SOPs

What is a SOP?

15 SOPs visible to you, out of a total of 17

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DCW measurement

SUMO

No description specified

Creator: Martijn Bekker

Submitter: The JERM Harvester

Investigations: Steady state studies for different oxygen avail...

Studies: Determination of the impact of specific enzyme ..., The Escherichia coli steady state response to o...

Assays: Analysis of by-product formation rates in MG1655, Determination of intracellular metabolite conce..., Determination of intracellular redox state by m..., FNR activity at different aerobiosis levels (st..., Physiological measurements from Sheffield chemo...

Created: 16th Mar 2010 at 17:12

Determination of protein phosphorylation levels using Phos-tag™ gels

SUMO

The phosphorylation level of a particular protein can be determined using a procedure based upon western immunoblotting, with Phos-tag™ reagent present in the SDS-PAGE gel. The Phos-tag™ reagent, supplied in the form of Phos-tag™ acrylamide (Wako Pure Chemical Industries, AAL-107), causes proteins to be resolved both on the basis of size and phosphorylation state. This means that phosphorylated and de-phosphorylated forms of the same protein can be distinguished.

Creator: Matthew Rolfe

Submitter: Matthew Rolfe

Investigations: Steady state studies for different oxygen avail...

Studies: The Escherichia coli steady state response to o...

Assays: ArcA phosphorylation at different aerobiosis le...

Created: 18th Jan 2013 at 12:51

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