Assays

BPG degradation at 70C

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

BPG stability analysis

Contributor: Jacky Snoep

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Samples obtained form the central fermentation facility of Sulfosys have been compared using iTRAQ (isobaric tag for relative and absolute quantification). A pilot experiment resulted in creation of SOP and initial data on cells grown at 70 and 80C

The pilot experiment was conducted in order to create SOPs and to gain insight in transcriptome of cells grown at 70 and 80C

Contributor: Pawel Sierocinski

Assay type: Transcriptional Profiling

Technology type: Microarray

Snapshots: No snapshots

Based on initial cell material, series of SOPs connected with assays of enzymes involved in Central Carbon Metabolism of S. solfataricus have been developed.

Contributor: Pawel Sierocinski

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Experimental data for the conversion of 3PG to F6P and the gluconeogenic pathway intermediates

Kinetic characterisation of fructose 1,6-bisphosphate aldolase phosphatase

Mathematical model for FBPAase kinetics, saturation with DHAP and GAP

Contributor: Jacky Snoep

Biological problem addressed: Enzymology

Snapshots: No snapshots

In order to get uniform data from all geographically separated partners in a consortium, central fermentation facility has been set-up. Based on empirical data, standard procedures for growing S. solfataricus cells have been developed.

Contributor: Pawel Sierocinski

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

Mathematical model for GAPDH kinetics, saturation with BPG, NADPH, NADP, GAP and Pi

Contributor: Jacky Snoep

Biological problem addressed: Enzymology

Snapshots: No snapshots

Pilot experiment concerning metabolome of S. solfataricus was conducted in order to acquire SOPs regarding the technique and gain insight on differences in metabolite concentrations at 70 and 80C

Contributor: Pawel Sierocinski

Assay type: Metabolomics

Technology type: Gas Chromatography Mass Spectrometry

Snapshots: No snapshots

Model prediction of the conversion of 3PG to fructose-6-phosphate and the gluconeogenic pathway intermediates.
https://jjj.bio.vu.nl/models/experiments/kouril3_experiment-user/simulate

Modelling the degradation of BPG, GAP and DHAP at high temperature

No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Enzymatic Activity Measurements

Snapshots: No snapshots

PGK - GAPDH models

Contributor: Jacky Snoep

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Experimental data for the yeast PGK incubations at 30C, with and without recycling of ATP.

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

A model for the PGK reaction of yeast in presence or absence of the ATP recycling reactions

Contributor: Jacky Snoep

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Changes in metabolite concentrations were either quantified via 31P NMR or enzymatically

PGK 70C model

Contributor: Jacky Snoep

Biological problem addressed: Model Analysis Type

Snapshots: No snapshots

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

Contributor: Jacky Snoep

Biological problem addressed: Enzymology

Snapshots: No snapshots

No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

No description specified

Contributor: Theresa Kouril

Assay type: Experimental Assay Type

Technology type: Technology Type

Snapshots: No snapshots

In vitro reconstitution of the PGK, GAPHD, TPI and FBPAase enzymes from S. solfataricus

Temperature degradation of BPG, GAP and DHAP

Contributor: Jacky Snoep

Assay type: Metabolite Concentration

Technology type: Technology Type

Snapshots: No snapshots

Mathematical model for TPI kinetics, saturation with GAP and DHAP, and inhibition by 3PG and PEP

Contributor: Jacky Snoep

Biological problem addressed: Enzymology

Snapshots: No snapshots

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