Assays
What is an Assay?Filters
Cellular size and granularity (measured by FACS) during glucose pulse. Glucose pulse was performed in anaerobically growing yeast Saccharomyces cerevisiae in steady state chemostat (D = 0.1 h-1) and transent concentrations of the extra- and intracellular metabolites from central carbon metabolism (e.g. glycolysis, PPP, glycerol, purines, etc) were measured.
Submitter: Maksim Zakhartsev
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Kinetic analysis of metabolic system using tran...
E. coli MG1655 and ∆sdhC and ∆frdA isogenic mutant strains were characterized in batch growth curves aerobic and anaerobically. Optical density, glucose consumption and by-product accumulation were measured during growth.
Submitter: Sonja Steinsiek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Steady state studies for different oxygen avail...
Enzymes involved in butanediol formation from pyruvate in L. Lactis and E. faecalis were characterized with respect to their Km's for their substrates and their Vmaxes
Submitter: Martijn Bekker
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: The Attic
S. pyogenes wildetype, an arcA- and a glnA-deletion mutants were grown in CDM-LAB cultures at pH 6.5 and 7.5 and at a growth rate of 0.05
Submitter: Antje Sieg
Assay type: Experimental Assay Type
Technology type: HPLC
Investigation: Amino acid metabolism of four LAB species: Stre...
Mutant strains which lack one or more of the glucose transport systems were analyzed in aerobic chemostat cultures and compared to batch cultures.
Submitter: Sonja Steinsiek
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Analysis of the glucose transport in Escherichi...
This Excel template is an example taken from the GEO web site (http://www.ncbi.nlm.nih.gov/geo/info/spreadsheet.html#GAtemplates) which has been modified to conform to the SysMO JERM (Just Enough Results Model). Using templates helps with searching and comparing data as well as making it easier to submit data to public repositories for publications.
Submitter: Katy Wolstencroft
Assay type: Transcriptomics
Technology type: ChIP-on-chip
Investigation: Creating data sheet template for 'omics data
We will compare two different procedures to extract ATP from yeast cells: Standard kit procedure (hot Tris/EDTA) and Serrano procedure (cold perchloric acid). In addition we have tested different condition as it turned out that some are important.
Submitter: Walter Glaser
Assay type: Metabolomics
Technology type: Imaging
Investigation: Effect of Benzoic Acid on ATP Levels
Samples obtained form the central fermentation facility of Sulfosys have been compared using iTRAQ (isobaric tag for relative and absolute quantification). A pilot experiment resulted in creation of SOP and initial data on cells grown at 70 and 80C
Submitter: Pawel Sierocinski
Assay type: Protein Expression Profiling
Technology type: Isotope Ratio Mass Spectrometry
Investigation: Analysis of Central Carbon Metabolism of Sulfol...
Investigation of all steady state pH-values between pH 5.7 and 4.5 (pH 5.5, 5.3, 5.1, 4.9, 4.7).
Submitter: Sara Jabbari
Assay type: Proteomics
Technology type: Technology Type
Investigation: The effect of pH upon the metabolic shift in Cl...
Study: Effect of pH on the proteome
Comparison of the transcriptome at steady state in acidogenesis and at steady state in solventogenesis.
Submitter: Sara Jabbari
Assay type: Genomics
Technology type: Microarray
Investigation: The effect of pH upon the metabolic shift in Cl...
Investigation of the dynamic switch at pH values between 5.8 and 4.5 (pH 5.5, 5.3, 5.1, 4.9, 4.7 and 4.5).
Submitter: Sara Jabbari
Assay type: Genomics
Technology type: Microarray
Investigation: The effect of pH upon the metabolic shift in Cl...
The pilot experiment was conducted in order to create SOPs and to gain insight in transcriptome of cells grown at 70 and 80C
Submitter: Pawel Sierocinski
Assay type: Transcriptional Profiling
Technology type: Microarray
Investigation: Analysis of Central Carbon Metabolism of Sulfol...
In order to construct an in vivo-like buffer for S. pyogenes, the intracellular concentrations of Fe, K, Mg, Mn Na, P and S elements were determined via ICP-AES (inductively coupled plasma atomic emissionspectroscopy) method at the Institute of Land Use, University of Rostock. The samples for the analysis were obtained from a steady state culture grown on CDM-LAB with glucose.
Submitter: Araz Zeyniyev
Assay type: Metabolite Profiling
Technology type: Inductively Coupled Plasma Mass Spectrometry
Investigation: Amino acid metabolism of four LAB species: Stre...
We here create a kinetic model for a single enzyme within the T. brucei trypanothione synthesis pathway, the enzyme trypanothione synthetase based on the insights from the laboratory experiments
Submitter: Jurgen Haanstra
Biological problem addressed: Enzymology
Investigation: Kinetic understanding of the T. brucei trypanot...
The current-voltage relation for Trk1,2 will be determined according to the conditions of Kuroda et al.
Submitter: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Current-voltage relation
The current-voltage relation for Trk1,2 will be determined for various external potassium concentrations.
Submitter: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: NHA1 Transport System in Saccharomyces cerevisiae
Study: Current-voltage relations
Submitter: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: TRK1,2 Transport Systems of Saccharomyces cerev...
Study: Current-voltage relation
Current- voltage relations for will be determined for various internal potassium concentrations.
Submitter: Falko Krause
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: NHA1 Transport System in Saccharomyces cerevisiae
Study: Current-voltage relations