Assays
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Submitter: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: K+ Starvation in Saccharomyces cerevisiae
Study: Transcriptional Profile
Extracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.
Submitter: Dong-Hyun Kim
Assay type: Extracellular Metabolite Concentration
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Metabolic flux measurement
Intracellular metabolites in T. brucei at different stage of cell growth have been quantified absolutely by isotope ratio based MS technique using uniformly 13C-labelled E. coli extract. This is the case study for method development of absolute quantification for metabolic flux analysis.
Submitter: Dong-Hyun Kim
Assay type: Metabolomics
Technology type: Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Metabolic flux measurement
Submitter: Michael Ederer
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Steady state studies for different oxygen avail...
Km values of pyruvate kinase of different organisms without/with allosteric effector molecules collected from literature.
Submitter: Stefan Henrich
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: The Attic
- Preparation of B. subtilis cultures
Inoculate cells from -80°C stocks in 10 ml time-lapse microscopy (TLM) medium (62 mM K2HPO4 , 44mM KH2PO4, 15 mM (NH4)2SO4, 6.5 mM sodium citrate, 0.8 mM MgSO4, 0.02 % casamino acids, 27.8 mM glucose, 0.1 mM L-tryptophan, the pH was set to 7 using a KOH solution) supplemented with antibiotics, if necessary. Grow the cells overnight in a shake flask (30°C, 225 rpm). The following morning, dilute the cells 1:10 in pre-warmed chemically defined medium (CDM) (62 ...
Submitter: Jan-Willem Veening
Assay type: Experimental Assay Type
Technology type: Imaging
Investigation: Wetlab approach to transcription fidelity