Assays
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Using PCA, three components, beam size 8. Clustering via MCL from Biolayout Express 3D
Data is taken from "Genome-Wide Gene Expression Analysis of the Switch between Acidogenesis and Solventogenesis in Continuous Cultures of Clostridium acetobutylicum." Grimmler et al. 2011 DOI: 10.1159/000320973
Submitter: Sebastian Curth
Biological problem addressed: Gene Expression
Investigation: The effect of pH upon the metabolic shift in Cl...
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Submitter: Finn Bacall
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: 1 hidden item
Study: 1 hidden item
Development of bioinformatic tools to investigate the role of transcription factors and 14-3-3 proteins in the regulation of genes involved in cation homeostasis.
Submitter: Falko Krause
Biological problem addressed: Model Analysis Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Bioinformatic studies
Submitter: Federico Rojas
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Perturbing the biological system: developmental...
Submitter: Falko Krause
Biological problem addressed: Model Analysis Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Promotor Anaysis
Submitter: Sebastian Curth
Biological problem addressed: Model Analysis Type
Investigation: Modular Model Building
Study: Automated Model Building
Elucidation of protein networks involved in the regulation of cation homeostasis using protein interaction datasets.
Submitter: Falko Krause
Biological problem addressed: Model Analysis Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Bioinformatic studies
To estimate the differnces of internal pH between the wild type, single trk1 and trk2 or double trk1 trk2 mutants, the pH-dependent variant of GFP pHluorin was expressed in cells from a multicopy plasmid, cells were grown under various conditions, and the cell fluorescence was monitored.
Submitter: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei exposed to methylene blue have been absolutely quantified using isotope ratio based MS technique.
Submitter: Dong-Hyun Kim
Assay type: Intracellular Metabolite Concentration
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
26 intracellular metabolites (amino acids, polyamines, TCA intermediates) in T. brucei under pH stress (pH8.7) have been absolutely quantified using isotope ratio based MS technique.
Submitter: Dong-Hyun Kim
Assay type: Intracellular Metabolite Concentration
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Metabolite profiling, quantification and flux q...
Study: Targeted metabolite analysis
intracellular metabolite measured by GC/MS and LC/MS
Submitter: Hanna Meyer
Assay type: Metabolomics
Technology type: NMR
Investigation: Multiomics study of Bacillus subtilis under osm...
Pilot experiment concerning metabolome of S. solfataricus was conducted in order to acquire SOPs regarding the technique and gain insight on differences in metabolite concentrations at 70 and 80C
Submitter: Pawel Sierocinski
Assay type: Metabolomics
Technology type: Gas Chromatography Mass Spectrometry
Investigation: Analysis of Central Carbon Metabolism of Sulfol...
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity. SigmaB itself is placed downstream of Pspac, inducible by IPTG. The lacZ reporter gene is downstream of Pctc promoter. IPTG concentrations of 0.1, 0.2 and 1 mM are added in mid-exponential phase at an OD of appr. 0.3. The whole experiment runs for about eight hours.
Submitter: Ulf Liebal
Assay type: Proteomics
Technology type: Technology Type
Investigation: The transition from growing to non-growing Baci...
We use BSA115 strain which lacks RsbU and RsbW proteins. Therefore, there is limited post-transcriptional regulation of sigmaB activity.
There occurs an unexpected drop in the beta-Gal activity after sigB induction. This modelling effort aims to clarify the reasons.
Submitter: Ulf Liebal
Biological problem addressed: Gene Expression
Investigation: The transition from growing to non-growing Baci...