Assays
What is an Assay?Filters
Kinetic characterisation of fructose 1,6-bisphosphate aldolase phosphatase
Submitter: Jacky Snoep
Assay type: Enzymatic Assay
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model Gluconeogenesis
Mathematical model for FBPAase kinetics, saturation with DHAP and GAP
Submitter: Jacky Snoep
Biological problem addressed: Enzymology
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model Gluconeogenesis
In order to get uniform data from all geographically separated partners in a consortium, central fermentation facility has been set-up. Based on empirical data, standard procedures for growing S. solfataricus cells have been developed.
Submitter: Pawel Sierocinski
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Analysis of Central Carbon Metabolism of Sulfol...
Submitter: Praveen kumar Sappa
Assay type: Glucose Pulse
Technology type: Technology Type
Investigation: The transition from growing to non-growing Baci...
This experiment uses a low-copy plasmid based system (MG1655 Δlac FF(-41.5)/RW50) for measuring FNR activity. Initial acetate calibration of the chemostat with the MG1655 Δlac strain was carried out, with β-galactosidase activity from the FF(-41.5)/RW50 reporter plasmid measured at 100%, 80%, 50%, 20% and 0% aerobiosis levels. Finally, the aerobiosis levels were re-determined by calculating the actual acetate flux in the sampled chemostat runs.
Note: the strain used (MG1655 Δlac) is not the same ...
Submitter: Michael Ederer
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Steady state studies for different oxygen avail...
