Data files

Metabolic changes of 26 intracellular metabolites extracted from T. b. brucei 427 wild type and arginine kinase knockout cells exposed to methylene blue

Metabolic changes of 26 intracellular metabolites extracted from T. b. brucei 427 wild type and arginine kinase knockout cells under high pH stress (pH8.7)

Intracellular concentrations of 50 metabolites measured by LC-MS using isotope ratio based mass spectrometry technique

The Table represents the simulation results of how the presence of phosphate ions (Pi) in the solution might affect the activity of four LDH enzymes. This includes the algorithmic analysis of the binding energies values computed by the GRID program (see Part 4, model) for each enzyme in presence and absence of FBP molecule at pH 6 and 7. The analysis was performed by using the algorithm proposed in Part 5, model.

TbTryS activity was measured at 37°C in the in vivo-like buffer. All substrate stock solutions were prepared in the in vivo-like buffer and the pH was adjusted to 7.0. The assays were performed in a final volume of 2 ml and contained 0.2 mM NADH, 1 mM phosphoenolpyruvate, 4 units pyruvate kinase, 4 units L-lactate dehydrogenase, 0.17 µM TbTryS, 2.1 mM ATP and varying amounts of GSH, and Spd.

The file contains the initial rate measurements of TbTryS obtained under different substrate and product initial concentrations.

Final version of the Agenda: All Hands PALs meeting on 21-22 of May 2012 in Warnemünde/Rostock.

raw data OD600

amino acid auxotrophies as determined for S. pyogenes, E. faecalis, L. lactis and L. plantarum (ATCC, NC8, JDM1 and WCFS1) subsequent inoculation (3x) in CDM

raw data OD600 of amino acid auxotrophy exps S. pyogenes

amino acid auxotrophy for streptococcus pyogenes in CDM-IMIKRO (not CDM-LAB) as function of final growth yield (OD600 at stationary phase) in 96well plates (200 ul culture)

S. pneumoniae Control1 DNA-Seq PE1

S. pneumoniae Control 1 DNA PE2

S. pneumoniae Control 2 DNA PE1

S. pneumoniae Control 2 DNA PE2

S. pneumoniae HPUra 1 DNA PE1

S. pneumoniae HPUra 1 DNA PE2

S. pneumoniae HPUra 2 DNA PE1

S. pneumoniae HPUra 2 DNA PE2

S. pneumoniae kanamycin DNA PE1

S. pneumoniae kanamycin DNA PE2

E. coli DNA-Seq Barcodes: control 1: GTGGCC control 2: GTTTCG Trimethoprim 1: CACTCA Trimethoprim 2: CAGGCG

E. coli RNA-Seq Barcodes: Control 1: AGTTCC Control 2: ATGTCA

E. coli RNA-Seq Barcodes: Trimethoprim 1: CCGTCC Trimethoprim 2: GTAGAG

S. pneumoniae RNA-Seq Barcodes: Control 1: CAGATC Control 2: ACTTGA

S. pneumoniae RNA-Seq Barcodes: HPUra 1: CAGATC HPUra 2: ACTTGA Kanamycin: AGTCAA

This document shows the ArcA phosphorylation levels at different aerobiosis units, measured using Phos-tag gels, western immunoblotting. Exposed films were quantitated using ImageJ and the results shown here.