 20 µmol MgSO4, 1.25 µmol ADP, +/- 1 mM FBP in a total assay volume of 3.0 ml. | none>FBP | nHill | |||||||||||||||||||||||||||||||||||||||||||
284 | 7436410 | Trypanosoma brucei | Flynn_ArchBiochemBiophys_vol200_pp401-409_1980 | 1,67 mM | S0,5 [mM] | 1.34 | 0.05 | 0.25 | 0.02 | 7.4 | 24,4-25,6 | 100 µmol triethanolamine hydrochloride, 0.4 µmol NADH, lactate dehydrogenase 5-10 IU, 200 µmol KCl, 20 µmol MgSO4, 1.25 µmol ADP, +/- 1 mM FBP in a total assay volume of 3.0 ml. | none>FBP | S0,5 | |||||||||||||||||||||||||||||||||||||||||||
285 | 11104698 | Musa cavendishii | L. cv. Cavendish, cytosolic | Turner_BiocemJ_vol352_pp875-82_2000_banana | 2 mM | Km | 0.121 | UDP=0.22; IDP=1.6; GDP=1.3;CDP=4.5 | 6.9 | 25 | 50 mM Hepes/ KOH, 25 mM KCl, 10 mM MgCl2, 1 mM XDP, 1 mM DTT, 5% (w/v) PEG-8000, 0.15 mM NADH and 2 units/ml desalted lactate dehydrogenase, in a final volume of 250 µl. | CDP>IDP>GDP>UDP>ADP | Km | ||||||||||||||||||||||||||||||||||||||||||||
286 | 11104698 | Musa cavendishii | L. cv. Cavendish, cytosolic | Turner_BiocemJ_vol352_pp875-82_2000_banana | 0,1 mM | IC50 [mM] | 9.3 | 4.8 | citrate=8.0; isocitrate=8.1; L-Glu=5.3; L-Malate=38.0; 3-Phosphoglycerate=11.0 | 6.9 | 25 | 50 mM Hepes/ KOH, 25 mM KCl, 10 mM MgCl2, 0.1 mM ADP, 1 mM DTT, 5% (w/v) PEG-8000, 0.15 mM NADH, 5 mM effector (except c(AMP)=0,5 mM) and 2 units/ml desalted lactate dehydrogenase, in a final volume of 250 µl. | L-Malate>3-Phosphoglycerate>FBP>isocitrate>citrate>L-Glu>ATP | IC50 | |||||||||||||||||||||||||||||||||||||||||||
287 | 11104698 | Musa cavendishii | L. cv. Cavendish, cytosolic | Turner_BiocemJ_vol352_pp875-82_2000_banana | 0,1 mM | IC50 [mM] | 18 | 5.5 | citrate=16.0; isocitrate=14.0; L-Glu=2.2; L-Malate=39.0; 3-Phosphoglycerate=34.0 | 7.5 | 25 | 50 mM Hepes/ KOH, 25 mM KCl, 10 mM MgCl2, 0.1 mM ADP, 1 mM DTT, 5% (w/v) PEG-8000, 0.15 mM NADH, 5 mM effector (except c(AMP)=0.5 mM) and 2 units/ml desalted lactate dehydrogenase, in a final volume of 250 µl. | L-Malate>3-Phosphoglycerate>FBP>citrate>isocitrate>ATP>L-Glu | IC50 | |||||||||||||||||||||||||||||||||||||||||||
288 | 15567170 | Plasmodium falciparum | Tan strain; recombinant, expressed in E.coli | Chan_BiochemBiophysResCom_vol326_pp188-96_2005 | 0,1-2 mM | Km | 0.19 | 0.028 | 0.214 | 0.004 | 0.143 | 0.03 | 0.497 | 0.074 | citrate=(0.507+/-0.069) | 7.5 | 26 | 10 mM Hepes,10 mM MgCl2, 50 mM KCl, 0.5 mM NADH, 3 mM ADP, 22 U LDH in 200 µl | c(ATP)=2 mM; c(G6P),c(FBP) and c(citrate)= 1 mM | citrate>ATP>FBP>G6P>none | Km | ||||||||||||||||||||||||||||||||||||
289 | 15567170 | Plasmodium falciparum | Tan strain; recombinant, expressed in E.coli | Chan_BiochemBiophysResCom_vol326_pp188-96_2005 | 0,1-2 mM | Vmax [U/mg] | 276 | 28 | 291 | 26 | 326 | 15 | 246 | 17 | citrate=(275+/-28) | 7.5 | 26 | 10 mM Hepes,10 mM MgCl2, 50 mM KCl, 0.5 mM NADH, 3 mM ADP, 22 U LDH in 200 µl | c(ATP)=2 mM; c(G6P),c(FBP) and c(citrate)= 1 mM | G6P>FBP>none>citrate>ATP | Vmax | ||||||||||||||||||||||||||||||||||||
290 | 15567170 | Plasmodium falciparum | Tan strain; recombinant, expressed in E.coli | Chan_BiochemBiophysResCom_vol326_pp188-96_2005 | 0,1-2 mM | Km | 0.337 | 0.031 | 0.307 | 0.07 | 0.392 | 0.076 | 7.5 | 26 | 10 mM Hepes,10 mM MgCl2, 50 mM KCl, 0.5 mM NADH, 1 mM ADP, 22 U LDH in 200 µl | c(G6P),c(FBP)=1 mM | G6P>none>FBP | Km | |||||||||||||||||||||||||||||||||||||||
291 | 15567170 | Plasmodium falciparum | Tan strain; recombinant, expressed in E.coli | Chan_BiochemBiophysResCom_vol326_pp188-96_2005 | 0,1-2 mM | Vmax [U/mg] | 96.8 | 9.4 | 86.8 | 4.7 | 121.6 | 12.3 | 7.5 | 26 | 10 mM Hepes,10 mM MgCl2, 50 mM KCl, 0.5 mM NADH, 1 mM ADP, 22 U LDH in 200 µl | c(G6P),c(FBP)=1 mM | G6P>none>FBP | Vmax | |||||||||||||||||||||||||||||||||||||||
292 | 11913971 | Brassica napus | plastidic | Plaxton_ArchBiochemBiophys_vol400_pp54-62_2002 | 2 mM | Vmax [U/mg] | 29.5 | 6-P gluconate=39.8; 2-oxoglutarate=20.8 | 8 | 24 | 50 mM Hepes-KOH, 1 mM ADP, 1 mM dithiothreitol, 5% (w/v) PEG (averageMr 8 kDa), 50 mM KCl, 15 mM MgCl2, 0.15 mM NADH, and 2.5 units/ml of desalted rabbit muscle lactate dehydrogenase; final volume: 1 mL | c(6-p gluconate)=0,5 mM; c(2-oxoglutarate)=8mM | 6-P gluconate>none>2-oxoglutarate | Vmax | |||||||||||||||||||||||||||||||||||||||||||
293 | 11913971 | Brassica napus | plastidic | Plaxton_ArchBiochemBiophys_vol400_pp54-62_2002 | 2 mM | Km | 0.052 | 6-P gluconate=0.029; 2-oxoglutarate=0.24 | 8 | 24 | 50 mM Hepes-KOH, 1 mM ADP, 1 mM dithiothreitol, 5% (w/v) PEG (averageMr 8 kDa), 50 mM KCl, 15 mM MgCl2, 0.15 mM NADH, and 2.5 units/ml of desalted rabbit muscle lactate dehydrogenase; final volume: 1 mL | c(6-p gluconate)=0,5 mM; c(2-oxoglutarate)=8mM | 2-oxoglutarate>none>6-P gluconate | Km | |||||||||||||||||||||||||||||||||||||||||||
294 | 3297035 | Salmonella typhimurium | strain LT-2 | Garcia-Olalla_BiochemJ_vol241_pp573-81_1987 | 1 µM | nHill | 3 | 1 | 6.8 | 30 | 30 µM Hepes, 5 µM MgCl2, 0.15 µM NADH, 2 µM ADP, +/- 1 µM FBP, 5 µg LDH, total volume: 1 mL | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
295 | 3297035 | Salmonella typhimurium | strain LT-2 | Garcia-Olalla_BiochemJ_vol241_pp573-81_1987 | 1 µM | S0,5 | 4.5 | 0.21 | 6.8 | 30 | 30 µM Hepes, 5 µM MgCl2, 0.15 µM NADH, 2 µM ADP, +/- 1 µM FBP, 5 µg LDH, total volume: 1 mL | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
296 | 2503406 | Entodinium sp. | rumen ciliates; PYK type I | Wakita_IntJBiochem_vol21_pp77-81_1989 | 1 mM | Km | 0.15 | 0.28 | 5.75 | 37 | 10 mM phosphate buffer, 1 mM ADP, 10 mM MgSO4, 10 mM KCl, +/- 1 mM FBP; total volume: 0.4 mL | FBP>none | Km | ||||||||||||||||||||||||||||||||||||||||||||
297 | 2503406 | Entodinium sp. | rumen ciliates; PYK type II | Wakita_IntJBiochem_vol21_pp77-81_1989 | 1 mM | Km | 1 | 1 | 6 | 37 | 10 mM phosphate buffer, 1 mM ADP, 10 mM MgSO4, 10 mM KCl, +/- 1 mM FBP; total volume: 0.4 mL | FBP=none | Km | ||||||||||||||||||||||||||||||||||||||||||||
298 | 2503406 | Entodinium sp. | rumen ciliates; PYK type III | Wakita_IntJBiochem_vol21_pp77-81_1989 | 1 mM | Km | 0.24 | 0.24 | 5.75 | 37 | 10 mM phosphate buffer, 1 mM ADP, 10 mM MgSO4, 10 mM KCl, +/- 1 mM FBP; total volume: 0.4 mL | FBP=none | Km | ||||||||||||||||||||||||||||||||||||||||||||
299 | BrendaID641532 | Cardium tuberculatum | foot muscle | Chrispeels_CompBiochemPhysiol_vol82B_pp163-72_1985 | 0,5 mM | Km | 0.07 | 0.04 | 7.2 | 25 | 50 mM imidazole/HCl, 2mM ADP, 80mM KCl, 3mM MgSO4, 0.15mM NADH, +/- 0.5 mM FBP and 5.5 U LDH in a final volume of 1 ml | none>FBP | Km | ||||||||||||||||||||||||||||||||||||||||||||
300 | BrendaID641532 | Cardium tuberculatum | foot muscle | Chrispeels_CompBiochemPhysiol_vol82B_pp163-72_1985 | 0,5 mM | nHill | 1.2 | 1.6 | 7.2 | 25 | 50 mM imidazole/HCl, 2mM ADP, 80mM KCl, 3mM MgSO4, 0.15mM NADH, +/- 50 mM L-Ala and 5.5 U LDH in a final volume of 1 ml | L-Ala>none | nHill | ||||||||||||||||||||||||||||||||||||||||||||
301 | BrendaID641532 | Cardium tuberculatum | foot muscle | Chrispeels_CompBiochemPhysiol_vol82B_pp163-72_1985 | 0,5 mM | Km/ S0,5 | 0.07 | 0.56 | 7.2 | 25 | 50 mM imidazole/HCl, 2mM ADP, 80mM KCl, 3mM MgSO4, 0.15mM NADH, +/- 50 mM L-Ala and 5.5 U LDH in a final volume of 1 ml | L-Ala>none | Km/ S0,5 | ||||||||||||||||||||||||||||||||||||||||||||
302 | BrendaID641542 | Nautilus pompilius | retractor muscle | Fields_PacificScience_vol36_1982 | 2 mM | Km | 0.06 | 0.006 | 0.057 | 0.009 | 0.28 | 0.02 | 7 | 25 | 100 mM imidazole-HCI, 4 mM ADP, 10mM MgS04 , 100mM KCI, 0.2 mM NADH, 5 units of beef heart lactate dehydrogenase | c(FBP)=0,5 mM; c(Ala)=50mM | Ala>none=FBP | Km | |||||||||||||||||||||||||||||||||||||||
303 | BrendaID641542 | Nautilus pompilius | spadix muscle | Fields_PacificScience_vol36_1982 | 2 mM | S0,5 | 0.063 | 0.061 | 0.4 | 7 | 25 | 100 mM imidazole-HCI, 4 mM ADP, 10mM MgS04 , 100mM KCI, 0.2 mM NADH, 5 units of beef heart lactate dehydrogenase | c(FBP)=0,5 mM; c(Ala)=5 mM | Ala>none=FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
304 | 6468395 | Busycotypus canaliculatum | from aerobic animals | Plaxton_EurJBiochem_vol143_pp257-65_1984 | 1 mM | Ki (app) | 24.5 | 2.5 | 2.68 | 0.13 | 7 | 20 | 50 mM imidazole-HCI, 2 mM ADP, 50 mM KCI, 5 mM MgCl2, 0.15 mM NADH and 2.0 I.U./ml dialyzed lactate dehydrogenase in a final volume of 1.0 ml | L-Ala>ATP | Ki | ||||||||||||||||||||||||||||||||||||||||||
305 | 6468395 | Busycotypus canaliculatum | from anoxic animals | Plaxton_EurJBiochem_vol143_pp257-65_1984 | 5 mM | Ki (app) | 0.05 | 0.004 | 0.78 | 0.08 | 7 | 20 | 50 mM imidazole-HCI, 2 mM ADP, 50 mM KCI, 5 mM MgCl2, 0.15 mM NADH and 2.0 I.U./ml dialyzed lactate dehydrogenase in a final volume of 1.0 ml | ATP>L-Ala | Ki | ||||||||||||||||||||||||||||||||||||||||||
306 | 6468395 | Busycotypus canaliculatum | from aerobic animals | Plaxton_EurJBiochem_vol143_pp257-65_1984 | 0,07 mM | Ka (app) | 0.054 | 0.008 | 12.5 | 3 | 7 | 20 | 50 mM imidazole-HCI, 2 mM ADP, 50 mM KCI, 5 mM MgCl2, 0.15 mM NADH and 2.0 I.U./ml dialyzed lactate dehydrogenase in a final volume of 1.0 ml | F26P>FBP | Ka | ||||||||||||||||||||||||||||||||||||||||||
307 | 6468395 | Busycotypus canaliculatum | from anoxic animals | Plaxton_EurJBiochem_vol143_pp257-65_1984 | 0,07 mM | Ka (app) | 1.3 | 0.1 | 154 | 21 | 7 | 20 | 50 mM imidazole-HCI, 2 mM ADP, 50 mM KCI, 5 mM MgCl2, 0.15 mM NADH and 2.0 I.U./ml dialyzed lactate dehydrogenase in a final volume of 1.0 ml | F26P>FBP | Ka | ||||||||||||||||||||||||||||||||||||||||||
308 | 4062301 | Busycotypus canaliculatum | from aerobic animals | Plaxton_ArchBiochemBiophys_vol243_pp195-205_1985 | 2 mM | Vmax | 18 | 3 | 167 | 11 | Aspartate=233+/-16 | 7 | 20 | 50 mM imidazole-HCl, 2 mM ADP, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, +/- 50 µM FBP, +/- 3 mM Aspartate and 1.5 IU/ ml dialyzed rabbit muscle LDH in a final volume of 1.0 ml | Aspartate>FBP>none | Vmax | |||||||||||||||||||||||||||||||||||||||||
309 | 4062301 | Busycotypus canaliculatum | from anoxic animals | Plaxton_ArchBiochemBiophys_vol243_pp195-205_1985 | 10 mM | Vmax | 2 | 0.2 | 2.4 | 0.3 | Aspartate=2.5+/-0.1 | 7 | 20 | 50 mM imidazole-HCl, 2 mM ADP, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, +/- 50 µM FBP, +/- 3 mM Aspartate and 1.5 IU/ ml dialyzed rabbit muscle LDH in a final volume of 1.0 ml | Aspartate>=FBP>none | Vmax | |||||||||||||||||||||||||||||||||||||||||
310 | 4062301 | Busycotypus canaliculatum | from aerobic animals | Plaxton_ArchBiochemBiophys_vol243_pp195-205_1985 | 2 mM | PEP0,5 | 0.38 | 0.01 | 0.12 | 0.01 | Aspartate=0.17+/-0.01 | 7 | 20 | 50 mM imidazole-HCl, 2 mM ADP, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, +/- 50 µM FBP, +/- 3 mM Aspartate and 1.5 IU/ ml dialyzed rabbit muscle LDH in a final volume of 1.0 ml | none>Aspartate>FBP | PEP0,5 | |||||||||||||||||||||||||||||||||||||||||
311 | 4062301 | Busycotypus canaliculatum | from anoxic animals | Plaxton_ArchBiochemBiophys_vol243_pp195-205_1985 | 10 mM | PEP0,5 | 1.1 | 0.1 | 0.11 | 0.01 | Aspartate=0.28+/-0.01 | 7 | 20 | 50 mM imidazole-HCl, 2 mM ADP, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, +/- 50 µM FBP, +/- 3 mM Aspartate and 1.5 IU/ ml dialyzed rabbit muscle LDH in a final volume of 1.0 ml | none>Aspartate>FBP | PEP0,5 | |||||||||||||||||||||||||||||||||||||||||
312 | 4062301 | Busycotypus canaliculatum | from aerobic animals | Plaxton_ArchBiochemBiophys_vol243_pp195-205_1985 | 2 mM | nHill | 1.15 | 0.09 | 1.05 | 0.03 | Aspartate=1.10+/-0.05 | 7 | 20 | 50 mM imidazole-HCl, 2 mM ADP, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, +/- 50 µM FBP, +/- 3 mM Aspartate and 1.5 IU/ ml dialyzed rabbit muscle LDH in a final volume of 1.0 ml | none>=Aspartate>=FBP | nHill | |||||||||||||||||||||||||||||||||||||||||
313 | 4062301 | Busycotypus canaliculatum | from anoxic animals | Plaxton_ArchBiochemBiophys_vol243_pp195-205_1985 | 10 mM | nHill | 1.19 | 0.03 | 0.91 | 0.12 | Aspartate=1.04+/-0.1 | 7 | 20 | 50 mM imidazole-HCl, 2 mM ADP, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, +/- 50 µM FBP, +/- 3 mM Aspartate and 1.5 IU/ ml dialyzed rabbit muscle LDH in a final volume of 1.0 ml | none>Aspartate>=FBP | nHill | |||||||||||||||||||||||||||||||||||||||||
314 | 18775437 | Leishmania mexicana | WT, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | S0,5 | 1.06 | 0.06 | 0.165 | 0.012 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
315 | 18775437 | Leishmania mexicana | WT, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | nHill | 1.55 | 0.14 | 1.26 | 0.069 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||
316 | 18775437 | Leishmania mexicana | mutant S314N, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | S0,5 | 5.11 | 0.271 | 0.496 | 0.022 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
317 | 18775437 | Leishmania mexicana | mutant S314N, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | nHill | 1.5 | 0.103 | 1.91 | 1.12 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | FBP><= none | nHill | ||||||||||||||||||||||||||||||||||||||||||
318 | 18775437 | Leishmania mexicana | mutant S314Q, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | S0,5 | 0.457 | 0.021 | 0.207 | 0.016 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
319 | 18775437 | Leishmania mexicana | mutant S314Q, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | nHill | 1.68 | 0.121 | 1.48 | 1.04 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none><= FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||
320 | 18775437 | Leishmania mexicana | mutant D315N, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | S0,5 | 2.8 | 0.116 | 1.51 | 0.046 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
321 | 18775437 | Leishmania mexicana | mutant D315N, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | nHill | 1.97 | 0.131 | 1.67 | 0.518 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>=FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||
322 | 18775437 | Leishmania mexicana | mutant D315S, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | S0,5 | 3.66 | 0.852 | 2.27 | 0.298 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
323 | 18775437 | Leishmania mexicana | mutant D315S, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | nHill | 1.09 | 0.112 | 1.72 | 2.07 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | FBP><= none | nHill | ||||||||||||||||||||||||||||||||||||||||||
324 | 18775437 | Leishmania mexicana | mutant E451W, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | S0,5 | 1.3 | 0.073 | 0.216 | 0.026 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
325 | 18775437 | Leishmania mexicana | mutant E451W, expressed in E. coli BL21(DE3) cells | JMolBiol_2008_383_615_sulphate-Leishmania_Tulloch | 2,5 mM | nHill | 1.69 | 0.12 | 1.53 | 0.13 | 7.2 | 25 | 50 mM triethanolamine/HCl, 2 mM ADP, 6 mM Mg2SO4, 50 mM KCl, 0.42 mM NADH, +/- 10 µM FBP and 1.6 units of beef heart LDH, 1 mL | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||
326 | 11238975 | Selenomonas ruminantium | TH1 | Asanuma_Microbiol_vol147_pp681-90_2001 | 5 mM | Enzyme activity * | 55 | 4.1 | 117 | 7.5 | Pi=28 +/- 2.2 | 7 | 50 mM Tris/ HCl, 8 mM MgCl2, 80 mM KCl, 5 mM ADP, +/- 0.5 mM FBP OR +/- 0.1 mM Pi, 10 U Ldh, 0.2 mM NADH and cell extracts [1-2 µg protein/ (ml assay mixture)] | µmol NADH oxidized/ (min*(10 µg cellular N)) | FBP>none>Pi | Enzyme activity | |||||||||||||||||||||||||||||||||||||||||
327 | 11238975 | Selenomonas ruminantium | TH1 | Asanuma_Microbiol_vol147_pp681-90_2001 | 5 mM | Enzyme activity * | 55 | 4.1 | 135 | 11 | 7 | 50 mM Tris/ HCl, 8 mM MgCl2, 80 mM KCl, 5 mM ADP, +/- 0.5 mM FBP AND +/- 0.1 mM Pi, 10 U Ldh, 0.2 mM NADH and cell extracts [1-2 µg protein/ (ml assay mixture)] | µmol NADH oxidized/ (min*(10 µg cellular N)) | FBP+Pi>none | Enzyme activity | ||||||||||||||||||||||||||||||||||||||||||
328 | 15996096 | Homo sapiens | hPKM2 | Dombrauckas_Biochem_voll44_pp9417-29_2005 | 5 mM | Km | 2.1 | 0.7 | 0.17 | 0.01 | 8 | 32 | 50 mM Bis Tris Propane, 5 mM ADP, +/- 2 mM FBP, 15 mM MgCl2, 200 mM KCl, and 175 µM NADH, 25% glycerol in 1 ml | none>FBP | Km | ||||||||||||||||||||||||||||||||||||||||||
329 | 15996096 | Homo sapiens | hPKM2 | Dombrauckas_Biochem_voll44_pp9417-29_2005 | 5 mM | Vmax | 310 | 37 | 218 | 4 | 8 | 32 | 50 mM Bis Tris Propane, 5 mM ADP, +/- 2 mM FBP, 15 mM MgCl2, 200 mM KCl, and 175 µM NADH, 25% glycerol in 1 ml | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||
330 | 15996096 | Homo sapiens | hPKM2 | Dombrauckas_Biochem_voll44_pp9417-29_2005 | 5 mM | nHill | 1.6 | 0.1 | 1 | 8 | 32 | 50 mM Bis Tris Propane, 5 mM ADP, +/- 2 mM FBP, 15 mM MgCl2, 200 mM KCl, and 175 µM NADH, 25% glycerol in 1 ml | none>FBP | nHill | |||||||||||||||||||||||||||||||||||||||||||
331 | 11163314 | Entosphenus japonicas | from skeletal muscle | Nishikawa_CompBiochemPhysiol_vol128_pp159-64_2001 | 2 mM | Km | 0.41 | 0.087 | 7.4 | 30 | 50 mM Tris/ HCl, 0.1 M KCl, 5 mM MgCl2 , 2 mM ADP, 2 mM PEP, 0.17 mM NADH and 2 units of LDH; 1 mL | none>FBP | Km | ||||||||||||||||||||||||||||||||||||||||||||
332 | 11163314 | Entosphenus japonicas | from skeletal muscle | Nishikawa_CompBiochemPhysiol_vol128_pp159-64_2001 | 2 mM | Vmax | 4.13E-3 | 4.13E-3 | 7.4 | 30 | 50 mM Tris/ HCl, 0.1 M KCl, 5 mM MgCl2 , 2 mM ADP, 2 mM PEP, 0.17 mM NADH and 2 units of LDH; 1 mL | none=FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
333 | 11163314 | Entosphenus japonicas | from skeletal muscle | Nishikawa_CompBiochemPhysiol_vol128_pp159-64_2001 | 2 mM | nHill | 1.3 | 0.98 | 7.4 | 30 | 50 mM Tris/ HCl, 0.1 M KCl, 5 mM MgCl2 , 2 mM ADP, 2 mM PEP, 0.17 mM NADH and 2 units of LDH; 1 mL | none>FBP | nHill | ||||||||||||||||||||||||||||||||||||||||||||
334 | 3970531 | Rattus rattus | brain | ArchBiochemBiophys_1985_703-13_Srivastava_PEP-ATP-ADP-Phe | 0,125 - 1,25 mM | Km | 0.18 | L-Phe = 2.5 mM | 7.4 | 25 | 67.5 mM Tris-HCl, 6.75 mM MgCl2, 95 mM KCI, 0.28 mM NADH, 4 U LDH, 2.5 mM ADP, 0 - 2 mM L-Phe; 1 mL | L-Phe>none | Km | ||||||||||||||||||||||||||||||||||||||||||||
335 | 6509916 | Rana ridibunda | erythrocytes | Kaloyianni-Dimitriades_CompBiochemPhysiol_vol79B_pp245-50_1984 | 0.1 - 1.0 mM | Km | 0.095 | 0.049 | 7.35 | 25 | +/- 0.5 mM FBP | none>FBP | Km | ||||||||||||||||||||||||||||||||||||||||||||
336 | 15158165 | Rattus rattus | Winstar; brain (cortex) | Feksa_BrainRes_vol1012_pp93-100_2004 | 1 | Km/ Ki | 5 E-5 M | 1E-5 M | Ki(cystine) = 2 +/- 0.5 x 10-3 M | 7.5 | 25 | 0.1 M Tris/HCl, 10 mM MgCl2, 0.16 mM NADH, 75 mM KCl, 5.0 mM ADP, 7 U LDH, 0.1% (v/v) Triton X-100, 10 µl of the mitochondria-free supernatant in a final volume of 0.5 ml | |||||||||||||||||||||||||||||||||||||||||||||
337 | 16147999 | rabbit | WT: muscle, expressed in E. coli | Orla-Hernandez_JBC_vol280_pp37924-9_2005 | 0 - 0.17 | Km | 0.61 | 0.07 | K+ = 0.13 +/- 0.007 | 6 | 25 | 50 mM Mes-Tris, 0.2mM NADH, 3mM Mg2+free, 8 g/ml LDH, 5 to 13 g/ml PK, +/- 90 mM K+ | none>K+ | Km | |||||||||||||||||||||||||||||||||||||||||||
338 | 16147999 | rabbit | WT: muscle, expressed in E. coli | Orla-Hernandez_JBC_vol280_pp37924-9_2005 | 0 - 0.17 | kcat [1/s] | 3.2 | K+ = 1182 | 6 | 25 | 50 mM Mes-Tris, 0.2mM NADH, 3mM Mg2+free, 8 g/ml LDH, 5 to 13 g/ml PK, +/- 90 mM K+ | K+>>none | kcat | ||||||||||||||||||||||||||||||||||||||||||||
339 | 16147999 | rabbit | WT: muscle, expressed in E. coli | Orla-Hernandez_JBC_vol280_pp37924-9_2005 | 0 - 0.17 | Vmax [µmol/(min x mg)] | 0.8 | 0.04 | K+ = 299 +/- 11 | 6 | 25 | 50 mM Mes-Tris, 0.2mM NADH, 3mM Mg2+free, 8 g/ml LDH, 5 to 13 g/ml PK, +/- 90 mM K+ | K+>>none | Vmax | |||||||||||||||||||||||||||||||||||||||||||
340 | 15568816 | yeast | WT | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | Km | 1180 | 220 | 310 | 5 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 20 mM MgCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>FBP | Km | |||||||||||||||||||||||||||||||||||||||||||
341 | 15568816 | yeast | WT | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | kcat [1/s] | 232 | 5 | 226 | 11 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 20 mM MgCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>=FBP | kcat | |||||||||||||||||||||||||||||||||||||||||||
342 | 15568816 | yeast | WT | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | nH | 2.8 | 0.1 | 1 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 20 mM MgCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
343 | 15568816 | yeast | WT | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | Km | 45 | 1 | 21 | 3 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 4 mM MnCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>FBP | Km | |||||||||||||||||||||||||||||||||||||||||||
344 | 15568816 | yeast | WT | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | kcat [1/s] | 58.4 | 0.9 | 66 | 1.8 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 4 mM MnCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | FBP>none | kcat | |||||||||||||||||||||||||||||||||||||||||||
345 | 15568816 | yeast | WT | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | nH | 2.5 | 0.1 | 1 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 4 mM MnCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
346 | 15568816 | yeast | mutant T298C | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | Km | 242 | 7 | 66 | 4 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 4 mM MnCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>FBP | Km | |||||||||||||||||||||||||||||||||||||||||||
347 | 15568816 | yeast | mutant T298C | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | kcat [1/s] | 12.2 | 0.2 | 13.9 | 0.2 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 4 mM MnCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | FBP>none | kcat | |||||||||||||||||||||||||||||||||||||||||||
348 | 15568816 | yeast | mutant T298C | Susan-Resiga_Biochem_vol43_pp15230-45_2004 | 20 | nH | 1.5 | 0.06 | 1 | 6.2 | 100 mM MES, 4% glycerol, 200 mM KCl, 4 mM MnCl2, 5 mM ADP, 175 µM NADH, 20 µg of LDH, and 1-2 µg YPK, +/- 1 mM FBP | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||||
349 | BrendaID641609 | Meganyctiphanes norvegica | PK I (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Km | 0.033 | 0.003 | 0.034 | 0.005 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | FBP>=none | Km | PK I summer | |||||||||||||||||||||||||||||||||||||||||
350 | BrendaID641609 | Meganyctiphanes norvegica | PK I (Winter) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Km | 0.024 | 0.002 | 0.022 | 0.012 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | FBP>=none | Km | PK I winter | |||||||||||||||||||||||||||||||||||||||||
351 | BrendaID641609 | Meganyctiphanes norvegica | PK I (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Vmax | 0.268 | 0.014 | 0.269 | 0.014 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | FBP=none | Vmax | PK I summer | |||||||||||||||||||||||||||||||||||||||||
352 | BrendaID641609 | Meganyctiphanes norvegica | PK I (Winter) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Vmax | 0.225 | 0.014 | 0.225 | 0.023 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | FBP=none | Vmax | PK I winter | |||||||||||||||||||||||||||||||||||||||||
353 | BrendaID641609 | Meganyctiphanes norvegica | PK I (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | nH | 0.91 | 0.22 | 1.12 | 0.23 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | FBP>none | nH | PK I summer | |||||||||||||||||||||||||||||||||||||||||
354 | BrendaID641609 | Meganyctiphanes norvegica | PK I (Winter) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | nH | 1.12 | 0.1 | 1.03 | 0.18 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>=FBP | nH | PK I winter | |||||||||||||||||||||||||||||||||||||||||
355 | BrendaID641609 | Meganyctiphanes norvegica | PK II (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Km | 0.181 | 0.014 | 0.027 | 0.006 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>FBP | Km | PK II summer | |||||||||||||||||||||||||||||||||||||||||
356 | BrendaID641609 | Meganyctiphanes norvegica | PK II (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Km | 0.193 | 0.015 | 0.027 | 0.003 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>FBP | Km | PK II winter | |||||||||||||||||||||||||||||||||||||||||
357 | BrendaID641609 | Meganyctiphanes norvegica | PK II (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Vmax | 0.346 | 0.028 | 0.345 | 0.011 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>=FBP | Vmax | PK II summer | |||||||||||||||||||||||||||||||||||||||||
358 | BrendaID641609 | Meganyctiphanes norvegica | PK II (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | Vmax | 0.336 | 0.012 | 0.328 | 0.007 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>=FBP | Vmax | PK II winter | |||||||||||||||||||||||||||||||||||||||||
359 | BrendaID641609 | Meganyctiphanes norvegica | PK II (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | nH | 1.8 | 0.21 | 0.83 | 0.11 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>FBP | nH | PK II summer | |||||||||||||||||||||||||||||||||||||||||
360 | BrendaID641609 | Meganyctiphanes norvegica | PK II (Summer) | Vetter_CompBiochemPhysiol_vol116A_pp1-10_1997 | 3.3 - 100 mM | nH | 1.5 | 0.12 | 0.92 | 0.06 | 7 | 15 | 500 µl extraction buffer (50 mM Tris/HCl, 60 mM KCl and 4 mM MgSO4), 0.14 µM NADH, 0.32 µM PEP, 10 units LDH, 10 to 50 µl of sample, 0.34 µM ADP | none>FBP | nH | PK II winter | |||||||||||||||||||||||||||||||||||||||||
361 | 8806784 | Ricinus communis L. | leaves; cytosolic PK | Hu_ArchBiochemBiophys_vol333_pp298-307_1996 | 2 | Km | 0.089 | aspartate(10 mM)=0.059; citrate(2 mM)=0.11;glutamate(5 mM)=0.093 | 6.5 | 30 | 25 mM Bis–tris-propane/25 mM Mes–HCl, 1 mM MgADP, 20 mM KCl, 10 mM MgCl2 , 0.15 mM NADH, 1 mM DTT, 5% (w/v) PEG (average Mr 8 kDa), 2 U/ml LDH; V=1mL | citrate>glutamate>none>aspartate | Km | ||||||||||||||||||||||||||||||||||||||||||||
362 | 8806784 | Ricinus communis L. | leaves; cytosolic PK | Hu_ArchBiochemBiophys_vol333_pp298-307_1996 | 2 | Vmax | 52.1 | aspartate(10 mM)=56.3; citrate(2 mM)=42.2;glutamate(5 mM)=45.9 | 6.5 | 30 | 25 mM Bis–tris-propane/25 mM Mes–HCl, 1 mM MgADP, 20 mM KCl, 10 mM MgCl2 , 0.15 mM NADH, 1 mM DTT, 5% (w/v) PEG (average Mr 8 kDa), 2 U/ml LDH; V=1mL | aspartate>none>glutamate>citrate | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
363 | 8806784 | Ricinus communis L. | leaves; cytosolic PK | Hu_ArchBiochemBiophys_vol333_pp298-307_1996 | 2 | Km | 0.033 | aspartate(10 mM)=0.026; citrate(2 mM)=0.061;glutamate(5 mM)=0.048 | 7.5 | 30 | 25 mM Bis–tris-propane/25 mM Mes–HCl, 1 mM MgADP, 20 mM KCl, 10 mM MgCl2 , 0.15 mM NADH, 1 mM DTT, 5% (w/v) PEG (average Mr 8 kDa), 2 U/ml LDH; V=1mL | citrate>glutamate>none>aspartate | Km | ||||||||||||||||||||||||||||||||||||||||||||
364 | 8806784 | Ricinus communis L. | leaves; cytosolic PK | Hu_ArchBiochemBiophys_vol333_pp298-307_1996 | 2 | Vmax | 42.6 | aspartate(10 mM)=42.6; citrate(2 mM)=35.1;glutamate(5 mM)=30.9 | 7.5 | 30 | 25 mM Bis–tris-propane/25 mM Mes–HCl, 1 mM MgADP, 20 mM KCl, 10 mM MgCl2 , 0.15 mM NADH, 1 mM DTT, 5% (w/v) PEG (average Mr 8 kDa), 2 U/ml LDH; V=1mL | aspartate=none>citrate>glutamate | Vmax | ||||||||||||||||||||||||||||||||||||||||||||
365 | 7462968 | Cancer magister | muscle | Guderley_JExpZool_vol212_pp461-9_1980 | 2 | Km | 8.5E-5 M | 4.5E-5 M | 7 | 20 | 50 mM imidazole- HCl, 2 mM ADP, 75 mM KCl, 4 mM MgCl2, 0.1 mM NADH, LDH, +/- 0.5 mM FBP | none>FBP | Km | ||||||||||||||||||||||||||||||||||||||||||||
366 | 6614924 | Mycobacterium smegmatis | strain CDC 46 | Kapoor_ArchBiochem_vol255_pp320-30_1983 | 1 | nH | 1.55 | 1.1 | 2.47 | G6P + ATP = 1.65 | 7 | 28 +/- 1 | 50 mM Hepes, 15 mM Mg2+, 2 mM ADP, 0.15 mM NADH, 28 µg LDH, 8-10 µg enzyme preparation, +/- 1 mM G6P and/or 2 mM ATP | ATP>ATP+G6P>none>G6P | nH | ||||||||||||||||||||||||||||||||||||||||||
367 | 6614924 | Mycobacterium smegmatis | strain CDC 46 | Kapoor_ArchBiochem_vol255_pp320-30_1983 | 1 | PEP0,5 | 0.13 | 0.11 | 0.23 | G6P + ATP = 0.12 | 7 | 28 +/- 1 | 50 mM Hepes, 15 mM Mg2+, 2 mM ADP, 0.15 mM NADH, 28 µg LDH, 8-10 µg enzyme preparation, +/- 1 mM G6P and/or 2 mM ATP | ATP>none>ATP+G6P>G6P | PEP0,5 | ||||||||||||||||||||||||||||||||||||||||||
368 | 6614924 | Mycobacterium smegmatis | strain CDC 46 | Kapoor_ArchBiochem_vol255_pp320-30_1983 | 1 | V | 200 | 202 | 96 | G6P + ATP = 111 | 7 | 28 +/- 1 | 50 mM Hepes, 15 mM Mg2+, 2 mM ADP, 0.15 mM NADH, 28 µg LDH, 8-10 µg enzyme preparation, +/- 1 mM G6P and/or 2 mM ATP | G6P>none>ATP+G6P>ATP | V | ||||||||||||||||||||||||||||||||||||||||||
369 | 6614924 | Mycobacterium smegmatis | strain CDC 46 | Kapoor_ArchBiochem_vol255_pp320-30_1983 | 1 | nH | 1.5 | 1 | 2.5 | G6P + ATP = 1.600 | 7 | 28 +/- 1 | 50 mM Hepes, 3 mM Mn2+, 2 mM ADP, 0.15 mM NADH, 28 µg LDH, 8-10 µg enzyme preparation, +/- 1 mM G6P and/or 2 mM ATP | ATP>none>ATP+G6P=G6P | nH | ||||||||||||||||||||||||||||||||||||||||||
370 | 6614924 | Mycobacterium smegmatis | strain CDC 46 | Kapoor_ArchBiochem_vol255_pp320-30_1983 | 1 | PEP0,5 | 0.07 | 0.03 | 0.2 | G6P + ATP = 0.106 | 7 | 28 +/- 1 | 50 mM Hepes, 3 mM Mn2+, 2 mM ADP, 0.15 mM NADH, 28 µg LDH, 8-10 µg enzyme preparation, +/- 1 mM G6P and/or 2 mM ATP | ATP>none>ATP+G6P>G6P | PEP0,5 | ||||||||||||||||||||||||||||||||||||||||||
371 | 6614924 | Mycobacterium smegmatis | strain CDC 46 | Kapoor_ArchBiochem_vol255_pp320-30_1983 | 1 | V | 99.9 | 87.1 | 62.6 | G6P + ATP = 55.4 | 7 | 28 +/- 1 | 50 mM Hepes, 3 mM Mn2+, 2 mM ADP, 0.15 mM NADH, 28 µg LDH, 8-10 µg enzyme preparation, +/- 1 mM G6P and/or 2 mM ATP | none>G6P>ATP>ATP+G6P | V | ||||||||||||||||||||||||||||||||||||||||||
372 | 1426985 | Thermoplasma acidophilum | Potter_FEMSMicrobLett_vol94_pp235-40_1992 | not specified | K0,5 [mM] | 0.64 | 0.7 | 0.043 | 7.5 | 60 | 0.05 M Tris, 7.2 mM MgSO4, 7.2 mM KCl, 1 µg PK, ADP and PEP in invariant concentrations (not specified!) | ATP>none>AMP | K0,5 | ||||||||||||||||||||||||||||||||||||||||||||
373 | 15749828 | Bacillus stearothermophilus | WT | Sakai_JBiochem_vol137_pp141-5_2005 | 2 | Km | 0.186 | 0.03 | 7.2 | 30 | 50 mM imidazole-HCl, 50 mM KCl, 7 mM MgCl2, 0.12 mM NADH, 20 µg/ml LDH, 4 mM ADP, 0.1 mM ribose 5-phosphate | Aminoethylated C9S/C268S/K221C>WT>C9S/C268S | Km | ||||||||||||||||||||||||||||||||||||||||||||
374 | 15749828 | Bacillus stearothermophilus | mutant C9S/C268S, expressed in E. coli | Sakai_JBiochem_vol137_pp141-5_2005 | 2 | Km | 0.113 | 0.009 | 7.2 | 30 | 50 mM imidazole-HCl, 50 mM KCl, 7 mM MgCl2, 0.12 mM NADH, 20 µg/ml LDH, 4 mM ADP, 0.1 mM ribose 5-phosphate | ||||||||||||||||||||||||||||||||||||||||||||||
375 | 15749828 | Bacillus stearothermophilus | mutant Aminoethylated C9S/C268S/K221C, expressed in E. coli | Sakai_JBiochem_vol137_pp141-5_2005 | 2 | Km | 1.23 | 0.107 | 7.2 | 30 | 50 mM imidazole-HCl, 50 mM KCl, 7 mM MgCl2, 0.12 mM NADH, 20 µg/ml LDH, 4 mM ADP, 0.1 mM ribose 5-phosphate | ||||||||||||||||||||||||||||||||||||||||||||||
376 | 3711058 | Bacillus stearothermophilus | strain NCA15O | J Biochem-1986-SAKAI-1157-67 | 0.5 | relative activity | 1 | 0.9 | 1.9 | 1.1 | 4.4 | 23.7 | 24.3 | 3-Phosphoglycerate (3-PG)=2.0; GMP=13.5; IMP=5.8;CMP=4.8 | 7.2 | 30 | 25 µmol imidazole-HCl, 25 µmol KCl, 3.5 µmol MgCl2, 2 µmol ADP, 0.06 µmol NADH, 0.5 mM activator, 10 µg LDH | AMP>R5P>GMP>IMP>CMP>F6P>3-PG>G6P>G1P>none>FBP | relative activity | ||||||||||||||||||||||||||||||||||||||
377 | 3711058 | Bacillus stearothermophilus | strain NCA15O | J Biochem-1986-SAKAI-1157-67 | 0.5 | relative activity | 1 | 0.7 | 1.9 | 0.8 | 1.9 | 24 | 21.7 | 3-Phosphoglycerate (3-PG)=2.3; GMP=17.1; IMP=11.2;CMP=11.5 | 7.2 | 30 | 25 µmol imidazole-HCl, 25 µmol KCl, 3.5 µmol MgCl2, 2 µmol ADP, 0.06 µmol NADH, 01.0 mM activator, 10 µg LDH | R5P>AMP>GMP>CMP>IMP>3-PG>F6P=G6P>none>G1P>FBP | relative activity | ||||||||||||||||||||||||||||||||||||||
378 | 8125251 | Eimeria tenella | Denton_FEMSMicrobLett_vol115_pp87-92_1994_ EimeriaTenella_PYK-Glc | 10 | Km | 5.2 | 0.26 | 7.5 | 41 | 50 mM Hepes, 1mM DTT, 7.5 mM MgCl2, 75 mM KCl, 0.2 mg NADH, 2 U LDH, 5 mM ADP | none>G6P | Km | |||||||||||||||||||||||||||||||||||||||||||||
379 | 8125251 | Eimeria tenella | Denton_FEMSMicrobLett_vol115_pp87-92_1994_ EimeriaTenella_PYK-Glc | 10 | Vmax [nmol/min] | 3 | 3.7 | 7.5 | 41 | 50 mM Hepes, 1mM DTT, 7.5 mM MgCl2, 75 mM KCl, 0.2 mg NADH, 2 U LDH, 5 mM ADP | G6P>none | Vmax | |||||||||||||||||||||||||||||||||||||||||||||
380 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; WT | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 480 | 22 | 450 | 17 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||
381 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant F389Y | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 470 | 10 | 490 | 7 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | FBP>none | Vmax | ||||||||||||||||||||||||||||||||||||||||||
382 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 380 | 4 | 380 | 7 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none=FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||
383 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant S401A/S402P | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 470 | 9 | 450 | 15 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||
384 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L408T | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 540 | 11 | 500 | 21 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||
385 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L423C | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 490 | 26 | 510 | 6 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | FBP>=none | Vmax | ||||||||||||||||||||||||||||||||||||||||||
386 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L427I | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | Vmax/app [µmol/min/mg] | 500 | 10 | 490 | 14 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>=FBP | Vmax | ||||||||||||||||||||||||||||||||||||||||||
387 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; WT | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.049 | 0.01 | 0.045 | 0.006 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
388 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant F389Y | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.11 | 0.006 | 0.061 | 0.003 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
389 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.41 | 0.007 | 0.23 | 0.01 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
390 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant S401A/S402P | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.13 | 0.007 | 0.054 | 0.006 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
391 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L408T | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.089 | 0.006 | 0.038 | 0.006 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
392 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L423C | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.17 | 0.022 | 0.1 | 0.003 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
393 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant L427I | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | S0,5 | 0.068 | 0.004 | 0.049 | 0.005 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | S0,5 | ||||||||||||||||||||||||||||||||||||||||||
394 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; WT | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 1 | 0.15 | 1 | 0.1 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none=FBP | nH | ||||||||||||||||||||||||||||||||||||||||||
395 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant F389Y | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 2.1 | 0.19 | 1.6 | 0.09 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||
396 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant A398R | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 2.7 | 0.1 | 2.2 | 0.18 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | nH | ||||||||||||||||||||||||||||||||||||||||||
397 | 9252361 | rat (expressed in Spodoptera frugiperda) | M1; mutant S401A/S402P | Ikeda_JBC_vol272_pp20495-501_1997_PYK-M1_mutants | 0.01 - 2.0 | nH | 1.7 | 0.12 | 1.4 | 0.18 | 7.5 | 37 | 50 mM Tris-HCl, 0.1 M KCl, 5 mM MgSO4, +/- 1 mM FBP, 2 mM ADP, 0.17 mM NADH, 17 U LDH; 1 mL | none>FBP | nH |
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