Data files

TRK1, TRK2, ENA1-5

TRK1, TRK2, NHA1, ENA1-5

TRK1, TRK2, TOK1, NHA1, ENA1-5

For each modeled 3D structure of LHD (see Part 1: 3D structure modeling for LDH enzymes) was computed electrostatic potential by using the UHBD program. The files are in GRD format (binary) and can be visualized with the graphical programs as CHIMERA or VMD.

Monitoring of end products ethanol, acetone, butanol, acetate and butyrate during master fermentation of Clostridium acetobutylicum at the steady state pH values 5.5, 5.3, 5.1, 4.9 and 4.7.

Monitoring of end products in mM of ethanol, acetone, butanol, acetate and butyrate during master fermentation of Clostridium acetobutylicum at the steady state pH values 5.7 (acidogenesis) and 4.5 (solventogenesis).

This .csv file contains the entire transcriptional dataset of the aerobic to anaerobic transition. The values shown are the gene-expression ratio at 2, 5, 10, 15 and 20 minutes relative to the 0 minute timepoint.

This .csv file contains the entire transcriptional dataset of the anaerobic to aerobic transition. The values shown are the gene-expression ratio at 2, 5, 10, 15 and 20 minutes relative to the 0 minute timepoint.

The ArcA phosphorylation state was determined for mutants with linear respiratory chain at defined aerobiosis levels.

by-product formation rates and glucose uptake rates of mutants with linear electron transport chain at different aerobiosis levels

Gene expression levels in mutants with linear ETC were determined by RealTime RT-PCR

transcriptome, glucose, biomass data inside

Output files of phosphate probe binding on the surface of LDH from lactococcus lactis type 1. File with extension XPLOR can be visualized with a program VMD to identify the most favorable position for the phosphate binding. This relates to the Model "Part 4".

Excel sheet template : concentrations of intracellular metabolites

Experimental data for 3PG conversion to fructose-6-phosphase in reconstituted systems of gluconeogenesis of S. solfataricus

extracellular metabolites concentrations [mM] measured by 1H-NMR i