Disciplines: Not specified
Roles: Not specified
University Education: 1987-1993, Biotechnology (Diploma), Technische Universität Braunschweig, Germany.
Dissertation: 1993-1996, Disseration German Research Centre for Biotechnology, Biochemical Engineering Division, Braunschweig, Germany.
Habiliation: 2006, Saarland University, Saarbrücken, Germany.
1993-1996: Research Assistant, German Research Centre for Biotechnology, Biochemical Engineering Division, Braunschweig, Germany.
1997-1998: Post-doc at Department of Applied Chemistry &
Expertise: yeast, fungi, Metabolomics, Proteomics, Stoichiometric modelling, carbon metabolism, Systems Biology, dynamics and control of biological networks, coupling metabolome and environome, rapid sampling experiments, Biochemistry
Tools: parameter estimation, ODE, HPLC, GC and LC/MS analysis of metabolites, Mass spectrometry (LC-MS/MS), continuous cultivation, Enzyme assay, Material balance based modeling, stimulus response experiments, Chromatography, Fermentation, Matlab, Metabolomics, Biochemistry and protein analysis
I've become a SysMO DB PAL for MOSES project in 2007 being a post-doc in lab of Prof. Matthias Reuss at University of Stuttgart. In the MOSES project, our major efforts were in the experimental data acquisition for dynamic model of primary carbon and anaerobic energy metabolism in yeast. The model implements prediction of perturbations of two types: glucose pulse and temperature jump. We implement “stimulus-response” methodology for the unraveling the dynamic structure of the network and to
experimentally measured extracellular fluxes in yeast Saccharomyces cerevisiae in anaerobic glucose limited chemostat (D=0.1 h-1) on minimal medium
Contributor: Maksim Zakhartsev
Assay type: Metabolite Profiling
Technology type: HPLC
Snapshots: No snapshots
Investigation: Steady state metabolic fluxes and metabolite co...
Organisms: Saccharomyces cerevisiae (chemostat)
Data files: Measured steady state metabolic fluxes
Steady state concentrations of intracellular metabolites in yeast Saccharomyces cerevisiae anaerobic chemostat at D = 0.1 h-1 on minimal medium. All metabolite concentrations are in mmol/L(CV).
Date Published: 1st Apr 2015
Citation: Metabolomics 11(2) : 286
Date Published: 1st Aug 2015
Journal: Journal of Thermal Biology
Citation: Journal of Thermal Biology 52 : 117
Authors: Maksim Zakhartsev, Bock Christian
Date Published: 1st Feb 2010
Journal: Analytical Biochemistry
Date Published: 1st Dec 2011
Journal: Journal of Chromatography B
SAMPLE TYPE to describe samples of CEN.PK-113-7D yeast strain upon metabolic perturbation of the steady state culture by glucose pulse
SEEK ID (Integer) *, STRAIN (SEEK Strain) , Genotype (String) , Cell culture ID (String) , Cell culture start date (Date) , Glucose pulse (concentration) (Integer) , Sampling date (Date) , Time after glucose pulse (Integer) , Cell part (Controlled Vocabulary) , Age at sampling (Integer) *