Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with </=1%>3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.
FAIRDOMHub ID: https://seek.sysmo-db.org/publications/265
PubMed ID: 23955772
Journal: Nat Methods
Citation: Nat Methods. 2013 Oct;10(10):996-8. doi: 10.1038/nmeth.2604. Epub 2013 Aug 18.
Date Published: 18th Aug 2013
Author: R. C. Edgar
Created: 8th Jul 2016 at 09:45