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Read.Me
Sheet1
Results-std curve
Repeat for thiamin
Graph-stats
Graph-stats (2)
Chromatogram
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Testing thiamine vitamers in plant samples from study L&H3 | |||||||||
Samples of Col and prr7prr9 plants from day 27 end of night EN - day 28 end of day ED and EN, as analysed previously for standard 12 metabolites | |||||||||
Analysis Protocol | From 'Methods' - the extraction of B1 vitamers was performed using 100 mg of plant tissue homogenised in 200 µL of 1% (v/v) trichloroacetic acid. The mixture was centrifuged for 10 min at 10000 g and the supernatant decanted. B1 vitamers were derivatized and quantified by an HPLC method as described (Moulin et al. 2013). The data were normalized to the tissue fresh weight. | ||||||||
Sheets of this workbook | Contents | ||||||||
Sheet1 | Raw data and sampling plan | ||||||||
Results-std curve | Data for individual replicates and standard curve | ||||||||
Repeat for thiamin | |||||||||
Graph-stats | Summary statistics, means, t-test statistics except thiamine, and final charts | ||||||||
Graph-stats (2) | Summary statistics, means, t-test statistics only for thiamine | ||||||||
Chromatogram | raw data traces | ||||||||
Credit | |||||||||
Experimental design, plant growth and sampling | Yin Hoon Chew, Andrew Millar group University of Edinburgh | ||||||||
Analytical design, execution and data analysis | Michael Moulin, Teresa Fitzpatrick group University of Geneva | ||||||||
File Curation | Andrew Millar | ||||||||
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numbering | Col | prr7,prr9 | replicat | Col | prr7,prr9 | |||||||||||||
27 EN | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 27 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 ED | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 28 ED | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
28 EN | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 28 EN | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
mass tube | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.9438 | 0.9547 | 0.9455 | 0.9373 | 0.9437 | 0.9561 | 0.9486 | 0.9465 | ||||||||||
28 ED | 0.9427 | 0.9391 | 0.9409 | 0.9434 | 0.9522 | 0.9437 | 0.9438 | 0.9442 | ||||||||||
28 EN | 0.944 | 0.9421 | 0.9439 | 0.9465 | 0.9562 | 0.9436 | 0.9506 | 0.9442 | ||||||||||
mass tube+sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 1.0289 | 1.0105 | 0.992 | 0.9987 | 1.0115 | 1.0188 | 1.0039 | 1.0221 | ||||||||||
28 ED | 1.0234 | 1.036 | 1.0222 | 1.0111 | 1.0581 | 0.9944 | 0.9984 | 1.0461 | ||||||||||
28 EN | 1.0562 | 1.0187 | 1.0434 | 1.0493 | 1.0576 | 1.0668 | 1.0598 | 1.0178 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 0.08509999999999995 | 0.05579999999999996 | 0.046499999999999986 | 0.06140000000000001 | 0.06780000000000008 | 0.06269999999999998 | 0.055300000000000016 | 0.0756 | ||||||||||
28 ED | 0.0807000000000001 | 0.09689999999999999 | 0.08130000000000004 | 0.0677000000000001 | 0.1059 | 0.05069999999999997 | 0.05459999999999998 | 0.10189999999999999 | ||||||||||
28 EN | 0.11220000000000008 | 0.07659999999999989 | 0.09950000000000014 | 0.10279999999999989 | 0.10140000000000005 | 0.12319999999999998 | 0.10920000000000007 | 0.0736 | ||||||||||
mass sample | Col | prr7,prr9 | ||||||||||||||||
27 EN | 300 | 200 | 200 | 200 | 200 | 200 | 200 | 300 | ||||||||||
28 ED | 300 | 300 | 300 | 200 | 400 | 200 | 200 | 400 | ||||||||||
28 EN | 300 | 300 | 300 | 300 | 40 | 400 | 400 | 300 | ||||||||||
Extraxtion volume | Mass | Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | 300 | 85.09999999999995 | 0.03125 | pmol | 98.93505859375 | 100.81101989746094 | 217.923583984375 | |||||||||||
2 | 200 | 55.79999999999996 | 0.0625 | pmol | 252.35 | 237.66 | 289.42 | |||||||||||
3 | 200 | 46.499999999999986 | 0.125 | pmol | 309.66 | 318.61 | 576.96 | |||||||||||
4 | 200 | 61.40000000000001 | 0.25 | pmol | 502.94 | 515.39 | 955.81 | |||||||||||
5 | 200 | 67.80000000000008 | 0.5 | pmol | 850.95 | 859.87 | 1269.7 | |||||||||||
6 | 200 | 62.699999999999974 | 1 | pmol | 1621.6 | 1673.7 | 2243.1 | |||||||||||
7 | 200 | 55.30000000000002 | 2 | pmol | 3490.9 | 3645 | 4747 | |||||||||||
8 | 300 | 75.6 | 4 | pmol | 6814.1 | 7077 | 9161 | |||||||||||
9 | 300 | 80.7000000000001 | 1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 300 | 85.09999999999995 | |||||||||
10 | 300 | 96.89999999999999 | 9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 300 | 80.7000000000001 | |||||||||
11 | 300 | 81.30000000000004 | 17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 300 | 112.20000000000007 | |||||||||
12 | 200 | 67.70000000000009 | 5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 200 | 67.80000000000008 | |||||||||
13 | 400 | 105.89999999999999 | 13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 400 | 105.89999999999999 | |||||||||
14 | 200 | 50.69999999999997 | 21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 400 | 101.40000000000005 | |||||||||
15 | 200 | 54.59999999999998 | Standard | 1 | 1645.3131103515625 | 1719.94091796875 | 2256.296630859375 | |||||||||||
16 | 400 | 101.89999999999999 | 2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 200 | 55.79999999999996 | |||||||||
17 | 300 | 112.20000000000007 | 10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 300 | 96.89999999999999 | |||||||||
18 | 200 | 76.5999999999999 | 18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 200 | 76.5999999999999 | |||||||||
19 | 300 | 99.50000000000014 | 6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 200 | 62.699999999999974 | |||||||||
20 | 300 | 102.7999999999999 | 14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 200 | 50.69999999999997 | |||||||||
21 | 400 | 101.40000000000005 | 22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 400 | 123.19999999999997 | |||||||||
22 | 400 | 123.19999999999997 | Standard | 0.5 | 808.2450561523438 | 831.448486328125 | 1122.8255615234375 | |||||||||||
23 | 400 | 109.20000000000007 | 3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 200 | 46.499999999999986 | |||||||||
24 | 300 | 73.6 | 11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 300 | 81.30000000000004 | |||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 300 | 99.50000000000014 | ||||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 200 | 55.30000000000002 | ||||||||||||
Derivatization | 15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 200 | 54.59999999999998 | |||||||||||
25 µL | 23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 400 | 109.20000000000007 | |||||||||||
injection | injection | Standard | 1 | 1606.5908203125 | 1679.7308349609375 | 2153.149169921875 | ||||||||||||
20 µL | 4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 200 | 61.40000000000001 | |||||||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 200 | 67.70000000000009 | ||||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 300 | 102.7999999999999 | ||||||||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 300 | 75.6 | ||||||||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 400 | 101.89999999999999 | ||||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 300 | 73.6 | ||||||||||||
Standard | 0.25 | 447.85986328125 | 458.4064636230469 | 788.3421020507812 | ||||||||||||||
Standard | 0.03125 | 98.93505859375 | 100.81101989746094 | 217.923583984375 |
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Sample analysis order | TDP | TMP | THIA | Extraction volume | Mass | |||||||||||
1 | Col I | 2180.39501953125 | 301.9862060546875 | 83.60588073730469 | 3.0E-4 | 0.08509999999999995 | ||||||||||
2 | Col II | 2157.2509765625 | 176.75811767578125 | 54.220611572265625 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||||||
3 | Col III | 2016.7115478515625 | 173.78077697753906 | 65.31547546386719 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||||||
4 | Col IV | 2237.2646484375 | 125.17689514160156 | 43.60393142700195 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||||||
5 | prr7,9 I | 2514.615478515625 | 299.6667175292969 | 70.3182601928711 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||||||
6 | prr7,9 II | 2416.207275390625 | 603.93408203125 | 58.665550231933594 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||||||
7 | prr7,9 III | 2498.947509765625 | 391.9966735839844 | 105.10193634033203 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | TDP | TMP | Thia | ||||||
8 | prr7,9 IV | 2290.056396484375 | 659.33 | 345.0472412109375 | 3.0E-4 | 0.0756 | 3.125E-14 | 98.93505859375 | 100.81101989746094 | 217.923583984375 | ||||||
9 | Col I | 1917.8831787109375 | 375.14276123046875 | 132.87686157226562 | 3.0E-4 | 0.0807000000000001 | 6.25E-14 | 252.35 | 237.66 | 289.42 | ||||||
10 | Col II | 1865.593994140625 | 616.8743286132812 | 59.84711456298828 | 3.0E-4 | 0.0969 | 1.25E-13 | 309.66 | 318.61 | 576.96 | ||||||
11 | Col III | 1981.958740234375 | 174.57398986816406 | 21.63164520263672 | 3.0E-4 | 0.08130000000000004 | 2.5E-13 | 502.94 | 515.39 | 955.81 | ||||||
12 | Col IV | 2196.937255859375 | 269.585 | 42.28960418701172 | 1.9999999999999998E-4 | 0.0677000000000001 | 5.0E-13 | 850.95 | 859.87 | 1269.7 | ||||||
13 | prr7,9 I | 1365.0078125 | 1249.138671875 | 116.79225158691406 | 3.9999999999999996E-4 | 0.1059 | 1.0E-12 | 1621.6 | 1673.7 | 2243.1 | ||||||
14 | prr7,9 II | 2289.3037109375 | 526.7593383789062 | 127.31964874267578 | 1.9999999999999998E-4 | 0.05069999999999997 | 2.0E-12 | 3490.9 | 3645 | 4747 | ||||||
15 | prr7,9 III | 2427.84375 | 588.3184204101562 | 63.29181671142578 | 1.9999999999999998E-4 | 0.05459999999999998 | 4.0E-12 | 6814.1 | 7077 | 9161 | ||||||
16 | prr7,9 IV | 1518.505859375 | 1229.6 | 95.14126586914062 | 3.9999999999999996E-4 | 0.10189999999999999 | Slope | 1.685774878178111E15 | 1.7555770182597208E15 | 2.2371156417932975E15 | ||||||
17 | Col I | 2178.0859375 | 806.1314697265625 | 72.3534927368164 | 3.0E-4 | 0.11220000000000008 | b | 63.49 | 54.786 | 204.24 | ||||||
18 | Col II | 2377.778564453125 | 465.9914245605469 | 59.2650146484375 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||||||
19 | Col III | 2064.210693359375 | 473.5988464355469 | 64.27493286132812 | 3.0E-4 | 0.09950000000000014 | ||||||||||
20 | Col IV | 2176.550048828125 | 417.441 | 73.04740905761719 | 3.0E-4 | 0.1027999999999999 | ||||||||||
21 | prr7,9 I | 1654.855712890625 | 1042.51416015625 | 116.10993957519531 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||||||
22 | prr7,9 II | 1362.8040771484375 | 1686.056884765625 | 129.24539184570312 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||||||
23 | prr7,9 III | 1696.642333984375 | 1158.29 | 123.79141235351562 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||||||
24 | prr7,9 IV | 2028.2176513671875 | 424.313 | 69.16194915771484 | 3.0E-4 | 0.0736 | ||||||||||
Sample analysis order | pmol injected | TDP | TMP | THIA | Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||
1 | Col I | 1.2557459758915614E-12 | 1.4080852248779928E-13 | 4.180294036865234E-14 | 27 EN | 1 | Col I | 2.511491951783123E-7 | 2.8161704497559855E-8 | 8.360588073730468E-9 | ||||||
2 | Col II | 1.2420169523615854E-12 | 6.947693915285502E-14 | 2.7110305786132812E-14 | 2 | Col II | 2.4840339047231705E-7 | 1.3895387830571003E-8 | 5.422061157226562E-9 | |||||||
3 | Col III | 1.1586491014520826E-12 | 6.778100632434624E-14 | 3.265773773193359E-14 | 3 | Col III | 2.3172982029041653E-7 | 1.3556201264869248E-8 | 6.531547546386718E-9 | |||||||
4 | Col IV | 1.289480983835037E-12 | 4.009558931876374E-14 | 2.1801965713500978E-14 | 4 | Col IV | 2.5789619676700736E-7 | 8.019117863752749E-9 | 4.360393142700195E-9 | |||||||
5 | prr7,9 I | 1.4540052234997483E-12 | 1.3948731099934524E-13 | 3.515913009643555E-14 | 5 | prr7,9 I | 2.908010446999496E-7 | 2.7897462199869047E-8 | 7.0318260192871094E-9 | |||||||
6 | prr7,9 II | 1.3956295741773702E-12 | 3.1280204532160763E-13 | 2.93327751159668E-14 | 6 | prr7,9 II | 2.79125914835474E-7 | 6.256040906432153E-8 | 5.8665550231933595E-9 | |||||||
7 | prr7,9 III | 1.4447109998446106E-12 | 1.9207968096908483E-13 | 5.2550968170166016E-14 | 7 | prr7,9 III | 2.889421999689221E-7 | 3.8415936193816965E-8 | 1.0510193634033203E-8 | |||||||
8 | prr7,9 IV | 1.3207969968627843E-12 | 3.443562963698831E-13 | 1.7252362060546875E-13 | 8 | prr7,9 IV | 2.6415939937255685E-7 | 6.887125927397661E-8 | 3.450472412109375E-8 | |||||||
9 | Col I | 1.1000242100624132E-12 | 1.824794685157328E-13 | 6.643843078613281E-14 | 28 EN | 9 | Col I | 2.2000484201248262E-7 | 3.6495893703146554E-8 | 1.328768615722656E-8 | ||||||
10 | Col II | 1.0690063171947585E-12 | 3.2017298174162225E-13 | 2.992355728149414E-14 | 10 | Col II | 2.138012634389517E-7 | 6.403459634832445E-8 | 5.9847114562988275E-9 | |||||||
11 | Col III | 1.1380337701483285E-12 | 6.823283092809465E-14 | 1.081582260131836E-14 | 11 | Col III | 2.2760675402966568E-7 | 1.364656618561893E-8 | 2.1631645202636718E-9 | |||||||
12 | Col IV | 1.2655588142143173E-12 | 1.2235236492952458E-13 | 2.114480209350586E-14 | 12 | Col IV | 2.5311176284286343E-7 | 2.4470472985904917E-8 | 4.228960418701171E-9 | |||||||
13 | prr7,9 I | 7.72059086505433E-13 | 6.803191540174895E-13 | 5.839612579345703E-14 | 13 | prr7,9 I | 1.5441181730108656E-7 | 1.360638308034979E-7 | 1.1679225158691405E-8 | |||||||
14 | prr7,9 II | 1.3203505045365442E-12 | 2.6884228573849006E-13 | 6.365982437133789E-14 | 14 | prr7,9 II | 2.640701009073088E-7 | 5.376845714769801E-8 | 1.2731964874267577E-8 | |||||||
15 | prr7,9 III | 1.4025323194727273E-12 | 3.039071569409353E-13 | 3.164590835571289E-14 | 15 | prr7,9 III | 2.8050646389454546E-7 | 6.078143138818706E-8 | 6.329181671142578E-9 | |||||||
16 | prr7,9 IV | 8.63113976966781E-13 | 6.691896668621105E-13 | 4.757063293457031E-14 | 16 | prr7,9 IV | 1.726227953933562E-7 | 1.338379333724221E-7 | 9.514126586914061E-9 | |||||||
17 | Col I | 1.254376230701299E-12 | 4.2797636441570696E-13 | 3.6176746368408206E-14 | 28 ED | 17 | Col I | 2.5087524614025976E-7 | 8.559527288314139E-8 | 7.235349273681641E-9 | ||||||
18 | Col II | 1.3728337006387685E-12 | 2.3422807446418336E-13 | 2.963250732421875E-14 | 18 | Col II | 2.745667401277537E-7 | 4.684561489283667E-8 | 5.92650146484375E-9 | |||||||
19 | Col III | 1.1868255478582284E-12 | 2.3856136306153646E-13 | 3.213746643066406E-14 | 19 | Col III | 2.3736510957164565E-7 | 4.771227261230729E-8 | 6.427493286132813E-9 | |||||||
20 | Col IV | 1.2534651430514847E-12 | 2.065731074330734E-13 | 3.652370452880859E-14 | 20 | Col IV | 2.5069302861029693E-7 | 4.1314621486614676E-8 | 7.304740905761718E-9 | |||||||
21 | prr7,9 I | 9.439965759903138E-13 | 5.626230862462367E-13 | 5.805496978759765E-14 | 21 | prr7,9 I | 1.8879931519806277E-7 | 1.1252461724924733E-7 | 1.161099395751953E-8 | |||||||
22 | prr7,9 II | 7.707518328619666E-13 | 9.291935744195838E-13 | 6.462269592285156E-14 | 22 | prr7,9 II | 1.541503665723933E-7 | 1.8583871488391673E-7 | 1.2924539184570312E-8 | |||||||
23 | prr7,9 III | 9.687843585315452E-13 | 6.285705432017378E-13 | 6.189570617675781E-14 | 23 | prr7,9 III | 1.9375687170630903E-7 | 1.2571410864034754E-7 | 1.2379141235351562E-8 | |||||||
24 | prr7,9 IV | 1.16547451074283E-12 | 2.1048748995718058E-13 | 3.458097457885742E-14 | 24 | prr7,9 IV | 2.3309490214856598E-7 | 4.209749799143611E-8 | 6.9161949157714844E-9 | |||||||
Sample analysis order | nmol/gFW | TDP | TMP | THIA | ||||||||||||
1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | Mean | TDP | TMP | THIA | ||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | 27 EN | Col | 903.1105514312983 | 58.377214678324826 | 22.800776756334205 | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 28 ED | Col | 766.8519602323545 | 114.14230855984623 | 22.100128563367562 | |||||||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28 EN | Col | 708.7355014572627 | 153.90018407206077 | 18.87911546319028 | |||||||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 27 EN | prr7,9 | 960.3561214730375 | 173.5208146716876 | 53.59775060445553 | |||||||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | 28 ED | prr7,9 | 832.5114124761428 | 368.51248577692866 | 38.71740225546809 | |||||||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | 28 EN | prr7,9 | 726.2764745957589 | 419.8352113851851 | 40.32534466004294 | |||||||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | ||||||||||||
9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | STDEV | TDP | TMP | THIA | ||||||||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | 27 EN | Col | 33.180008622075476 | 15.239203092020402 | 3.626063867843288 | |||||||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | 28 ED | Col | 40.11419122603355 | 33.36385488473636 | 9.351753009264181 | |||||||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | 28 EN | Col | 13.156048540266871 | 25.54300793249725 | 1.2250361762140936 | |||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | 27 EN | prr7,9 | 50.252659703550506 | 40.97956675158741 | 28.11063756404265 | |||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | 28 ED | prr7,9 | 118.28920963749397 | 87.31786360652637 | 5.807422031023975 | |||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | 28 EN | prr7,9 | 92.05967843469897 | 90.15789119686292 | 4.134412699440083 | |||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | ||||||||||||
17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | Fold vs Col | TDP | TMP | THIA | ||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | 27 EN | Col | ||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | 28 ED | Col | ||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | 28 EN | Col | ||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | 27 EN | prr7,9 | 1.0633871124094536 | 2.9724065395691963 | 2.3506984510764846 | |||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | 28 ED | prr7,9 | 1.0856220700327788 | 3.2285354171167215 | 1.7519084626342294 | |||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | 28 EN | prr7,9 | 1.0247496747410416 | 2.7279708202857345 | 2.135976377636316 | |||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 |
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Sample analysis order | THIA | Extraction volume | Mass | |||||||
1 | Col I | 285.237 | 3.0E-4 | 0.08509999999999995 | ||||||
2 | Col II | 121.45 | 1.9999999999999998E-4 | 0.05579999999999996 | ||||||
3 | Col III | 160.685 | 1.9999999999999998E-4 | 0.046499999999999986 | ||||||
4 | Col IV | 150.074 | 1.9999999999999998E-4 | 0.06140000000000002 | ||||||
5 | prr7,9 I | 272.37 | 1.9999999999999998E-4 | 0.06780000000000008 | ||||||
6 | prr7,9 II | 242.808 | 1.9999999999999998E-4 | 0.06269999999999998 | ||||||
7 | prr7,9 III | 164.77 | 1.9999999999999998E-4 | 0.05530000000000002 | pmol | Thia | ||||
8 | prr7,9 IV | 855.881 | 3.0E-4 | 0.0756 | 6.25E-14 | 291.3258 | ||||
9 | Col I | 203.8 | 3.0E-4 | 0.0807000000000001 | 1.25E-13 | 464.7634 | ||||
10 | Col II | 156.916 | 3.0E-4 | 0.0969 | 2.5E-13 | 711.93347 | ||||
11 | Col III | 135.305 | 3.0E-4 | 0.08130000000000004 | 5.0E-13 | 964.34528 | ||||
12 | Col IV | 162.112 | 1.9999999999999998E-4 | 0.0677000000000001 | 1.0E-12 | 1763.112 | ||||
13 | prr7,9 I | 330.51 | 3.9999999999999996E-4 | 0.1059 | 2.0E-12 | 4222.5888 | ||||
14 | prr7,9 II | 166.392 | 1.9999999999999998E-4 | 0.05069999999999997 | Slope | 1.9906732241935485E15 | ||||
15 | prr7,9 III | 288.228 | 1.9999999999999998E-4 | 0.05459999999999998 | b | 256.55 | ||||
16 | prr7,9 IV | 273.176 | 3.9999999999999996E-4 | 0.10189999999999999 | ||||||
17 | Col I | 324.4 | 3.0E-4 | 0.11220000000000008 | ||||||
18 | Col II | 187.35 | 1.9999999999999998E-4 | 0.07659999999999989 | ||||||
19 | Col III | 164.641 | 3.0E-4 | 0.09950000000000014 | ||||||
20 | Col IV | 206.925 | 3.0E-4 | 0.1027999999999999 | ||||||
21 | prr7,9 I | 307.41 | 3.9999999999999996E-4 | 0.10140000000000005 | ||||||
22 | prr7,9 II | 445.663 | 3.9999999999999996E-4 | 0.12319999999999998 | ||||||
23 | prr7,9 III | 166.888 | 3.9999999999999996E-4 | 0.10920000000000007 | ||||||
24 | prr7,9 IV | 184.335 | 3.0E-4 | 0.0736 | ||||||
Sample analysis order | pmol injected | THIA | Sample analysis order | pmol/µL | THIA | |||||
1 | Col I | 1.4261850000000002E-13 | 27 EN | 1 | Col I | 1.140948E-8 | ||||
2 | Col II | 6.0725E-14 | 2 | Col II | 4.8579999999999996E-9 | |||||
3 | Col III | 8.03425E-14 | 3 | Col III | 6.4274E-9 | |||||
4 | Col IV | 7.503700000000001E-14 | 4 | Col IV | 6.0029600000000005E-9 | |||||
5 | prr7,9 I | 1.36185E-13 | 5 | prr7,9 I | 1.0894799999999998E-8 | |||||
6 | prr7,9 II | 1.21404E-13 | 6 | prr7,9 II | 9.71232E-9 | |||||
7 | prr7,9 III | 8.238500000000001E-14 | 7 | prr7,9 III | 6.5908E-9 | |||||
8 | prr7,9 IV | 4.279405E-13 | 8 | prr7,9 IV | 3.423524E-8 | |||||
9 | Col I | 1.019E-13 | 28 EN | 9 | Col I | 8.151999999999999E-9 | ||||
10 | Col II | 7.8458E-14 | 10 | Col II | 6.276639999999999E-9 | |||||
11 | Col III | 6.76525E-14 | 11 | Col III | 5.4122E-9 | |||||
12 | Col IV | 8.1056E-14 | 12 | Col IV | 6.4844799999999995E-9 | |||||
13 | prr7,9 I | 1.6525499999999999E-13 | 13 | prr7,9 I | 1.3220399999999999E-8 | |||||
14 | prr7,9 II | 8.3196E-14 | 14 | prr7,9 II | 6.65568E-9 | |||||
15 | prr7,9 III | 1.4411400000000002E-13 | 15 | prr7,9 III | 1.152912E-8 | |||||
16 | prr7,9 IV | 1.3658799999999998E-13 | 16 | prr7,9 IV | 1.0927039999999999E-8 | |||||
17 | Col I | 1.6219999999999998E-13 | 28 ED | 17 | Col I | 1.2975999999999998E-8 | ||||
18 | Col II | 9.3675E-14 | 18 | Col II | 7.493999999999999E-9 | |||||
19 | Col III | 8.23205E-14 | 19 | Col III | 6.58564E-9 | |||||
20 | Col IV | 1.034625E-13 | 20 | Col IV | 8.277E-9 | |||||
21 | prr7,9 I | 1.5370500000000003E-13 | 21 | prr7,9 I | 1.2296400000000002E-8 | |||||
22 | prr7,9 II | 2.228315E-13 | 22 | prr7,9 II | 1.782652E-8 | |||||
23 | prr7,9 III | 8.344400000000001E-14 | 23 | prr7,9 III | 6.6755200000000005E-9 | |||||
24 | prr7,9 IV | 9.216750000000001E-14 | 24 | prr7,9 IV | 7.3734E-9 | |||||
Sample analysis order | nmol/gFW | THIA | ||||||||
1 | Col I | 40.22143360752058 | THIA | |||||||
2 | Col II | 17.412186379928322 | 27 EN | Col | 26.207991701355937 | |||||
3 | Col III | 27.644731182795706 | 28 ED | Col | 22.216221609963032 | |||||
4 | Col IV | 19.55361563517915 | 28 EN | Col | 24.568159266491413 | |||||
5 | prr7,9 I | 32.138053097345086 | 27 EN | prr7,9 | 55.70224879793622 | |||||
6 | prr7,9 II | 30.980287081339725 | 28 ED | prr7,9 | 40.32873922161233 | |||||
7 | prr7,9 III | 23.83652802893308 | 28 EN | prr7,9 | 40.22297358543073 | |||||
8 | prr7,9 IV | 135.854126984127 | ||||||||
9 | Col I | 30.3048327137546 | THIA | |||||||
10 | Col II | 19.432321981424142 | 27 EN | Col | 5.16468526370469 | |||||
11 | Col III | 19.971217712177108 | 28 ED | Col | 2.701505439732336 | |||||
12 | Col IV | 19.15651403249628 | 28 EN | Col | 3.534897659186522 | |||||
13 | prr7,9 I | 49.935410764872515 | 27 EN | prr7,9 | 26.780270173884016 | |||||
14 | prr7,9 II | 26.2551479289941 | 28 ED | prr7,9 | 5.004580445280961 | |||||
15 | prr7,9 III | 42.2312087912088 | 28 EN | prr7,9 | 7.812557334225081 | |||||
16 | prr7,9 IV | 42.893189401373895 | ||||||||
17 | Col I | 34.695187165775366 | ||||||||
18 | Col II | 19.566579634464773 | ||||||||
19 | Col III | 19.856201005025095 | ||||||||
20 | Col IV | 24.15466926070041 | ||||||||
21 | prr7,9 I | 48.50650887573963 | ||||||||
22 | prr7,9 II | 57.8783116883117 | ||||||||
23 | prr7,9 III | 24.452454212454196 | ||||||||
24 | prr7,9 IV | 30.054619565217386 |
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Sample analysis order | pmol/µL | TDP | TMP | THIA | |||||||||||||
27 EN | 1 | Col I | 885.3673155522177 | 99.27745416296075 | 29.473283456159127 | ||||||||||||
2 | Col II | 890.3347328756889 | 49.80425745724377 | 19.43391095780131 | TDP | TMP | Thia | TDP | TMP | Thia | |||||||
3 | Col III | 996.6873990985658 | 58.30624199943764 | 28.09267761886761 | 27EN | Col vs prr7.9 | 0.3784950101359991 | 0.03887304751915922 | 0.31894335633099896 | 27EN | Col vs prr7.9 | n.s | * | n.s | |||
4 | Col IV | 840.0527581987208 | 26.12090509365715 | 14.203234992508774 | 28ED | Col vs prr7.9 | 0.6179695046170415 | 0.034584532576066675 | 0.18190213222393758 | 28ED | Col vs prr7.9 | n.s | * | n.s | |||
5 | prr7,9 I | 857.8201908553074 | 82.29339881967259 | 20.742849614416226 | 28EN | Col vs prr7.9 | 0.856606669838744 | 0.029646233508172554 | 0.002517983320163621 | 28EN | Col vs prr7.9 | n.s | * | ** | |||
6 | prr7,9 II | 890.3537953284659 | 199.554733857485 | 18.71309417286558 | Col | 27 EN vs 28 EN | 0.0397230310306063 | 0.17923948406921422 | 0.9465796126527969 | Col | 27 EN vs 28 EN | * | n.s | n.s | |||
7 | prr7,9 III | 1044.9989148966433 | 138.93647809698714 | 38.01155021350162 | prr7.9 | 27 EN vs 28 EN | 0.3582725251207178 | 0.08970590900428527 | 0.6227325689414196 | prr7.9 | 27 EN vs 28 EN | n.s | n.s | n.s | |||
8 | prr7,9 IV | 1048.2515848117334 | 273.2986479126056 | 136.92350841703868 | Col | 28 EN vs 28 ED | 0.21778917569917583 | 0.3805817643529822 | 0.7443616614354294 | Col | 28 EN vs 28 ED | n.s | n.s | n.s | |||
28 EN | 9 | Col I | 817.8618662174064 | 135.6724672979424 | 49.39660281496856 | prr7.9 | 28 EN vs 28 ED | 0.5050727905942308 | 0.6967992204231616 | 0.8290329831982349 | prr7.9 | 28 EN vs 28 ED | n.s | n.s | n.s | ||
10 | Col II | 661.9234162196647 | 198.24952429821812 | 18.528518440553643 | Col | 27 EN vs 28 ED | 0.0015937499093712326 | 0.018331131421305904 | 0.3450625364371603 | Col | 27 EN vs 28 ED | ** | * | n.s | |||
11 | Col III | 839.8773211426773 | 50.356332788261696 | 7.982156901341957 | prr7.9 | 27 EN vs 28 ED | 0.06709678940629217 | 0.047343595565421566 | 0.6568931221269751 | prr7.10 | 27 EN vs 28 ED | n.s | * | n.s | |||
12 | Col IV | 747.7452373496694 | 72.29090985496272 | 12.4932360966061 | |||||||||||||
13 | prr7,9 I | 583.2363259719983 | 513.9332608252989 | 44.114164905349966 | |||||||||||||
14 | prr7,9 II | 1041.6966505219289 | 212.10436744654058 | 50.22471350795891 | |||||||||||||
15 | prr7,9 III | 1027.4962047419249 | 222.64260581753507 | 23.18381564521091 | |||||||||||||
16 | prr7,9 IV | 677.6164686687191 | 525.36970901834 | 37.346914963352546 | |||||||||||||
28 ED | 17 | Col I | 670.7894281825121 | 228.86436599770408 | 19.345853672945548 | ||||||||||||
18 | Col II | 716.8844389758591 | 122.31231042516119 | 15.473894164082918 | |||||||||||||
19 | Col III | 715.6736972009405 | 143.85609832856449 | 19.379376742108956 | |||||||||||||
20 | Col IV | 731.5944414697387 | 120.56796153681334 | 21.31733727362371 | |||||||||||||
21 | prr7,9 I | 744.7704741540934 | 443.88409171300697 | 45.802737505007975 | |||||||||||||
22 | prr7,9 II | 500.4882031571212 | 603.3724509218076 | 41.96278956029323 | |||||||||||||
23 | prr7,9 III | 709.7321307923403 | 460.4912404408331 | 45.34483968993242 | |||||||||||||
24 | prr7,9 IV | 950.1150902794808 | 171.59306246509283 | 28.191011884938113 | |||||||||||||
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Sample analysis order | pmol/µL | THIA | |||||||||
27 EN | 1 | Col I | 100.55358401880146 | ||||||||
2 | Col II | 43.530465949820815 | Thia | Thia | |||||||
3 | Col III | 69.11182795698926 | 27EN | Col vs prr7.9 | 0.3210491931812907 | 27EN | Col vs prr7.9 | n.s | |||
4 | Col IV | 48.884039087947876 | 28ED | Col vs prr7.9 | 0.018960465831258648 | 28ED | Col vs prr7.9 | * | |||
5 | prr7,9 I | 80.34513274336271 | 28EN | Col vs prr7.9 | 0.11769982614260949 | 28EN | Col vs prr7.9 | n.s | |||
6 | prr7,9 II | 77.4507177033493 | Col | 27 EN vs 28 EN | 0.5189961799445153 | Col | 27 EN vs 28 EN | n.s | |||
7 | prr7,9 III | 59.5913200723327 | prr7.9 | 27 EN vs 28 EN | 0.5930067610455964 | prr7.9 | 27 EN vs 28 EN | n.s | |||
8 | prr7,9 IV | 339.6353174603173 | Col | 28 EN vs 28 ED | 0.6160276879003956 | Col | 28 EN vs 28 ED | n.s | |||
28 EN | 9 | Col I | 75.76208178438651 | prr7.9 | 28 EN vs 28 ED | 0.9912742373838004 | prr7.9 | 28 EN vs 28 ED | n.s | ||
10 | Col II | 48.58080495356035 | Col | 27 EN vs 28 ED | 0.802073365028804 | Col | 27 EN vs 28 ED | n.s | |||
11 | Col III | 49.92804428044277 | prr7.9 | 27 EN vs 28 ED | 0.5990358884307454 | prr7.10 | 27 EN vs 28 ED | n.s | |||
12 | Col IV | 47.89128508124068 | |||||||||
13 | prr7,9 I | 124.83852691218128 | |||||||||
14 | prr7,9 II | 65.63786982248523 | |||||||||
15 | prr7,9 III | 105.578021978022 | |||||||||
16 | prr7,9 IV | 107.23297350343472 | |||||||||
28 ED | 17 | Col I | 86.73796791443841 | ||||||||
18 | Col II | 48.91644908616194 | |||||||||
19 | Col III | 49.640502512562726 | |||||||||
20 | Col IV | 60.386673151751026 | |||||||||
21 | prr7,9 I | 121.26627218934907 | |||||||||
22 | prr7,9 II | 144.6957792207792 | |||||||||
23 | prr7,9 III | 61.13113553113548 | |||||||||
24 | prr7,9 IV | 75.13654891304348 | |||||||||
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1.4256091238984 | 29.3053865432739 | 30.0077021121979 | 29.3315827846527 | 29.3905556201935 | 1.4256091238984 | 179.3053865432739 | 55.007702112197904 | 29.3315827846527 | 104.3905556201935 | |
1.43280916997869 | 29.3008208274842 | 30.0045788288117 | 29.379802942276 | 29.4426560401917 | 1.43280916997869 | 179.3008208274842 | 55.0045788288117 | 29.379802942276 | 104.44265604019171 | |
1.44000921605899 | 29.296487569809 | 30.0011456012726 | 29.4428169727326 | 29.5075416564942 | 1.44000921605899 | 179.296487569809 | 55.0011456012726 | 29.4428169727326 | 104.5075416564942 | |
1.44720926213928 | 29.2919099330902 | 29.9976170063019 | 29.5259416103363 | 29.5895099639893 | 1.44720926213928 | 179.2919099330902 | 54.9976170063019 | 29.5259416103363 | 104.5895099639893 | |
1.45440930821957 | 29.2864918708801 | 29.993885755539 | 29.6368777751923 | 29.695338010788 | 1.45440930821957 | 179.2864918708801 | 54.993885755539 | 29.6368777751923 | 104.69533801078799 | |
1.46160935429987 | 29.2799890041352 | 29.9900770187378 | 29.7859489917755 | 29.8349320888519 | 1.46160935429987 | 179.2799890041352 | 54.9900770187378 | 29.7859489917755 | 104.8349320888519 | |
1.46880940038016 | 29.2727112770081 | 29.9869239330292 | 29.986321926117 | 30.0216674804688 | 1.46880940038016 | 179.2727112770081 | 54.9869239330292 | 29.986321926117 | 105.0216674804688 | |
1.47600944646046 | 29.2656660079956 | 29.9856543540955 | 30.2542924880982 | 30.2725851535797 | 1.47600944646046 | 179.2656660079956 | 54.9856543540955 | 30.2542924880982 | 105.2725851535797 | |
1.48320949254075 | 29.2601764202118 | 29.9875557422638 | 30.6096494197846 | 30.6083202362061 | 1.48320949254075 | 179.2601764202118 | 54.9875557422638 | 30.6096494197846 | 105.6083202362061 | |
1.49040953862105 | 29.2575001716614 | 29.9935936927796 | 31.0754239559174 | 31.0524940490723 | 1.49040953862105 | 179.2575001716614 | 54.9935936927796 | 31.0754239559174 | 106.0524940490723 | |
1.49760958470134 | 29.2586505413056 | 30.0043523311615 | 31.6769778728485 | 31.6306471824646 | 1.49760958470134 | 179.2586505413056 | 55.0043523311615 | 31.6769778728485 | 106.6306471824646 | |
1.50480963078164 | 29.2645990848541 | 30.0201177597046 | 32.4406981468201 | 32.3686838150025 | 1.50480963078164 | 179.2645990848541 | 55.020117759704604 | 32.4406981468201 | 107.3686838150025 | |
1.51200967686193 | 29.2760968208313 | 30.0409257411957 | 33.392870426178 | 33.2908809185028 | 1.51200967686193 | 179.2760968208313 | 55.0409257411957 | 33.392870426178 | 108.29088091850281 | |
1.51920972294223 | 29.2932689189911 | 30.0664365291596 | 34.5584690570832 | 34.417587518692 | 1.51920972294223 | 179.2932689189911 | 55.0664365291596 | 34.5584690570832 | 109.417587518692 | |
1.52640976902252 | 29.3151557445526 | 30.0956547260285 | 35.9590947628021 | 35.762757062912 | 1.52640976902252 | 179.3151557445526 | 55.0956547260285 | 35.9590947628021 | 110.762757062912 | |
1.53360981510282 | 29.3396711349488 | 30.1271080970764 | 37.6096606254578 | 37.3314619064331 | 1.53360981510282 | 179.3396711349488 | 55.1271080970764 | 37.6096606254578 | 112.3314619064331 | |
1.54080986118311 | 29.3642103672028 | 30.1589965820313 | 39.5140647888184 | 39.1177117824555 | 1.54080986118311 | 179.36421036720282 | 55.1589965820313 | 39.5140647888184 | 114.1177117824555 | |
1.54800990726341 | 29.3863117694855 | 30.1893830299378 | 41.661149263382 | 41.1027550697327 | 1.54800990726341 | 179.3863117694855 | 55.1893830299378 | 41.661149263382 | 116.1027550697327 | |
1.5552099533437 | 29.4035077095032 | 30.216121673584 | 44.0223515033722 | 43.2542443275452 | 1.5552099533437 | 179.4035077095032 | 55.216121673584 | 44.0223515033722 | 118.2542443275452 | |
1.562409999424 | 29.4128894805908 | 30.2365362644196 | 46.5518832206726 | 45.5268561840058 | 1.562409999424 | 179.4128894805908 | 55.2365362644196 | 46.5518832206726 | 120.5268561840058 | |
1.56961004550429 | 29.410856962204 | 30.2471995353699 | 49.189305305481 | 47.8644967079163 | 1.56961004550429 | 179.410856962204 | 55.2471995353699 | 49.189305305481 | 122.8644967079163 | |
1.57681009158459 | 29.3936252593994 | 30.2441775798798 | 51.8638968467713 | 50.2040505409241 | 1.57681009158459 | 179.3936252593994 | 55.2441775798798 | 51.8638968467713 | 125.2040505409241 | |
1.58401013766488 | 29.3582677841187 | 30.2236795425415 | 54.500412940979 | 52.4799525737763 | 1.58401013766488 | 179.3582677841187 | 55.223679542541504 | 54.500412940979 | 127.4799525737763 | |
1.59121018374518 | 29.3034493923187 | 30.1831245422363 | 57.0253312587738 | 54.628849029541 | 1.59121018374518 | 179.3034493923187 | 55.1831245422363 | 57.0253312587738 | 129.62884902954102 | |
1.59841022982547 | 29.2295396327973 | 30.1222145557404 | 59.373414516449 | 56.5940260887146 | 1.59841022982547 | 179.2295396327973 | 55.1222145557404 | 59.373414516449 | 131.5940260887146 | |
1.60561027590577 | 29.1387915611267 | 30.0435721874237 | 61.4934384822846 | 58.3295226097107 | 1.60561027590577 | 179.1387915611267 | 55.0435721874237 | 61.4934384822846 | 133.3295226097107 | |
1.61281032198606 | 29.0355384349823 | 29.9527585506439 | 63.3519113063813 | 59.8037719726563 | 1.61281032198606 | 179.0355384349823 | 54.9527585506439 | 63.3519113063813 | 134.8037719726563 | |
1.62001036806636 | 28.9264619350434 | 29.8576295375824 | 64.9339377880097 | 61.0017538070679 | 1.62001036806636 | 178.9264619350434 | 54.8576295375824 | 64.9339377880097 | 136.0017538070679 | |
1.62721041414665 | 28.8200974464417 | 29.7671914100647 | 66.2411332130432 | 61.9249939918518 | 1.62721041414665 | 178.8200974464417 | 54.7671914100647 | 66.2411332130432 | 136.9249939918518 | |
1.63441046022695 | 28.7256121635437 | 29.6902775764466 | 67.2876834869385 | 62.5891208648682 | 1.63441046022695 | 178.7256121635437 | 54.690277576446604 | 67.2876834869385 | 137.5891208648682 | |
1.64161050630724 | 28.651225566864 | 29.6341717243195 | 68.0966973304749 | 63.0201578140259 | 1.64161050630724 | 178.651225566864 | 54.6341717243195 | 68.0966973304749 | 138.0201578140259 | |
1.64881055238754 | 28.6029815673828 | 29.6034395694733 | 68.6987221240998 | 63.2511079311371 | 1.64881055238754 | 178.6029815673828 | 54.603439569473295 | 68.6987221240998 | 138.25110793113708 | |
1.65601059846783 | 28.5840272903443 | 29.5992255210877 | 69.1320717334748 | 63.3199810981751 | 1.65601059846783 | 178.5840272903443 | 54.5992255210877 | 69.1320717334748 | 138.3199810981751 | |
1.66321064454813 | 28.5944998264313 | 29.6191215515137 | 69.4435775279999 | 63.2687985897065 | 1.66321064454813 | 178.5944998264313 | 54.6191215515137 | 69.4435775279999 | 138.2687985897065 | |
1.67041069062842 | 28.6317765712738 | 29.6579539775849 | 69.6872055530548 | 63.1424248218537 | 1.67041069062842 | 178.6317765712738 | 54.657953977584896 | 69.6872055530548 | 138.1424248218537 | |
1.67761073670872 | 28.6910831928253 | 29.7092616558075 | 69.9195563793183 | 62.9859447479248 | 1.67761073670872 | 178.6910831928253 | 54.709261655807495 | 69.9195563793183 | 137.9859447479248 | |
1.68481078278901 | 28.7664592266083 | 29.7669231891632 | 70.1936781406403 | 62.8406465053559 | 1.68481078278901 | 178.7664592266083 | 54.7669231891632 | 70.1936781406403 | 137.8406465053559 | |
1.69201082886931 | 28.8516044616699 | 29.8262119293213 | 70.551860332489 | 62.7394795417786 | 1.69201082886931 | 178.8516044616699 | 54.8262119293213 | 70.551860332489 | 137.7394795417786 | |
1.6992108749496 | 28.9407134056092 | 29.8841834068299 | 71.0175752639771 | 62.7024948596955 | 1.6992108749496 | 178.9407134056092 | 54.884183406829905 | 71.0175752639771 | 137.7024948596955 | |
1.7064109210299 | 29.0290057659149 | 29.9392342567444 | 71.586811542511 | 62.7325236797333 | 1.7064109210299 | 179.0290057659149 | 54.9392342567444 | 71.586811542511 | 137.7325236797333 | |
1.71361096711019 | 29.1129589080811 | 29.9905478954315 | 72.2208678722382 | 62.8117203712464 | 1.71361096711019 | 179.1129589080811 | 54.990547895431504 | 72.2208678722382 | 137.8117203712464 | |
1.72081101319049 | 29.1901588439942 | 30.0378024578095 | 72.845071554184 | 62.900197505951 | 1.72081101319049 | 179.1901588439942 | 55.037802457809505 | 72.845071554184 | 137.90019750595098 | |
1.72801105927078 | 29.2590320110321 | 30.0810277462006 | 73.3572065830231 | 62.9390776157379 | 1.72801105927078 | 179.2590320110321 | 55.081027746200604 | 73.3572065830231 | 137.93907761573792 | |
1.73521110535108 | 29.3185234069824 | 30.1203906536103 | 73.6445605754853 | 62.8589510917664 | 1.73521110535108 | 179.3185234069824 | 55.1203906536103 | 73.6445605754853 | 137.85895109176641 | |
1.74241115143137 | 29.3679356575012 | 30.156022310257 | 73.6042737960816 | 62.5924408435822 | 1.74241115143137 | 179.3679356575012 | 55.156022310257 | 73.6042737960816 | 137.5924408435822 | |
1.74961119751167 | 29.4069528579712 | 30.1877558231354 | 73.1612801551819 | 62.0874762535096 | 1.74961119751167 | 179.4069528579712 | 55.187755823135404 | 73.1612801551819 | 137.0874762535096 | |
1.75681124359196 | 29.4356405735016 | 30.2152395248413 | 72.2802639007569 | 61.3174259662628 | 1.75681124359196 | 179.4356405735016 | 55.2152395248413 | 72.2802639007569 | 136.31742596626282 | |
1.76401128967226 | 29.4545412063599 | 30.2385032176972 | 70.9708154201508 | 60.2858603000641 | 1.76401128967226 | 179.4545412063599 | 55.2385032176972 | 70.9708154201508 | 135.2858603000641 | |
1.77121133575255 | 29.4647574424744 | 30.2581548690796 | 69.2855000495911 | 59.0253233909607 | 1.77121133575255 | 179.4647574424744 | 55.258154869079604 | 69.2855000495911 | 134.0253233909607 | |
1.77841138183285 | 29.4679939746857 | 30.2752435207367 | 67.3102259635926 | 57.5910925865174 | 1.77841138183285 | 179.4679939746857 | 55.2752435207367 | 67.3102259635926 | 132.5910925865174 | |
1.78561142791314 | 29.4663548469544 | 30.2906632423401 | 65.1480853557587 | 56.0515463352204 | 1.78561142791314 | 179.4663548469544 | 55.2906632423401 | 65.1480853557587 | 131.0515463352204 | |
1.79281147399344 | 29.4620454311371 | 30.3046822547913 | 62.900859117508 | 54.4766783714295 | 1.79281147399344 | 179.46204543113708 | 55.3046822547913 | 62.900859117508 | 129.4766783714295 | |
1.80001152007373 | 29.4570982456207 | 30.3169190883637 | 60.6540501117707 | 52.9273509979248 | 1.80001152007373 | 179.4570982456207 | 55.316919088363704 | 60.6540501117707 | 127.9273509979248 | |
1.80721156615403 | 29.4533550739289 | 30.326920747757 | 58.4690868854523 | 51.4477193355561 | 1.80721156615403 | 179.4533550739289 | 55.326920747757 | 58.4690868854523 | 126.4477193355561 | |
1.81441161223432 | 29.4524312019348 | 30.3345620632172 | 56.3833236694336 | 50.0627219676972 | 1.81441161223432 | 179.45243120193481 | 55.3345620632172 | 56.3833236694336 | 125.0627219676972 | |
1.82161165831462 | 29.4555008411408 | 30.340188741684 | 54.4154584407807 | 48.7809538841248 | 1.82161165831462 | 179.4555008411408 | 55.340188741684 | 54.4154584407807 | 123.7809538841248 | |
1.82881170439491 | 29.462993144989 | 30.3446710109711 | 52.5732636451721 | 47.6008653640747 | 1.82881170439491 | 179.462993144989 | 55.3446710109711 | 52.5732636451721 | 122.6008653640747 | |
1.83601175047521 | 29.4745147228241 | 30.3492903709412 | 50.8612871170044 | 46.5173423290253 | 1.83601175047521 | 179.4745147228241 | 55.349290370941205 | 50.8612871170044 | 121.5173423290253 | |
1.8432117965555 | 29.4891834259033 | 30.355441570282 | 49.2864191532135 | 45.5264508724213 | 1.8432117965555 | 179.4891834259033 | 55.355441570282 | 49.2864191532135 | 120.5264508724213 | |
1.8504118426358 | 29.5060276985169 | 30.3643643856049 | 47.8596687316895 | 44.6272969245911 | 1.8504118426358 | 179.5060276985169 | 55.3643643856049 | 47.8596687316895 | 119.62729692459109 | |
1.85761188871609 | 29.5241713523865 | 30.3766429424286 | 46.5941429138184 | 43.821781873703 | 1.85761188871609 | 179.5241713523865 | 55.3766429424286 | 46.5941429138184 | 118.821781873703 | |
1.86481193479638 | 29.5427024364472 | 30.3920447826386 | 45.5004572868347 | 43.1129813194275 | 1.86481193479638 | 179.5427024364472 | 55.3920447826386 | 45.5004572868347 | 118.11298131942749 | |
1.87201198087668 | 29.5606851577759 | 30.4096698760987 | 44.5822358131409 | 42.5030827522278 | 1.87201198087668 | 179.5606851577759 | 55.409669876098704 | 44.5822358131409 | 117.5030827522278 | |
1.87921202695697 | 29.5774102210999 | 30.4281055927277 | 43.8337802886963 | 41.9915616512299 | 1.87921202695697 | 179.5774102210999 | 55.428105592727704 | 43.8337802886963 | 116.9915616512299 | |
1.88641207303727 | 29.59263920784 | 30.4456233978272 | 43.2407975196839 | 41.5742874145508 | 1.88641207303727 | 179.59263920784 | 55.445623397827205 | 43.2407975196839 | 116.57428741455081 | |
1.89361211911756 | 29.6065092086792 | 30.4605126380921 | 42.7833437919617 | 41.244113445282 | 1.89361211911756 | 179.6065092086792 | 55.4605126380921 | 42.7833437919617 | 116.244113445282 | |
1.90081216519786 | 29.6192228794098 | 30.4717302322388 | 42.4397587776184 | 40.9924626350403 | 1.90081216519786 | 179.6192228794098 | 55.4717302322388 | 42.4397587776184 | 115.9924626350403 | |
1.90801221127815 | 29.6308338642121 | 30.4794728755951 | 42.1903669834137 | 40.8110857009888 | 1.90801221127815 | 179.6308338642121 | 55.4794728755951 | 42.1903669834137 | 115.8110857009888 | |
1.91521225735845 | 29.6413958072663 | 30.4852247238159 | 42.0199453830719 | 40.6929612159729 | 1.91521225735845 | 179.6413958072663 | 55.485224723815904 | 42.0199453830719 | 115.6929612159729 | |
1.92241230343874 | 29.6511828899384 | 30.4909586906433 | 41.9185340404511 | 40.6322896480561 | 1.92241230343874 | 179.6511828899384 | 55.490958690643296 | 41.9185340404511 | 115.6322896480561 | |
1.92961234951904 | 29.6607553958893 | 30.4981648921967 | 41.8807208538056 | 40.6241834163666 | 1.92961234951904 | 179.6607553958893 | 55.4981648921967 | 41.8807208538056 | 115.6241834163666 | |
1.93681239559933 | 29.6707808971405 | 30.5074334144592 | 41.904217004776 | 40.6647264957428 | 1.93681239559933 | 179.6707808971405 | 55.5074334144592 | 41.904217004776 | 115.6647264957428 | |
1.94401244167963 | 29.6818852424622 | 30.5187582969666 | 41.989004611969 | 40.7518446445465 | 1.94401244167963 | 179.68188524246222 | 55.518758296966595 | 41.989004611969 | 115.75184464454651 | |
1.95121248775992 | 29.694664478302 | 30.5319786071778 | 42.1378612518311 | 40.8871412277222 | 1.95121248775992 | 179.694664478302 | 55.5319786071778 | 42.1378612518311 | 115.8871412277222 | |
1.95841253384022 | 29.7096371650696 | 30.5468380451203 | 42.3577427864075 | 41.0781443119049 | 1.95841253384022 | 179.7096371650696 | 55.546838045120296 | 42.3577427864075 | 116.07814431190491 | |
1.96561257992051 | 29.7270834445954 | 30.5628299713135 | 42.6612436771393 | 41.340571641922 | 1.96561257992051 | 179.7270834445954 | 55.562829971313505 | 42.6612436771393 | 116.340571641922 | |
1.97281262600081 | 29.7468662261963 | 30.5793166160584 | 43.0686891078949 | 41.7008399963379 | 1.97281262600081 | 179.7468662261963 | 55.5793166160584 | 43.0686891078949 | 116.70083999633789 | |
1.9800126720811 | 29.7683894634247 | 30.5960893630982 | 43.6116755008698 | 42.1990871429444 | 1.9800126720811 | 179.7683894634247 | 55.5960893630982 | 43.6116755008698 | 117.19908714294439 | |
1.9872127181614 | 29.7909677028656 | 30.6138277053833 | 44.3360269069672 | 42.8902506828308 | 1.9872127181614 | 179.7909677028656 | 55.6138277053833 | 44.3360269069672 | 117.89025068283081 | |
1.99441276424169 | 29.8142433166504 | 30.6341230869293 | 45.2983498573303 | 43.8382089138031 | 1.99441276424169 | 179.8142433166504 | 55.6341230869293 | 45.2983498573303 | 118.8382089138031 | |
2.00161281032199 | 29.8383891582489 | 30.6587398052216 | 46.551913022995 | 45.1008677482605 | 2.00161281032199 | 179.8383891582489 | 55.6587398052216 | 46.551913022995 | 120.1008677482605 | |
2.00881285640228 | 29.86398935318 | 30.6887745857239 | 48.1255114078522 | 46.7102885246277 | 2.00881285640228 | 179.86398935318 | 55.6887745857239 | 48.1255114078522 | 121.7102885246277 | |
2.01601290248258 | 29.8918128013611 | 30.72429895401 | 50.0071346759796 | 48.658150434494 | 2.01601290248258 | 179.8918128013611 | 55.724298954009996 | 50.0071346759796 | 123.65815043449399 | |
2.02321294856287 | 29.9226045608521 | 30.7644248008728 | 52.1420598030091 | 50.8943498134613 | 2.02321294856287 | 179.9226045608521 | 55.7644248008728 | 52.1420598030091 | 125.8943498134613 | |
2.03041299464317 | 29.9568176269531 | 30.8076500892639 | 54.4424116611481 | 53.3344030380249 | 2.03041299464317 | 179.9568176269531 | 55.8076500892639 | 54.4424116611481 | 128.3344030380249 | |
2.03761304072346 | 29.9943506717682 | 30.852210521698 | 56.7976534366608 | 55.8670699596405 | 2.03761304072346 | 179.9943506717682 | 55.852210521698 | 56.7976534366608 | 130.8670699596405 | |
2.04481308680376 | 30.0341427326203 | 30.8962643146515 | 59.0818047523499 | 58.3607435226441 | 2.04481308680376 | 180.0341427326203 | 55.8962643146515 | 59.0818047523499 | 133.3607435226441 | |
2.05201313288405 | 30.074006319046 | 30.9380650520325 | 61.1661612987519 | 60.6768190860749 | 2.05201313288405 | 180.074006319046 | 55.9380650520325 | 61.1661612987519 | 135.6768190860749 | |
2.05921317896435 | 30.1109194755554 | 30.9763073921204 | 62.9453659057617 | 62.696522474289 | 2.05921317896435 | 180.1109194755554 | 55.976307392120404 | 62.9453659057617 | 137.696522474289 | |
2.06641322504464 | 30.1415801048279 | 31.0103416442871 | 64.3682718276978 | 64.3504679203034 | 2.06641322504464 | 180.1415801048279 | 56.010341644287095 | 64.3682718276978 | 139.3504679203034 | |
2.07361327112494 | 30.1630318164826 | 31.0401618480683 | 65.4487848281861 | 65.627783536911 | 2.07361327112494 | 180.1630318164826 | 56.0401618480683 | 65.4487848281861 | 140.627783536911 | |
2.08081331720523 | 30.1732242107392 | 31.0660004615784 | 66.2431180477143 | 66.5548920631409 | 2.08081331720523 | 180.1732242107392 | 56.0660004615784 | 66.2431180477143 | 141.5548920631409 | |
2.08801336328553 | 30.1712274551392 | 31.0877621173859 | 66.8082773685456 | 67.1609103679657 | 2.08801336328553 | 180.1712274551392 | 56.0877621173859 | 66.8082773685456 | 142.1609103679657 | |
2.09521340936582 | 30.157482624054 | 31.1046957969666 | 67.172384262085 | 67.4562573432923 | 2.09521340936582 | 180.157482624054 | 56.104695796966595 | 67.172384262085 | 142.4562573432923 | |
2.10241345544612 | 30.1336228847504 | 31.1154782772064 | 67.3315882682801 | 67.4343168735504 | 2.10241345544612 | 180.1336228847504 | 56.1154782772064 | 67.3315882682801 | 142.43431687355042 | |
2.10961350152641 | 30.1020681858063 | 31.1185717582703 | 67.2603905200959 | 67.0809447765351 | 2.10961350152641 | 180.1020681858063 | 56.1185717582703 | 67.2603905200959 | 142.0809447765351 | |
2.11681354760671 | 30.0654351711273 | 31.1125755310059 | 66.9148623943329 | 66.3740873336792 | 2.11681354760671 | 180.0654351711273 | 56.1125755310059 | 66.9148623943329 | 141.3740873336792 | |
2.124013593687 | 30.0261676311493 | 31.0964286327362 | 66.2294805049897 | 65.2781367301941 | 2.124013593687 | 180.0261676311493 | 56.0964286327362 | 66.2294805049897 | 140.2781367301941 | |
2.1312136397673 | 29.9861907958985 | 31.0696482658386 | 65.1272535324097 | 63.7531340122223 | 2.1312136397673 | 179.9861907958985 | 56.0696482658386 | 65.1272535324097 | 138.7531340122223 | |
2.13841368584759 | 29.9468815326691 | 31.0327291488648 | 63.552987575531 | 61.7847383022309 | 2.13841368584759 | 179.9468815326691 | 56.0327291488648 | 63.552987575531 | 136.7847383022309 |