Determination of essential amino acids for Streptococcus pyogenes
Determination of essential amino acids for Streptococcus pyogenes M49
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Created: 19th May 2011 at 15:32
Last updated: 12th May 2016 at 17:55
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Projects: SysMO-LAB
Institutions: University of Rostock
Comparative Systems Biology: Lactic Acid Bacteria
Programme: SysMO
Public web page: http://www.sysmo.net/index.php?index=57
Basically extending SYSMO-LAB 1st phase into second with addition of fourth species, Lb. plantarum. The main focus is amino acid metabolism. primary metabolisms, like glycolysis is also interest.
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Determination of essential amino acids for Streptococcus pyogenes
Contributor: Araz Zeyniyev
Assay type: Experimental Assay Type
Technology type: Technology Type
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In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose.
Contributor: Araz Zeyniyev
Assay type: Metabolite Profiling
Technology type: Inductively Coupled Plasma Mass Spectrometry
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amino acid auxotrophy for streptococcus pyogenes in CDM-IMIKRO (not CDM-LAB) as function of final growth yield (OD600 at stationary phase) in 96well plates (200 ul culture)
Creator: Araz Zeyniyev
Contributor: Araz Zeyniyev
