Determination of protein phosphorylation levels using Phos-tag™ gels
Version 1

The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.

SEEK ID: https://fairdomhub.org/sops/185?version=1

Filename: Phos-tag conditions v2.pdf 

Format: PDF document

Size: 24.5 KB

Help
help Contributor and Creators
Activity

Views: 1803   Downloads: 6

Created: 18th Jan 2013 at 13:51

Last used: 23rd Feb 2019 at 14:09

help Attributions

None

Related items

Powered by
Seek new full
(v.1.8.0)
Copyright © 2008 - 2019 The University of Manchester and HITS gGmbH