SOP for measurement of HK activity in extracts.
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Created: 15th Aug 2014 at 14:43
Last updated: 3rd Mar 2015 at 10:40
Last used: 24th Aug 2019 at 10:26
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys, SulfoSys - Biotec, Whole body modelling of glucose metabolism in malaria patients, FAIRDOM, Molecular Systems Biology, COMBINE Multicellular Modelling, HOTSOLUTE, Steroid biosynthesis, Yeast glycolytic oscillations, Computational pathway design for biotechnological applications
Hypoglycaemia and lactic acidosis are key diagnostics for poor chances of survival in malaria patients. In this project we aim to test to what extent the metabolic activity of Plasmodium falciparum contributes to a changed glucose metabolism in malaria patients. The approach is to start with detailed bottom up models for the parasite and then merge these with more coarse grained models at the whole body level.
Public web page: Not specified
The investigation entails the construction and validation of a detailed mathematical model for glycolysis of the malaria parasite Plasmodium falciparum in the blood stage trophozoite form.
Assays: ALD, ATPASE, Culturing and synchronisation of P. falciparum, ENO, G3PDH, GAPDH, GLC incubation, GLCtr, GLYtr, HK, Inhibition of glucose transport, Inhibition of lactate flux, LACtr, LDH, PFK, PGI, PGK, PGM, PK, PYRtr, Steady state, Supply-demand analysis, TPI, Trophozoite Isolation and Lysate Preparation
This study includes all the experimental data, SOPs and modelling files for the individual reactions used for the model construction.
Person responsible: Jacky Snoep
Snapshots: No snapshots
Kinetic characterisation en mathematical modelling of HK.
Contributor: Dawie Van Niekerk
Biological problem addressed: Model Analysis Type
Snapshots: Snapshot 1
Investigation: Glucose metabolism in Plasmodium falciparum tro...
Study: Model construction
Organisms: No organisms
Models: HK Kinetic model
Data files: HK Kinetic data